An improved procedure for purifying 5'-nucleotidase from various sources. Evidence for tissue and species differences in their molecular mass and affinity for F-actin.

5'-Nucleotidase from chicken gizzard smooth muscle has been extracted, using a sulfobetaine derivate of cholic acid, and purified to homogeneity by employing three chromatographic steps. It is shown that the purification scheme can be applied to 5'-nucleotidase from other sources, such as rat liver. On sodium dodecyl sulfate polyacrylamide gels, stained with silver nitrate, the purified enzyme from chicken gizzard shows a single polypeptide band with an apparent molecular mass of 79 kDa. The enzyme purified from rat liver exhibits a molecular mass of 73 kDa in agreement with published data [Bailyes, E.M., Soos, M., Jackson, P., Newby, A. C., Siddle, K. & Luzio, J.P. (1984) Biochem. J. 221, 369-377). Gel filtration, using non-denaturating detergent solutions, indicates that the native enzyme may exist as a homodimer (152 kDa) or homotetramer (310 kDa). Antibodies raised against the enzyme purified from chicken gizzard bind only 5'-nucleotidase, solubilized from chicken muscular sources, when immobilized, but not from chicken or rat liver. The existence of tissue specific variants of 5'-nucleotidase is therefore postulated and it appears that these particular isoforms can also be classified in membranous and secretory forms of 5'-nucleotidase. They also differ in their mode of interaction with actin. The AMPase activity of the membranous (= muscular) isoform is inhibited to a considerably higher percentage by F-actin than the enzyme isolated from rat liver.

Delaware river: evidence for its former extension to wilmington submarine canyon.

Seismic-reflection profiles indicate that during the Pleistocene the Delaware River flowed across the continental shelf east of Delaware Bay and emptied into Wilmington Submarine Canyon. The ancestral valley (width, 3 to 8 kilometers; relief, 10 to 30 meters) is buried, is not reflected in the surface topography, and probably predates the formation of the present canyon head.

Yukon River: Evidence for Extensive Migration during the Holocene Transgression.

The shift of the Yukon River, during the Holocene sea-level transgression, from south of Nunivak Island during the Wisconsin maximum to its present location (a distance greater than 300 kilometers) is indicated by remanent channels, distinct subbottom structures, deltaic sediments, and anomalous rates of sediment accumulation on the continental shelf of the east-central Bering Sea. These features were produced as the ancestral river migrated northward across the easternmost part of this area before 11,000 years ago.