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1.
J Vet Med Sci ; 78(5): 747-54, 2016 Jun 01.
Article in English | MEDLINE | ID: mdl-26781707

ABSTRACT

The first milk substitute for giant panda cubs was developed in 1988 based on limited data about giant panda breast milk and that of certain types of bear. Mixtures of other formulas have also been fed to cubs at some facilities. However, they are not of sufficient nutritional quality for promoting growth in panda cubs. Here, we report analysis of giant panda breast milk and propose new milk substitutes for cubs, which were developed based on the results of our analysis. The Chengdu Research Base of Giant Panda Breeding obtained breast milk samples from three giant pandas. Up to 30 ml of breast milk were collected from each mother by hand. Then, the milk samples were frozen and sent to Nihon University. The levels of protein, fat, carbohydrates, ash, moisture, vitamins, minerals, total amino acids, fatty acids, lactose and other carbohydrates in the milk were analyzed. The breast milk samples exhibited the following nutritional values: protein: 6.6-8.5%, fat: 6.9-16.4%, carbohydrates: 2.5-9.1%, ash: 0.9-1.0% and moisture: 67-83%. We designed two kinds of milk substitutes based on the data obtained and the nutritional requirements of dogs, cats and rodents. The nutritional composition of the milk substitutes for the first and second stages was as follows: protein: 38 and 26%, fat: 40 and 40%, carbohydrates: 13 and 25%, ash: 6 and 6% and moisture: 3 and 3%, respectively. In addition, the substitutes contained vitamins, minerals, taurine, docosahexaenoic acid, lactoferrin, nucleotides and other nutrients.


Subject(s)
Animal Feed , Milk Substitutes , Milk/chemistry , Ursidae , Amino Acids/analysis , Animals , Carbohydrates/analysis , Fatty Acids/analysis , Female , Lactose/analysis , Milk Proteins/analysis , Vitamins/analysis
2.
J Vet Med Sci ; 77(4): 503-6, 2015 Apr.
Article in English | MEDLINE | ID: mdl-25649521

ABSTRACT

We examined the effects of chicken egg hydrolysate (also known as "bone peptide" or BP) on bone metabolism in 5- to 8-month-old orchidectomized dogs. The bone formation marker serum bone alkaline phosphatase (BAP) and the bone resorption marker urine deoxypyridinoline (DPD) were used as indicators to measure changes in bone metabolism. The following results were observed that Serum BAP was higher in dogs fed BP-enriched food throughout the clinical investigation. Serum BAP was statistically significantly higher in dogs fed BP-enriched food than in dogs fed non-BP-enriched food at 2 months after orchidectomy. This suggests that BP promoted bone formation immediately after orchidectomy.


Subject(s)
Animal Feed/analysis , Bone and Bones/metabolism , Egg Yolk/chemistry , Orchiectomy/veterinary , Protein Hydrolysates/pharmacology , Animals , Diet/veterinary , Dietary Supplements , Dogs , Male , Protein Hydrolysates/chemistry
3.
J Vet Med Sci ; 76(3): 439-46, 2014 Mar.
Article in English | MEDLINE | ID: mdl-24212506

ABSTRACT

In small animal veterinary practices, body condition score (BCS) is generally used to diagnose obesity. However, BCS does not constitute objective data. In this study, we investigated the value of using human body fat analysis software for male dogs. We also compared changes in body fat after neutering. Changes in body fat at the time of neutering (age 1 year) and 1 year later were compared by performing CT scanning and using human body fat analysis software. We found that body fat increased in all the individuals tested. In terms of the site of fat accumulation, subcutaneous fat was more pronounced than visceral fat with a marked increase on the dorsal side of the abdomen rather than the thorax.


Subject(s)
Body Composition/physiology , Castration/veterinary , Software , Age Factors , Animals , Dogs , Male , Tomography, X-Ray Computed/methods , Tomography, X-Ray Computed/veterinary
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