1.
Anal Biochem
; 150(2): 442-8, 1985 Nov 01.
Article
in English
| MEDLINE
| ID: mdl-4091268
ABSTRACT
We describe two methods that facilitate detection and characterization of DNA methyltransferases: activity gel analysis and the use of DNA-cellulose or DNA-Sepharose in DNA methylation reactions. The first permits identification of catalytic subunits, determination of the influence of proteolysis, and evolutionary or developmental studies. The second allows accurate and fast determination of DNA methyltransferase activities in crude extracts and during purification.
Subject(s)
DNA (Cytosine-5-)-Methyltransferases/analysis , Catalysis , Cellulose/analogs & derivatives , DNA/analogs & derivatives , DNA, Bacterial , DNA, Single-Stranded , Electrophoresis, Polyacrylamide Gel , Methylation , Micrococcus/genetics , Photofluorography , Sepharose
2.
Prog Clin Biol Res
; 198: 133-55, 1985.
Article
in English
| MEDLINE
| ID: mdl-2999811
