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1.
Transgenic Res ; 19(4): 691-701, 2010 Aug.
Article in English | MEDLINE | ID: mdl-19937380

ABSTRACT

Supv3L1 is an evolutionarily conserved helicase that plays a critical role in the mitochondrial RNA surveillance and degradation machinery. Conditional ablation of Supv3L1 in adult mice leads to premature aging phenotypes including loss of muscle mass and adipose tissue and severe skin abnormalities. To get insights into the spatial and temporal expression of Supv3L1 in the mouse, we generated knock-in and transgenic strains in which an EGFP reporter was placed under control of the Supv3L1 native promoter. During development, expression of Supv3L1 begins at the blastocyst stage, becomes widespread and strong in all fetal tissues and cell types, and continues during postnatal growth. In mature animals reporter expression is only slightly diminished in most tissues and continues to be highly expressed in the brain, peripheral sensory organs, and testis. Together, these data confirm that Supv3L1 is an important developmentally regulated gene, which continues to be expressed in all mature tissues, particularly the rapidly proliferating cells of testes, but also in the brain and sensory organs. The transgenic mice and cell lines derived from them constitute a valuable tool for the examination of the spatial and temporal aspects of Supv3L1 promoter activity, and should facilitate future screens for small molecules that regulate Supv3L1 expression.


Subject(s)
DEAD-box RNA Helicases/genetics , DEAD-box RNA Helicases/metabolism , Animals , Cells, Cultured , Female , Gene Expression , Gene Expression Regulation, Developmental , Gene Transfer Techniques , Green Fluorescent Proteins/genetics , Green Fluorescent Proteins/metabolism , Male , Mice , Mice, Transgenic , Mitochondrial Proteins/genetics , Mitochondrial Proteins/metabolism , Models, Biological , Pregnancy , Tissue Distribution
2.
Hum Mol Genet ; 18(23): 4629-39, 2009 Dec 01.
Article in English | MEDLINE | ID: mdl-19744960

ABSTRACT

Grb10-Interacting GYF Protein 2 (GIGYF2) was initially identified through its interaction with Grb10, an adapter protein that binds activated IGF-I and insulin receptors. The GIGYF2 gene maps to human chromosome 2q37 within a region linked to familial Parkinson's disease (PARK11 locus), and association of GIGYF2 mutations with Parkinson's disease has been described in some but not other recent publications. This study investigated the consequences of Gigyf2 gene disruption in mice. Gigyf2 null mice undergo apparently normal embryonic development, but fail to feed and die within the first 2 post-natal days. Heterozygous Gigyf2(+/-) mice survive to adulthood with no evident metabolic or growth defects. At 12-15 months of age, the Gigyf2(+/-) mice begin to exhibit motor dysfunction manifested as decreased balance time on a rotating horizontal rod. This is associated with histopathological evidence of neurodegeneration and rare intracytoplasmic Lewy body-like inclusions in spinal anterior horn motor neurons. There are alpha-synuclein positive neuritic plaques in the brainstem and cerebellum, but no abnormalities in the substantia nigra. Primary cultured embryo fibroblasts from Gigyf2 null mice exhibit decreased IGF-I-stimulated IGF-I receptor tyrosine phosphorylation and augmented ERK1/2 phosphorylation. These data provide further evidence for an important role of GIGYF2 in age-related neurodegeneration and IGF pathway signaling.


Subject(s)
Carrier Proteins/genetics , Gene Silencing , Insulin-Like Growth Factor I/metabolism , Nerve Degeneration/metabolism , Signal Transduction , Animals , Carrier Proteins/metabolism , Cells, Cultured , Disease Models, Animal , Female , Fibroblasts/metabolism , Humans , Insulin-Like Growth Factor I/genetics , Male , Mice , Mice, Inbred C57BL , Mice, Knockout , Mitogen-Activated Protein Kinase 3/genetics , Mitogen-Activated Protein Kinase 3/metabolism , Motor Activity , Nerve Degeneration/embryology , Nerve Degeneration/genetics , Nerve Degeneration/physiopathology , Receptor, IGF Type 1/genetics , Receptor, IGF Type 1/metabolism
3.
Biochim Biophys Acta ; 1789(5): 413-21, 2009 May.
Article in English | MEDLINE | ID: mdl-19362612

