ABSTRACT
PV-88 is the Ca2+ L channel blocker from pyrrole compounds, that has been used at hemorrhagic shock - HS (model of Shock was created by the method of (Wiggers-Feine) on 60th minute (intravenous administration, 3 mg/kg). Mean arterial pressure was 40 mmHg. Effects were studied on 80th minute after blood letting. Blood was exfused 40 ml/kg. Effect of PV-88 on redox processes in liver was studied. Experiments were carried out on male cats. Right lobe of the liver and the metabolic indices: ATP, CO, NAD, NADP, ALP, SDH, LDH were studied with the use of histochemistry (Pearse). Investigations have shown that on 80th minute after PV-88 administration CO (p<0.05), NADP (p<0.05), ALP (p<0.001), SDH (p<0.05), and LDH (p>0.05) were increased, while ATP (p<0.001) and NAD (p<0.001) were decreased. Increase in LDH was not statistically significant. According to the results of investigations, after administration of PV-88 at HS 60 on 80th minute, in liver increases oxygen concentration and aerobic process respectively.
Subject(s)
Blood Pressure/drug effects , Calcium Channel Blockers/pharmacology , Shock, Hemorrhagic/metabolism , Adenosine Triphosphate/metabolism , Animals , Calcium Channel Blockers/administration & dosage , Cats , Cytochromes/metabolism , L-Lactate Dehydrogenase/metabolism , Liver/drug effects , Liver/enzymology , Male , Models, Animal , NAD/metabolism , NADP/metabolism , Oxidation-Reduction , Oxygen/metabolism , Time FactorsABSTRACT
The aim of the research was to investigate electron-microscopic peculiarities of heart at different levels of antigen loading using adult and old aged gnotobiont rats, conventional rats and rats with experimental autoimmune cardiomyopathy. The results of investigations showed that the ultrasrtuctural characteristics of heart greatly depend on microbiological status and age of experimental animals. The ultrasrtucture of heart of adult rats mostly depend on the level of intensity of antigen loading. In all experimental groups of old rats the subcellular differences had the same directions. Although, it must be mentioned that there was not significant correlation between the level of heart damage and intensity of antigen loading. According to this, age-related changes depend not only on microbiological status of organism, but on genetically determined peculiarities of internal regulative processes taking place during the ontogenesis. Thus, the received results may be used to identify age-related changes, which give us the possibility for the differentiation between age-related and pathological peculiarities.
