[Characteristics of the ionic composition and ultrastructure of Bordetella pertussis in controlled regulation of the mineral element content in a growth medium]. / Osobennosti ionnogo sostava i ul'trastruktury Bordetella pertussis v usloviiakh napravlennoi reguliatsii soderzhaniia nekotorykh mineral'nykh élementov v srede vyrashchivaniia.

The content of trace elements necessary for the normal growth of bacteria was found to have no effect on the intracellular concentration of Ca2+ and Al3+. The content of Cu2+, Fe3+, Mn2+, Mg2+ was considerably reduced. The addition of Mg2+ at different concentrations to this culture medium stimulated the capacity of cells for accumulating not only Mg2+, but also some other ions. Their maximum intracellular concentration was observed when the concentration of Mg2+ in the culture medium was 41 mM. The growth of microbial cells in the standard culture medium containing Mg2+ at a concentration of 4 mM was accompanied by the increased consumption of elements actively participating in redox reactions (Cu2+, Fe3+, Mn2+). Shifts in the ionic composition of microbial cells were manifested by the morphological features of B. pertussis, linked with the increased synthesis of crystalloid structures. The influence of Mn2+, Al3+, Zn2+ at different concentrations on the ionic composition and morphology of B. pertussis was studied.

[Metal content in Bacillus megaterium cells under different cultivation conditions]. / Soderzhanie metallov v kletkakh Bacillus megaterium pri razlichnykh usloviiakh kul'tivirovaniia.

The content of K, Na, Mg, Fe, Al, Ba, Sr and Cu was studied in the cells of a chemostal culture of Bacillus megaterium whose growth was limited with citrate, the growth rates being D=mu=0.2, 0.4 and 0.7 hr(-1). The growth was affected also by acid and alkaline values of pH. The content of metals in the cells could change tenfold, depending on their physiological state. The content of Ca, Mg and Fe increased with the growth rate while that of K remained constant. The content of metals changed even more sharply, depending on pH. In the alkaline medium, the content of K, Na, Mg, Ca and Fe decreased, in contrast to that of Al, Ba, Sr and Cu, probably as a result of damaged transport.

[Study of the relationship between the trace element content and the physiological properties of Salmonella typhi during submerged cultivation]. / Izuchenie vzaimosviazi mezhdu soderzhaniem mikroelementov i fiziologicheskimi svoistvami S. typhi v protsesse glubinnogo kul'tivirovaniia.

The content of trace elements in the cells served as one of the characteristics of physiological condition of the population. The character of the changes in the trace elements content depending on the rate of the microbial multiplication pointed to the importance of the mineral sources of nutrition for the development of the cells analyzed.

[Trace element content in comparison with the physiological and immunogenic properties of S. typhi obtained by various continuous cultivation regimens]. / Soderzhanie mikroélementov v sopostavlenii s fiziologicheskimi i immunogennymi svoistvami S. typhi, poluchennykh pri razlichnykh rezhimakh nepreryvnogo kul'tivirovaniia.

The content of trace elements in the S. typhi cells was studied during continuous cultivation in the synthetic nutrient medium. Growing under conditions of complete or incomplete glucose utilization failed to influence the content of trace elements in these cells. The same tendencies were revealed in the changes of manganese, iron, magnesium, calcium, and strontium content with increase of the growth rate. The content of aluminium in the cells altered in parallel with the changes in the immunogenic properties with increase of the growth rate from 0.17 to 0.44 hour-1.