ABSTRACT

Chromatin modifications are essential for directing transcription during embryonic development. Bromodomain-containing protein 2 (Brd2; also called RING3 and Fsrg1) is one of four BET (bromodomain and extra-terminal domain) family members known to selectively bind acetylated histones H3 and H4. Brd2 associates with multiple subunits of the transcriptional apparatus including the mediator, TFIID and Swi/Snf multiprotein complexes. While molecular interactions of Brd2 are known, the functions of Brd2 in mammalian embryogenesis remain unknown. In developing a mouse model deficient in Brd2, we find that Brd2 is required for the completion of embryogenesis and proper neural tube closure during development. Embryos lacking Brd2 expression survive up to embryonic day 13.5, soon after mid-gestation, and display fully penetrant neurulation defects that largely result in exencephaly of the developing hindbrain. In this study, we find that highest expression of Brd2 is detected in the developing neural tube, correlating with the neural tube defects found in Brd2-null embryos. Additionally, embryos lacking Brd2 expression display altered gene expression programs, including the mis-expression of multiple genes known to guide neuronal development. Together these results implicate essential roles for Brd2 as a critical integrator of chromatin structure and transcription during mammalian embryogenesis and neurogenesis.


Subject(s)
Chromatin/genetics , Embryonic Development/genetics , Neural Tube Defects/genetics , Protein Serine-Threonine Kinases/genetics , Animals , Apoptosis/genetics , Cell Growth Processes/genetics , Cell Line , Chromatin/metabolism , Chromosomal Proteins, Non-Histone , Embryo, Mammalian , Embryonic Stem Cells/physiology , Female , Gene Expression Profiling , Gene Expression Regulation, Developmental , Male , Mice , Mice, Inbred C57BL , Mutation , Neural Crest/embryology , Neural Crest/pathology , Neural Tube/embryology , Neural Tube/pathology , Neural Tube Defects/embryology , Neural Tube Defects/pathology , Polymerase Chain Reaction , Pregnancy , Protein Serine-Threonine Kinases/biosynthesis , Protein Serine-Threonine Kinases/deficiency , Protein Serine-Threonine Kinases/metabolism , Transcription Factors
4.
Mamm Genome ; 20(2): 92-108, 2009 Feb.
Article in English | MEDLINE | ID: mdl-19145458

ABSTRACT

Supv3L1 is a conserved and ubiquitously expressed helicase found in numerous tissues and cell types of many species. In human cells, SUPV3L1 was shown to suppress apoptotic death and sister chromatid exchange, and impair mitochondrial RNA metabolism and protein synthesis. In vitro experiments revealed binding of SUPV3L1 to BLM and WRN proteins, suggesting a role in genome maintenance processes. Disruption of the Supv3L1 gene in the mouse has been reported to be embryonic lethal at early developmental stages. We generated a conditional mouse in which the phenotypes associated with the removal of exon 14 can be tested in a variety of tissues. Disruption mediated by a Mx1 promoter-driven Cre displayed a postnatal growth delay, reduced lifespan, loss of adipose tissue and muscle mass, and severe skin abnormalities manifesting as ichthyosis, thickening of the epidermis, and atrophy of the dermis and subcutaneous tissue. Using a tamoxifen-activatable Esr1/Cre driver, Supv3L1 disruption resulted in growth retardation and aging phenotypes, including loss of adipose tissue and muscle mass, kyphosis, cachexia, and premature death. Many of the abnormalities seen in the Mx1-Cre mice, such as hyperkeratosis characterized by profound scaling of feet and tail, could also be detected in tamoxifen-inducible Cre mice. Conditional ablation of Supv3L1 in keratinocytes confirmed atrophic changes in the skin and ichthyosis-like changes. Together, these data indicate that Supv3L1 is important for the maintenance of the skin barrier. In addition, loss of Supv3L1 function leads to accelerated aging-like phenotypes.


