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Onderstepoort J Vet Res ; 55(4): 227-9, 1988 Dec.
Article in English | MEDLINE | ID: mdl-3217095

ABSTRACT

DNA from the Washington, South-Idaho, Virginia and Florida isolates of Anaplasma marginale was hybridized to probes specific for Anaplasma centrale and A. marginale. The A. centrale probes AC-2 and AC-4 hybridized to identical bands on all of these isolates. The hybridization patterns suggests that the Virginia, Florida and the South African isolates are similar. A number of bands were obtained with the Washington isolate which differed from those obtained with the other isolates. Probe AC-2 could be developed to identify relatedness among Anaplasma isolates. Probe AC-2 detected A. marginale DNA in midgut material from infected Dermacentor andersoni ticks. No hybridization was obtained with DNA from salivary gland tissues from these infected ticks.


Subject(s)
Anaplasma/isolation & purification , DNA Probes , DNA, Bacterial , Dermacentor/microbiology , Nucleic Acid Hybridization , Ticks/microbiology , Anaplasma/genetics , Animals
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