ABSTRACT
This study presents the results of a number of serological tests, in particular the plate agglutination test using acid antigen stained with rose bengal, in the examination of 440 human sera for brucellosis. In addition, the sera were examined for the presence of specific antibodies of various classes (IgM and IgG). These investigations showed the rose bengal plate agglutination test to be highly specific and sensitive as a rapid method for the diagnosis of human brucellosis.
Subject(s)
Agglutination Tests/methods , Brucellosis/immunology , Rose Bengal , Antigens, Bacterial , Brucella/immunology , Coombs Test , Cysteine , Hemagglutination Tests , Humans , Immunoglobulin G/isolation & purification , Immunoglobulin M/isolation & purificationABSTRACT
This paper describes the results of many years' study of the indirect haemagglutination (IHA) test for brucellosis. It is shown that the use of sheep erythrocytes sensitized with a specific lipopolysaccharide antigen in the IHA test provides a specific method, which is more sensitive than the agglutination test, for the diagnosis of brucellosis in man and farm animals.