Subject(s)
Adipose Tissue/abnormalities , DEAD-box RNA Helicases/physiology , Skin Abnormalities/pathology , Adipose Tissue/embryology , Adipose Tissue/growth & development , Aging, Premature/genetics , Animals , Cachexia/genetics , DEAD-box RNA Helicases/genetics , Epidermis/embryology , Epidermis/growth & development , Epidermis/pathology , Humans , Ichthyosis/genetics , Kyphosis/genetics , Longevity/genetics , Mice , Mice, Knockout , Muscle, Skeletal/abnormalities , Skin/embryology , Skin/growth & development , Skin/pathology , Skin Abnormalities/genetics , Tamoxifen/pharmacology
5.
Eur J Neurosci ; 30(11): 2064-76, 2009 Dec 03.
Article in English | MEDLINE | ID: mdl-20128845

ABSTRACT

We report here the construction of a novel knock-in mouse expressing chimeric alpha3 nicotinic acetylcholine receptor (nAChR) subunits with pharmacological sensitivity to alpha-bungarotoxin (alphaBTX). Sensitivity was generated by substituting five amino acids in the loop C (beta9-beta10) region of the mouse alpha3 subunit with the corresponding residues from the alpha1 subunit of the muscle type receptor from Torpedo californica. To demonstrate the utility of the underlying concept, expressed alpha3[5] subunits were characterized in the superior cervical ganglia (SCG) of homozygous knock-in mice, where the synaptic architecture of postsynaptic alpha3-containing nAChR clusters could now, for the first time, be directly visualized and interrogated by live-staining with rhodamine-conjugated alphaBTX. Consistent with the postsynaptic localization of ganglionic nAChRs, the alphaBTX-labeled puncta colocalized with a marker for synaptic varicosities. Following in vivo deafferentation, these puncta persisted but with significant changes in intensity and distribution that varied with the length of the recovery period. Compound action potentials and excitatory postsynaptic potentials recorded from SCG of mice homozygous for alpha3[5] were abolished by 100 nmalphaBTX, even in an alpha7 null background, demonstrating that synaptic throughput in the SCG is completely dependent on the alpha3-subunit. In addition, we observed that the genetic background of various inbred and outbred mouse lines greatly affects the functional expression of alpha3[5]-nAChRs, suggesting a powerful new approach for exploring the molecular mechanisms underlying receptor assembly and trafficking. As alphaBTX-sensitive sequences can be readily introduced into other nicotinic receptor subunits normally insensitive to alphaBTX, the findings described here should be applicable to many other receptors.


Subject(s)
Bungarotoxins/pharmacology , Mice, Knockout , Neurons/drug effects , Receptors, Nicotinic/deficiency , Superior Cervical Ganglion/cytology , Acetylcholine/pharmacology , Age Factors , Animals , Animals, Newborn , Autonomic Denervation/methods , Binding Sites/drug effects , Binding Sites/genetics , Bungarotoxins/metabolism , Cells, Cultured , Cholinergic Agents/pharmacology , Gene Expression Regulation, Developmental/drug effects , Gene Expression Regulation, Developmental/genetics , Intracellular Signaling Peptides and Proteins/metabolism , Membrane Potentials/drug effects , Membrane Potentials/genetics , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Neurofilament Proteins/metabolism , Neurons/physiology , Patch-Clamp Techniques/methods , Phenotype , Synaptic Transmission/drug effects , Synaptic Transmission/genetics , Time Factors , Vesicular Acetylcholine Transport Proteins/metabolism
6.
BMC Physiol ; 8: 4, 2008 Feb 29.
Article in English | MEDLINE | ID: mdl-18312674

