ABSTRACT
A new strain of Microtetraspora parvosata subsp. kistnae subsp. nov. (ATCC 55076) was found to produce new antiviral antibiotics, designated kistamicins A and B. These antibiotics exhibited activity against influenza virus type A and moderate activity against Gram-positive bacteria.
Subject(s)
Actinomycetales/chemistry , Anti-Bacterial Agents/isolation & purification , Anti-Bacterial Agents/pharmacology , Antiviral Agents/isolation & purification , Antiviral Agents/pharmacology , Bacterial Proteins/isolation & purification , Bacterial Proteins/pharmacology , Peptides , Actinomycetales/classification , Actinomycetales/metabolism , Animals , Anti-Bacterial Agents/biosynthesis , Anti-Bacterial Agents/chemistry , Antiviral Agents/metabolism , Bacterial Proteins/chemistry , Chemical Phenomena , Chemistry, Physical , Dogs , Fermentation , HeLa Cells , Herpes Simplex/drug therapy , Humans , Influenza A virus/drug effects , Influenza, Human/drug therapy , Magnetic Resonance Spectroscopy , Simplexvirus/drug effects , Vero CellsSubject(s)
Antibiotics, Antineoplastic/isolation & purification , Antifungal Agents/isolation & purification , Animals , Antibiotics, Antineoplastic/chemistry , Antibiotics, Antineoplastic/pharmacology , Antifungal Agents/chemistry , Antifungal Agents/pharmacology , Butadienes/chemistry , Butadienes/isolation & purification , Butadienes/pharmacology , Fermentation , Humans , Melanoma, Experimental/drug therapy , Mice , Microbial Sensitivity Tests , Nitriles/chemistry , Nitriles/isolation & purification , Nitriles/pharmacology , Tumor Cells, Cultured/drug effectsABSTRACT
BMS-181184 is a new semisynthetic pradimicin derivative with a broad-spectrum antifungal activity. In a search for actinomycetes producing BMS-181184, 4 strains of Actinomadura sp. isolated from soil samples were found to produce the antibiotic under conditions of directed biosynthesis. Among them, Actinomadura sp. AB1236 proved most useful in the production of BMS-181184 when fermented in a medium containing D-serine and D-cycloserine. A minor product isolated from the broth of strain AB1236 was identified as the dexylosyl analog of BMS-181184, which was also obtained by acid hydrolysis of BMS-181184.
Subject(s)
Actinomycetales/metabolism , Anthracyclines , Antibiotics, Antineoplastic/biosynthesis , Antifungal Agents/biosynthesis , Actinomycetales/classification , Antibiotics, Antineoplastic/isolation & purification , Antibiotics, Antineoplastic/pharmacology , Antifungal Agents/isolation & purification , Antifungal Agents/pharmacology , Fungi/drug effectsABSTRACT
New antibiotic pumilacidins A, B, C, D, E, F and G were isolated from the culture broth of a strain of Bacillus pumilus. They are cyclic acylheptapeptide composed of a beta-hydroxy fatty acid, two L-leucine, two D-leucine, L-glutamic acid, L-aspartic acid and L-isoleucine (or L-valine). Pumilacidin components were inhibitory to herpes simplex virus type 1 and H+, K(+)-ATPase and demonstrated antiulcer activity in rat.
Subject(s)
Anti-Bacterial Agents , Anti-Bacterial Agents/isolation & purification , Antiviral Agents/isolation & purification , Bacillus/metabolism , Peptides, Cyclic/isolation & purification , Peptides , Simplexvirus/drug effects , Adenosine Triphosphatases/antagonists & inhibitors , Amino Acid Sequence , Animals , Anti-Bacterial Agents/analysis , Anti-Bacterial Agents/pharmacology , Anti-Bacterial Agents/therapeutic use , Antiviral Agents/analysis , Antiviral Agents/pharmacology , Antiviral Agents/therapeutic use , Bacillus/classification , Chromatography , Disease Models, Animal , Fermentation , H(+)-K(+)-Exchanging ATPase , Magnetic Resonance Spectroscopy , Male , Molecular Sequence Data , Peptides, Cyclic/analysis , Peptides, Cyclic/pharmacology , Peptides, Cyclic/therapeutic use , Rats , Rats, Inbred Strains , Spectrophotometry, Infrared , Stomach Ulcer/prevention & control , Vero CellsSubject(s)
Bacillus/growth & development , Dipeptides/isolation & purification , Leucyl Aminopeptidase/antagonists & inhibitors , Animals , Bacillus/classification , Chemical Phenomena , Chemistry , Culture Media , Dipeptides/pharmacology , Kidney/enzymology , Kinetics , Magnetic Resonance Spectroscopy , Mass Spectrometry , Rabbits , Spectrophotometry, Ultraviolet , Substrate Specificity , SwineABSTRACT
An unidentified oligosporic actinomycete strain, No. E864-61, produced two new antibiotics, Bu-2313 A (C27H35NO9) and Bu-2313 B (C26H33NO9). Bu-2313 A and B each exhibited a broad antibiotic spectrum against Gram-positive and Gram-negative anaerobic bacteria, and showed in vivo activity against experimental infections produced by B. fragilis and C. perfringens. Bu-2313 also inhibited some aerobic bacteria such as streptococci. Bu-2313 B was approximately two-fold more active than Bu-2313 A.
