ABSTRACT
The effect of the enzyme complex from Vobenzin on transfer of plasmid genes in biofilms of gramnegative bacteria was studied. The extracellular matrix of the bacterial biofilms was shown to contain extracellular DNA carrying the antibiotic resistance markers. The action of the enzyme complex resulted in lower frequency of the antibiotic resistance genes transfer in mixed bacterial biofilms.
Subject(s)
Biofilms/drug effects , Escherichia coli/drug effects , Genes, Bacterial , Hydrolases/pharmacology , Rutin/pharmacology , Ampicillin/pharmacology , Anti-Bacterial Agents/pharmacology , Conjugation, Genetic , Drug Combinations , Drug Resistance, Bacterial , Escherichia coli/genetics , Plasmids/genetics , Tetracycline/pharmacologySubject(s)
Enzymes, Immobilized , General Surgery/history , Peptide Hydrolases , Surgical Procedures, Operative , Wounds and Injuries , Bandages/history , Bandages/standards , Enzymes, Immobilized/administration & dosage , Enzymes, Immobilized/history , Enzymes, Immobilized/therapeutic use , History, 20th Century , History, 21st Century , Humans , Peptide Hydrolases/administration & dosage , Peptide Hydrolases/history , Peptide Hydrolases/therapeutic use , Russia , Surgical Procedures, Operative/methods , Surgical Procedures, Operative/trends , Wounds and Injuries/history , Wounds and Injuries/surgerySubject(s)
Bromelains/therapeutic use , Myocardial Infarction/drug therapy , Rutin/analogs & derivatives , Trypsin/therapeutic use , Ventricular Remodeling , Adult , Aged , Dilatation, Pathologic/physiopathology , Dilatation, Pathologic/prevention & control , Drug Combinations , Drug Therapy, Combination , Female , Humans , Male , Middle Aged , Myocardial Infarction/physiopathology , Rutin/therapeutic use , Treatment OutcomeABSTRACT
The clinical investigation included 48 patients with acute adhesive intestinal obstruction resulting from appendectomy and an analysis of the cytokine status in 34 children with acute phlegmonous appendicitis. The patients were divided into two groups: a "polyenzyme" group and a "monoenzyme" group (Hyaluronidase). In the "polyenzyme" group the children were given the preparation Wobenzym in addition to the complex therapy, in the "monoenzyme" group--electrophoresis of Hyaluronidase. In the "monoenzyme" group there were 2 cases of intestinal obstruction. The patients from the "polyenzyme" group had no postoperative complications. Wobenzym was shown to reduce (p < 0.05) the level of pro-inflammatory cytokines (IL-2, IL-6, TNF-alpha) and to increase the level of anti-inflammatory cytokine (IL-4). Using the polyenzyme preparations after abdominal operations in children was found to promote the reduction of intra-abdominal adhesion and inflammatory processes.
Subject(s)
Adjuvants, Immunologic/therapeutic use , Appendectomy/adverse effects , Hydrolases/therapeutic use , Peritoneal Diseases/prevention & control , Rutin/therapeutic use , Child , Drug Combinations , Female , Follow-Up Studies , Humans , Intestinal Obstruction/etiology , Intestinal Obstruction/prevention & control , Male , Peritoneal Diseases/complications , Postoperative Complications , Retrospective Studies , Tissue Adhesions/etiology , Tissue Adhesions/prevention & control , Treatment OutcomeABSTRACT
The impact of papain, trypsin and the enzyme complex vobenzyme on formation of biofilms by grampositive and gramnegative bacteria was studied. The enzymes were shown to inhibit the biofilm formation. When applilied to the formed associations, the enzymes potentiated the effect of antibiotics on the bacteria located in them. An increase in the antimicrobial effect of various nonrelated antibiotics was not connected with a change in the bacteria susceptibility but likely resulted from higher bioavailability of the drugs in the presence of the enzymes.