ABSTRACT

BACKGROUND: Myocardial hypoxic-ischemic injury is the cause of significant morbidity and mortality worldwide. The cardiomyocyte response to hypoxic-ischemic injury is known to include changes in cell cycle regulators. The cyclin-dependent kinase inhibitor p57Kip2 is involved in cell cycle control, differentiation, stress signaling and apoptosis. In contrast to other cyclin-dependent kinase inhibitors, p57Kip2 expression diminishes during postnatal life and is reactivated in the adult heart under conditions of cardiac stress. Overexpression of p57Kip2 has been previously shown to prevent apoptotic cell death in vitro by inhibiting stress-activated kinases. Therefore, we hypothesized that p57Kip2 has a protective role in cardiomyocytes under hypoxic conditions. To investigate this hypothesis, we created a transgenic mouse (R26loxpTA-p57k/+) that expresses p57Kip2 specifically in cardiac tissue under the ventricular cardiomyocyte promoter Mlc2v. RESULTS: Transgenic mice with cardiac specific overexpression of p57Kip2 are viable, fertile and normally active and their hearts are morphologically indistinguishable from the control hearts and have similar heart weight/body weight ratio. The baseline functional parameters, including left ventricular systolic pressure (LVSP), left ventricular end diastolic pressure (LVEDP), LVdp/dtmax, heart rate (HR) and rate pressure product (RPR) were not significantly different between the different groups as assessed by the Langendorff perfused heart preparation. However, after subjecting the heart ex vivo to 30 minutes of ischemia-reperfusion injury, the p57Kip2 overexpressing hearts demonstrated preserved cardiac function compared to control mice with higher left ventricular developed pressure (63 +/- 15 vs 30 +/- 6 mmHg, p = 0.05), rate pressure product (22.8 +/- 4.86 vs 10.4 +/- 2.1 x 103bpm x mmHg, p < 0.05) and coronary flow (3.5 +/- 0.5 vs 2.38 +/- 0.24 ml/min, p <0.05). CONCLUSION: These data suggest that forced cardiac expression of p57Kip2 does not affect myocardial growth, differentiation and baseline function but attenuates injury from ischemia-reperfusion in the adult mouse heart.


Subject(s)
Cyclin-Dependent Kinase Inhibitor p57/metabolism , Myocardial Reperfusion Injury/metabolism , Myocardial Reperfusion Injury/pathology , Myocytes, Cardiac/metabolism , Myocytes, Cardiac/pathology , Animals , Cardiotonic Agents/metabolism , Cells, Cultured , Cyclin-Dependent Kinase Inhibitor p57/genetics , Mice , Mice, Transgenic
7.
Cell Signal ; 20(1): 1-9, 2008 Jan.
Article in English | MEDLINE | ID: mdl-17706925

ABSTRACT

The Raf kinase inhibitory protein 1 (RKIP-1) and its orthologs are conserved throughout evolution and widely expressed in eukaryotic organisms. In its non-phosphorylated form RKIP-1 negatively regulates the Raf/MEK/ERK pathway by interfering with the activity of Raf-1. In its phosphorylated state, RKIP-1 dissociates from Raf-1 and inhibits GRK-2, a negative regulator of G-protein coupled receptors (GPCRs). Available data indicate that the phosphorylation of RKIP-1 by PKC can stimulate both the Raf/MEK/ERK and GPCR pathways. RKIP-1 has also been implicated as a negative regulator of the NF-kappaB pathway. Recent studies have shown that phosphorylated RKIP-1 binds to the centrosomal and kinetochore regions of metaphase chromosomes, where it may be involved in regulating the partitioning of chromosomes and the progression through mitosis. The collective evidence indicates that RKIP-1 regulates the activity and mediates the crosstalk between several important cellular signaling pathways. A variety of ablative interventions suggest that reduced RKIP-1 function may influence metastasis, angiogenesis, resistance to apoptosis, and genome integrity. Attenuation of RKIP-1 may also affect cardiac and neurological functions, spermatogenesis, sperm decapacitation, and reproductive behavior. In this review, the role of RKIP-1 in cellular signaling, and especially its functions revealed using a mouse knockout model, are discussed.


