Parentage influence on gene expression under acidification revealed through single-embryo sequencing.

The dissolution of anthropogenic carbon dioxide (CO2 ) in seawater has altered its carbonate chemistry in the process of ocean acidification (OA). OA affects the viability of marine species. In particular, calcifying organisms and their early planktonic larval stages are considered vulnerable. These organisms often utilize energy reserves for metabolism rather than growth and calcification as supported by bulk RNA-sequencing (RNA-seq) experiments. Yet, transcriptomic profiling of a bulk sample reflects the average gene expression of the population, neglecting the variations between individuals, which forms the basis for natural selection. Here, we used single-embryo RNA-seq on larval sea urchin Heliocidaris crassispina, which is a commercially and ecologically valuable species in East Asia, to document gene expression changes to OA at an individual and family level. Three paternal half-sibs groups were fertilized and exposed to 3 pH conditions (ambient pH 8.0, 7.7 and 7.4) for 12 h prior to sequencing and oxygen consumption assay. The resulting transcriptomic profile of all embryos can be distinguished into four clusters, with differences in gene expressions that govern biomineralization, cell differentiation and patterning, as well as metabolism. While these responses were influenced by pH conditions, the male identities also had an effect. Specifically, a regression model and goodness of fit tests indicated a significant interaction between sire and pH on the probability of embryo membership in different clusters of gene expression. The single-embryo RNA-seq approach is promising in climate stressor research because not only does it highlight potential impacts before phenotypic changes were observed, but it also highlights variations between individuals and lineages, thus enabling a better determination of evolutionary potential.

Cytosolic antibody receptor TRIM21 is required for effective tau immunotherapy in mouse models.

Aggregates of the protein tau are proposed to drive pathogenesis in neurodegenerative diseases. Tau can be targeted by using passively transferred antibodies (Abs), but the mechanisms of Ab protection are incompletely understood. In this work, we used a variety of cell and animal model systems and showed that the cytosolic Ab receptor and E3 ligase TRIM21 (T21) could play a role in Ab protection against tau pathology. Tau-Ab complexes were internalized to the cytosol of neurons, which enabled T21 engagement and protection against seeded aggregation. Ab-mediated protection against tau pathology was lost in mice that lacked T21. Thus, the cytosolic compartment provides a site of immunotherapeutic protection, which may help in the design of Ab-based therapies in neurodegenerative disease.

Local emergency management's use of social media during disasters: a case study of Hurricane Irma.

Governments' use of social media during all phases of emergency management, especially during disasters, has increased dramatically in the past 20 years. Yet, implementation at the local government level in the United States remains haphazard. As technology and the role of social media evolve, there persists a need to understand the socio-technical aspects of social media's employment in times of disaster. This study contributes to the growing social media literature by asking the following questions: what challenges remain and what lessons learned are being institutionalised at the local level of government? A qualitative analysis of 26 after action reports on Hurricane Irma (September 2017) by county, state, and federal governments and a four-hour focus-group session revealed dominant and subdominant themes, including: push/pull information; capacity and technical issues; inconsistent messaging; one-way versus two-way communication; timing of messages; and data collection. The paper concludes by discussing lessons learned, remaining challenges, evidence of organisational learning, and recommendations for future research.

Cholesterol determines the cytosolic entry and seeded aggregation of tau.

Assemblies of tau can transit between neurons, seeding aggregation in a prion-like manner. To accomplish this, tau must cross cell-limiting membranes, a process that is poorly understood. Here, we establish assays for the study of tau entry into the cytosol as a phenomenon distinct from uptake, in real time, and at physiological concentrations. The entry pathway of tau is cell type specific and, in neurons, highly sensitive to cholesterol. Depletion of the cholesterol transporter Niemann-Pick type C1 or extraction of membrane cholesterol renders neurons highly permissive to tau entry and potentiates seeding even at low levels of exogenous tau assemblies. Conversely, cholesterol supplementation reduces entry and almost completely blocks seeded aggregation. Our findings establish entry as a rate-limiting step to seeded aggregation and demonstrate that dysregulated cholesterol, a feature of several neurodegenerative diseases, potentiates tau aggregation by promoting entry of tau assemblies into the cell interior.

Disaster Recovery Communication in the Digital Era: Social Media and the 2016 Southern Louisiana Flood.

This study explores disaster recovery communication in the digital era. In particular, this study analyzes Twitter communication data corresponding to the 2016 Southern Louisiana flood recovery process and examines patterns and characteristics of long-term recovery communication. Based on network and sentiment analyses of the longitudinal Twitter data, the study identifies the dynamic changes in participants' numbers, dominant voices, and sentiments in social media communication during the long-term recovery process. From the additional content analysis of relevant news articles, in-depth contextual information is provided to support and supplement the findings. Findings show the weaning communication volume during the recovery phase, lacking local voices over the long-term recovery communication process, and prolonging negative sentiments over the recovery period. Based on the findings, the authors provide implications highlighting the need for investing in long-term recovery communication, better utilizing information from social media, and supporting local voices during disaster recovery.

Adding insult to injury: The fiscal impact of a failed FEMA disaster reimbursement system upon Florida municipalities.

The financial aspects of natural disasters test fiscal solvency by draining municipal reserves and diverting funds from vital operations until Federal Emergency Management Agency (FEMA) reimbursements arrive, if they arrive. With record-breaking natural disasters, the resulting fiscal strain is hampering nearly every community's effort to increase resiliency. Without systemically assessing the financial responses to natural disasters at the local government level, we are perpetuating the paradox of government disaster policy making and decreasing our community's resiliency. This study bridges the gap between the financial management and disaster recovery literatures by applying resource dependency theory to an exploratory case study of local emergency managers and city managers in Central Florida following hurricanes Matthew, Irma, and Michael. Collectively, the respondents describe the reactive and dependent nature of the current federalist approach to natural disaster financial management practices; which ultimately threatens fiscal viability for many American communities.

