ABSTRACT
Within the framework of the III All-Russian scientific and practical conference of otorhinolaryngologists, audiologists, somnologists and maxillofacial surgeons with international participation, a meeting of experts in the field of ENT diseases was held with the support of Heel RUS LLC, October 17-18, 2019, Kazan.
Subject(s)
Otorhinolaryngologic Diseases , Audiologists , Humans , RussiaABSTRACT
The article deals with the results of study targeted to develop polymer diagnostic preparation to identify epidemically significant serogroups Legionella pneumophilia. The preparation combines rate of record (1-5 min) of reaction of paragglutinining preparations with color visualization and demonstrative of reaction of volume agglomeration with polymer diagnosticums. The specially synthesized polymer microspheres were sensibilized with serums enriched with antibodies to lipopolysaccharide of corresponding serovar L. pneumophilia. The derived immunoglobulin diagnostic preparations detect agent of legionellesis in the reaction of slide-agglutination on glass during 1-5 min. The polymer diagnostic preparations provide positive reaction with culture of corresponding serovar and no reaction with other gomologic and geterologic agents of infectious diseases.
Subject(s)
Immunoglobulins , Legionella pneumophila/isolation & purification , Legionellosis/diagnosis , Lipopolysaccharides/isolation & purification , Polymers , Serotyping , Agglutination/immunology , Humans , Immunoglobulins/chemistry , Immunoglobulins/immunology , Legionella pneumophila/chemistry , Legionella pneumophila/immunology , Legionellosis/immunology , Legionellosis/microbiology , Lipopolysaccharides/chemistry , Lipopolysaccharides/immunology , Polymers/chemical synthesisABSTRACT
The objective of this work was to study the feasibility and effectiveness of the cavitational low-frequency ultrasound treatment of vasomotor rhinitis in combination with the application of topical corticosteroids. The study included 220 patients with the confirmed neurovegetative form of vasomotor rhinitis treated with the use of a Kavitar apparatus for the ultrasonic cavitational jet lavage. The data obtained indicate that the low-frequency cavitational treatment of intranasal mucosa in combination with the application of topical corticosteroids may be recommended as an efficatious method for the treatment of vasomotor rhinitis.
Subject(s)
Adrenal Cortex Hormones/administration & dosage , Nasal Mucosa/drug effects , Rhinitis, Vasomotor/therapy , Ultrasonic Therapy/methods , Administration, Intranasal/methods , Administration, Topical , Adult , Combined Modality Therapy , Female , Humans , Male , Middle Aged , Nasal Lavage/methods , Nasal Mucosa/physiopathology , Rhinitis, Vasomotor/physiopathology , Treatment OutcomeABSTRACT
Technical approaches to construction of preparations for serologic diagnostics of Legionella infection were presented in the article; antigenic- and immunoglobulin-based diagnostic kits with known characteristics were developed. Immunogenic properties of protein and lypopolysaccharide antigens, which have diagnostic value, were studied; similarity of protein antigens from 7 serogroups of L. pneumophila was demonstrated. Soluble antigen with known composition was obtained and used for the development of antigen-based polymeric kit for diagnostics of Legionella infection. On the basis of hyperimmune sera, immunoglobulin-based polymeric diagnostic kit and array of coagglutinating diagnostic kits for the mentioned 7 serogroups were developed. Antigen-based polymeric diagnostic kit was recommended for licensure.
Subject(s)
Antibodies, Bacterial/immunology , Bacteriological Techniques/methods , Legionella/immunology , Legionellosis/diagnosis , Agglutination , Animals , Antibody Specificity , Antigens, Bacterial/analysis , Antigens, Bacterial/immunology , Bacterial Outer Membrane Proteins/immunology , Humans , Immune Sera , Immunoenzyme Techniques , Immunoglobulins , Legionellosis/microbiology , Legionellosis/urine , Lipopolysaccharides/analysis , Lipopolysaccharides/immunology , Microspheres , Polymers , Rabbits , Reagent Kits, Diagnostic/microbiology , Sensitivity and SpecificityABSTRACT
Study showed that El-Tor strains of V. cholerae isolated from different sources produce lipase for hemolysis after cultivation during 24 h on meat-peptone broth independently from their toxigenic and hemolytic abilities. Study of 3- and 4-hours broth cultures of vibrios revealed possibility to differentiate between hemolytic nontoxigenic strains and toxigenic nonhemolytic ones. Using antilipaze diagnostic kit it was possible to differentiate El-Tor vibrios from vibrios of classic biovar basing on lipase production 24 h after cultivation on meat-peptone broth that was evident in El-Tor vibrios but not in classic biovar strains.
Subject(s)
Cholera/diagnosis , Lipase/analysis , Vibrio cholerae O1/enzymology , Cholera/epidemiology , Culture Media , Diagnosis, Differential , Disease Outbreaks , Humans , Lipase/biosynthesis , Lipase/immunology , Reagent Kits, Diagnostic , Russia , Sensitivity and Specificity , Time Factors , Uzbekistan/epidemiology , Vibrio cholerae O1/classification , Vibrio cholerae O1/growth & developmentABSTRACT
On the basis of literature data and personal investigations the authors elaborated a scheme of detection of the causative agent of melioidosis in the objects of external environment, including fluorescent microscopy of crude material, seeding on meat-peptone agar with crystal violet, seeding material treated with penicillin on fluid medium with subsequent transfer on solid Levin medium and infection of animals, seeding on a medium with beta-alanine and neonmycin and infection of sensitive animals with crude material.