Subject(s)
Phosphatidylethanolamine Binding Protein/physiology , Signal Transduction/physiology , Alzheimer Disease/physiopathology , Animals , Central Nervous System/physiology , Mice , Mice, Knockout , Neoplasms/physiopathology , Phosphatidylethanolamine Binding Protein/genetics , Reproduction/physiology , Spermatogenesis/physiology
8.
Med Sci Monit ; 13(11): LE18-18, 2007 Nov.
Article in English | MEDLINE | ID: mdl-17968306
9.
Mech Ageing Dev ; 128(11-12): 609-17, 2007.
Article in English | MEDLINE | ID: mdl-17961633

ABSTRACT

The SUV3 gene is present in all eukaryotes and encodes an RNA/DNA helicase which operates both in mitochondria and cell nuclei. To assess its function in mammals we generated a mouse mutant strain in which the 3' part of the SUV3 gene is disrupted. The mutated allele is a hypomorph transmitted from one generation to another at a frequency about 35% lower than expected while mice homozygous for the mutation die in utero before midgestation. Using ELISA binding assays we show that human SUV3 protein interacts with human WRN and BLM helicases. The binding to BLM protein was 10-fold stronger (with a K(d) of 0.5nM) than to WRN protein (K(d) of 5nM). Silencing of the SUV3 gene in the human cell line HeLa resulted in elevation of homologous recombination as measured by the frequency of sister chromatid exchange during mitotic cell division. These results indicate that the SUV3 protein is required in mammalian development and in somatic cells participates in genome maintenance through interaction with other genome fidelity housekeepers.


Subject(s)
Adenosine Triphosphatases/metabolism , DEAD-box RNA Helicases/metabolism , DNA Helicases/metabolism , Embryo Loss/genetics , Gene Silencing , Mitosis/genetics , Sister Chromatid Exchange , Amino Acid Sequence , Animals , DEAD-box RNA Helicases/genetics , Embryo Loss/metabolism , Embryonic Stem Cells/metabolism , Exodeoxyribonucleases , Gestational Age , HeLa Cells , Humans , Mice , Mice, Inbred C57BL , Mice, Knockout , Molecular Sequence Data , Protein Binding , RNA Interference , RNA, Small Interfering/metabolism , RecQ Helicases/metabolism , Transfection , Werner Syndrome Helicase
11.
J Androl ; 28(6): 883-90, 2007.
Article in English | MEDLINE | ID: mdl-17554109

ABSTRACT

Raf kinase inhibitor protein-1 (RKIP-1) belongs to the phosphatidyl ethanolamine-binding family of proteins (PEBP), which are highly conserved throughout evolution and widely expressed in tissues of mammalian organisms. RKIP-1 is a modulator of extracellular signal-regulated kinase (ERK), nuclear factor-kappa B (NF-kappaB), and G protein coupled receptor (GPCR) signaling cascades and is implicated as a factor in numerous physiological processes and disease states including metastasis. Testicular germ cells also express high levels of RKIP mRNA during spermatogenesis, particularly from late pachytene spermatocytes through step 15 elongate spermatids. Therefore, the sensitivity of spermatogenesis to injury was compared in wild-type and RKIP-1(-/-) mice. Unlike what has been described with tumor suppressors such as p53, RKIP-1(-/-) and wild-type mice were equally sensitive to germ cell toxicity by x-irradiation as assessed by terminal deoxynucleotidyl transferase biotin-deoxyuridine triphosphate (dUTP) nick end labeling (TUNEL) positivity 9 hours after a 5 Gy exposure and testicular spermatid head counts 15.5 days after 0.5 Gy exposure. Recent findings also indicate that RKIP is a decapacitation factor receptor on sperm. The present study demonstrates that sperm from RKIP-deficient mice are precociously capacitated compared with their wild-type counterparts. Data from mating experiments indicate decreased reproduction rates between crosses of RKIP-1(-/-) male mice and either heterozygous or RKIP-1(-/-) females. Furthermore, RKIP immunolocalization of epididymal sperm supports transfer of the protein from germ cell cytoplasm to the sperm via the cytoplasmic droplet during epididymal transport. Overall, these studies indicate an important role for RKIP in reproduction as a modulator of capacitation but not in the regulation of testicular injury.