Tau assemblies do not behave like independently acting prion-like particles in mouse neural tissue.

A fundamental property of infectious agents is their particulate nature: infectivity arises from independently-acting particles rather than as a result of collective action. Assemblies of the protein tau can exhibit seeding behaviour, potentially underlying the apparent spread of tau aggregation in many neurodegenerative diseases. Here we ask whether tau assemblies share with classical pathogens the characteristic of particulate behaviour. We used organotypic hippocampal slice cultures from P301S tau transgenic mice in order to precisely control the concentration of extracellular tau assemblies in neural tissue. Whilst untreated slices displayed no overt signs of pathology, exposure to recombinant tau assemblies could result in the formation of intraneuronal, hyperphosphorylated tau structures. However, seeding ability of tau assemblies did not titrate in a one-hit manner in neural tissue. The results suggest that seeding behaviour of tau arises at high concentrations, with implications for the interpretation of high-dose intracranial challenge experiments and the possible contribution of seeded aggregation to human disease.

A stromal cell niche sustains ILC2-mediated type-2 conditioning in adipose tissue.

Group-2 innate lymphoid cells (ILC2), type-2 cytokines, and eosinophils have all been implicated in sustaining adipose tissue homeostasis. However, the interplay between the stroma and adipose-resident immune cells is less well understood. We identify that white adipose tissue-resident multipotent stromal cells (WAT-MSCs) can act as a reservoir for IL-33, especially after cell stress, but also provide additional signals for sustaining ILC2. Indeed, we demonstrate that WAT-MSCs also support ICAM-1-mediated proliferation and activation of LFA-1-expressing ILC2s. Consequently, ILC2-derived IL-4 and IL-13 feed back to induce eotaxin secretion from WAT-MSCs, supporting eosinophil recruitment. Thus, MSCs provide a niche for multifaceted dialogue with ILC2 to sustain a type-2 immune environment in WAT.

The scholarship of teaching and learning in emergency management and homeland security: Trends, gaps, barriers, and opportunities.

Underlying the nature of what is and is not a profession are education standards. Among the characteristics defining a profession's specialized boundaries are the education standards comprising its academic programs. The status of the Scholarship of Teaching and Learning (SoTL) is explored for both emergency management and homeland security (EM/HS) at this point in time. The aim of this article is to increase the conceptual understanding of SoTL by discussing what SoTL is, explaining why it is important, exploring our current state of knowledge in this area, and by identifying current barriers and opportunities for improvement. Several possible means of better integrating SoTL into the EM/HS discipines are highlighted: Making SoTL a higher priority, increasing exposure to existing research, advancing knowledge within our own disciplines, sharing knowledge through publications and conferences, and applying knowledge in our teaching. Conclusively, the vital nature of SoTL research can more deeply establish the professional boundaries for both disciplines.

Advancing emergency management higher education: Importance of cultural competence scholarship.

The evolving demographics, needs, and demands of society requires managers to be culturally competent in all phases of emergency management, especially when responding to disasters. A culturally incompetent manager and/or organization can increase a hazard's impact on often already vulnerable communities. Are cultural competencies lacking in emergency management education? Are these competencies as important as other highly regarded emergency management competencies? What are effective pedagogy and andragogy to create, implement, and evaluate cultural competency in emergency management higher education? As scholars work to advance the emergency management discipline, there is an increasing need for scholarship of teaching and learning research, especially regarding cultural competency. This article reviews the existing literature, as well as pedagogy from related disciplines, to identify gaps and provide recommendations for future research.

Unveiling cultures in emergency response communication networks on social media: following the 2016 Louisiana floods.

While culture in emergency management has gained attention from the field of risk communication, few have systemically dealt with the nuances of general culture involved in the formation and differentiation of risk communication. To fill this gap, this research aims to first examine cultural nuances from the 2016 Louisiana flood response by primarily focusing on communications embedded in social media. The results from social network analysis and content analysis highlight that the flood response communication had strong cultural characteristics, highlighting the notion that of the cultures in Louisiana-faith-based, local authority, and nonprofits-were the prominent cultural responders in the flood response communication. In particular, cultural similarity in both intra/inter group response communication was observed, with each communication group comprising actors who shared a common cultural background and spoke similar keywords.

Evolution of an experiential learning partnership in emergency management higher education.

Experiential learning allows students to step outside the classroom and into a community setting to integrate theory with practice, while allowing the community partner to reach goals or address needs within their organization. Emergency Management and Homeland Security scholars recognize the importance, and support the increased implementation, of this pedagogical method in the higher education curriculum. Yet challenges to successful implementation exist including limited resources and time. This longitudinal study extends the literature by detailing the evolution of a partnership between a university and office of emergency management in which a functional exercise is strategically integrated into an undergraduate course. The manuscript concludes with a discussion of lessons learned from throughout the multiyear process.

Analyzing after-action reports from Hurricanes Andrew and Katrina: repeated, modified, and newly created recommendations.

Thirteen years after Hurricane Andrew struck Homestead, FL, Hurricane Katrina devastated the Gulf Coast of Mississippi, Alabama, and southeastern Louisiana. Along with all its destruction, the term "catastrophic" was redefined. This article extends the literature on these hurricanes by providing a macrolevel analysis of The Governor's Disaster Planning and Response Review Committee Final Report from Hurricane Andrew and three federal after-action reports from Hurricane Katrina, as well as a cursory review of relevant literature. Results provide evidence that previous lessons have not been learned or institutionalized with many recommendations being repeated or modified. This article concludes with a discussion of these lessons, as well as new issues arising during Hurricane Katrina.