1.
Dokl Akad Nauk SSSR
; 307(1): 226-8, 1989.
Article
in Russian
| MEDLINE
| ID: mdl-2806061
2.
Bioorg Khim
; 14(1): 43-7, 1988 Jan.
Article
in Russian
| MEDLINE
| ID: mdl-3382431
ABSTRACT
A simple and economy method of the biochemical assembling of long double-stranded DNA segments is described. A single-stranded polydeoxynucleotide 122 bases long representing a fragment of synthetic gene of human beta-interferon was assembled from three synthetic fragments 36 (two) and 50 bases long on four complementary 12-mers as templates. This single-stranded polynucleotide was converted, in the presence of DNA polymerase 1 and a 12-meric primer, in to the full-length double-stranded DNA (the beta-interferon gene segment). It was cloned into an E. coli plasmid vector pBR322 and its sequence confirmed.