Subject(s)
Infertility, Male/genetics , Phosphatidylethanolamine Binding Protein/deficiency , Sperm Capacitation/physiology , Testis/injuries , Animals , Epididymis/pathology , Male , Mice , Mice, Knockout , Phosphatidylethanolamine Binding Protein/genetics , Sperm Count , Sperm Head , Spermatozoa/cytology , Spermatozoa/pathology
12.
Brain Res Bull ; 71(6): 559-67, 2007 Mar 30.
Article in English | MEDLINE | ID: mdl-17292798

ABSTRACT

Raf kinase inhibitory protein (RKIP-1) is involved in the regulation of the MAP kinase, NF-kappaB, and GPCR signaling pathways. It is expressed in numerous tissues and cell types and orthologues have been documented throughout the animal and plant kingdoms. RKIP-1 has also been reported as an inhibitor of serine proteases, and a precursor of a neurostimulatory peptide. RKIP-1 has been implicated as a suppressor of metastases in several human cancers. We generated a knockout strain of mice to further assess RKIP-1's function in mammals. RKIP-1 is expressed in many tissues with the highest protein levels detectable in testes and brain. In the brain, expression was ubiquitous in limbic formations, and homozygous mice developed olfaction deficits in the first year of life. We postulate that RKIP-1 may be a modulator of behavioral responses.


Subject(s)
Brain/metabolism , Gene Expression Regulation, Developmental/genetics , Olfaction Disorders/genetics , Phosphatidylethanolamine Binding Protein/genetics , Testis/metabolism , Animals , Brain/anatomy & histology , Brain/growth & development , Cell Line , Embryonic Stem Cells/cytology , Embryonic Stem Cells/metabolism , Female , Genes, Recessive/genetics , Genes, Reporter/genetics , Inheritance Patterns/genetics , Limbic System/anatomy & histology , Limbic System/growth & development , Limbic System/metabolism , Male , Mice , Mice, Inbred C57BL , Mice, Knockout , Mutation/genetics , Organ Specificity , Testis/cytology , Testis/growth & development
13.
Biochem Genet ; 43(5-6): 321-33, 2005 Jun.
Article in English | MEDLINE | ID: mdl-16144308

ABSTRACT

Meiotic recombination between homologous chromosomes can be suppressed within a chosen segment by a regional inversion. In mice, this feature can be engineered and conveniently used in genetic screens to maintain chemically induced mutations within the homologous chromosome. The efficiency of an inversion-based mutagenesis screen can be substantially enhanced provided that the inversion chromosome and its wild-type (WT) homologue are both visibly tagged by two different coat color markers. Dual tagging eliminates labor associated with molecular genotyping. Previously, we reported the generation of the In(11)10Brd strain of mice carrying K14-agouti tagging a 30-cM inversion between the Trp53 and Egfr loci on mouse chromosome 11. Since K14-agouti causes yellowing of ears and tails, the In(11)10Brd mice are easily distinguishable from their WT littermates. In this paper, we describe the construction of a second strain of mice that carry the enhanced green fluorescent protein (EGFP) transgene at the Egfr locus. The EGFP carriers are visually recognizable by emitting green fluorescent light upon UV illumination. We found that the EGFP function was transmitted from one generation to another with expected Mendelian frequencies, and no detrimental effects of EGFP expression were detected in hemizygous or homozygous animals. The EGFP mice together with the previously generated In(11)10Brd inversion carriers constitute a complete set of reagents required for initiation of a regional ENU mutagenesis screen to address functionally more than one-third of mouse chromosome 11.


