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Fiziol Cheloveka ; 41(2): 109-12, 2015.
Article in Russian | MEDLINE | ID: mdl-26027341

ABSTRACT

A new method of preservation of nucleated cells in the electric refrigerator with xenon. After slow freezing and storage is even one day at -80 °C persists for more than 60% leukocytes. Cell membranes are resistant to the vital dye. In 85% of granulocytes stored baseline lysosomal-cationic protein, reduced lipid peroxidation and antioxidant activity. Cryopreservation of biological objects in inert gases is a promising direction in the practice of medicine and can be an alternative to the traditional method using liquid nitrogen.


Subject(s)
Blood Preservation/methods , Cryopreservation/methods , Granulocytes/cytology , Granulocytes/metabolism , Xenon/chemistry , Antioxidants/metabolism , Humans , Lipid Peroxidation , Lysosomes/metabolism
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