Subject(s)
Green Fluorescent Proteins/genetics , Recombination, Genetic/genetics , Animals , Cell Line , Chromosome Inversion/genetics , Female , Genetic Engineering/methods , Genetic Vectors , Male , Mice , Mice, Transgenic
14.
Pol Merkur Lekarski ; 16(93): 205-7, 2004 Mar.
Article in Polish | MEDLINE | ID: mdl-15190590

ABSTRACT

The sequencing of the human genome was completed in 2003. These data will affect all aspects of biological and medical sciences. Since almost all the genes of the genome are known, the diagnosis and therapy of the future is expected to evolve towards more personalized and efficient methods. Before these medical advancements become applicable, functional genomics must successfully determine the cellular role all the genes in the genome. Comprehensive functional studies will require suitable animal models. Although many mammalian species are used in biological and medical experiments, the mouse emerges as a key model organism in studies of human genes. The power of the mouse model system comes from its extensive physiological and pathological similarities to humans and from technological advancements offered by mouse genetics. Recently, a project called ENCODE was launched to accelerate this monumental effort of gene analysis. Once gene analysis is completed, the face of medicine will change markedly. The greatest impact is expected on the front of individual diagnosis of disease genes and personalized treatments of patients.


Subject(s)
Genomics/trends , Human Genome Project , Animals , Genome, Human , Humans , Mice/genetics
15.
Genomics ; 83(2): 303-10, 2004 Feb.
Article in English | MEDLINE | ID: mdl-14706459

ABSTRACT

Segmental inversions causing recombination suppression are an essential feature of balancer chromosomes. Meiotic crossing over between homologous chromosomes within an inversion interval will lead to nonviable gametes, while gametes generated from recombination events elsewhere on the chromosome will be unaffected. This apparent recombination suppression has been widely exploited in genetic studies in Drosophila to maintain and analyze stocks carrying recessive lethal mutations. Balancers are particularly useful in mutagenesis screens since they help to establish the approximate genomic location of alleles of genes causing phenotypes. Using the Cre-loxP recombination system, we have constructed two mouse balancer chromosomes carrying 8- and 30-cM inversions between Wnt3 and D11Mit69 and between Trp53 and EgfR loci, respectively. The Wnt3-D11Mit69 inversion mutates the Wnt3 locus and is therefore homozygous lethal. The Trp53-EgfR inversion is homozygous viable, since the EgfR locus is intact and mutations in p53 are homozygous viable. A dominantly acting K14-agouti minigene tags both rearrangements, which enables these balancer chromosomes to be visibly tracked in mouse stocks. With the addition of these balancers to the previously reported Trp53-Wnt3 balancer, most of mouse chromosome 11 is now available in balancer stocks.


Subject(s)
Chromosome Inversion , Chromosomes , Recombination, Genetic , Animals , ErbB Receptors/genetics , Genetic Engineering/methods , Mice , Mice, Transgenic , Molecular Probes , Proteins/genetics , Tumor Suppressor Protein p53/genetics , Wnt Proteins , Wnt3 Protein
16.
Acta Biochim Pol ; 49(3): 553-69, 2002.
Article in English | MEDLINE | ID: mdl-12422227

ABSTRACT

Functional modeling of human genes and diseases requires suitable mammalian model organisms. For its genetic malleability, the mouse is likely to continue to play a major role in defining basic genetic traits and complex pathological disorders. Recently, gene targeting techniques have been extended towards developing new engineering strategies for generating extensive lesions and rearrangements in mouse chromosomes. While these advances create new opportunities to address similar aberrations observed in human diseases, they also open new ways of scaling-up mutagenesis projects that try to catalogue and annotate cellular functions of mammalian genes.


Subject(s)
Chromosomes/genetics , Genetic Techniques , Genome, Human , Genomics , Mice/genetics , Animals , Disease Models, Animal , Embryo, Mammalian/cytology , Genetic Phenomena/genetics , Humans , Mutagenesis/genetics , Species Specificity , Stem Cells/physiology , Transgenes/genetics
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