Subject(s)
Anti-Bacterial Agents/administration & dosage , Anti-Bacterial Agents/pharmacokinetics , Penicillin G/administration & dosage , Penicillin G/pharmacokinetics , Animals , Anti-Bacterial Agents/blood , Dose-Response Relationship, Drug , Injections, Intramuscular/veterinary , Male , Penicillin G/blood , RabbitsABSTRACT
Toxic activity of 89 culture filtrates of Bacillus cereus strains were examined. From the total number of the filtrates tested, 3 were found to cause fluid accumulation in the ligated rabbit ileal loop. 71 samples caused a vascular permeability increase in the rabbit skin corresponding to an intermediate or strong toxin production. 59 of the 89 culture filtrates were cytotoxic on Vero cells, 31 had a lethal effect, 81 induced hemolysis of 0.5% rabbit erythrocytes and 55 tested samples showed lecithinase activity. The results showed that relationships exist between the extent of the permeability reaction and cytotoxicity, lecithinase and lethality. No relationship was found between permeability reaction size and reaction in the ligated rabbit ileal loop, nor a strict correlation between permeability reaction and hemolysin. Similarly, the part of culture filtrates showing a weak, or no reaction in the rabbit skin showed hemolytic activity.
Subject(s)
Bacillus cereus/metabolism , Bacterial Toxins/biosynthesis , Food Microbiology , Animals , Animals, Suckling , Bacillus cereus/isolation & purification , Bacterial Toxins/toxicity , Chlorocebus aethiops , Egg Yolk/drug effects , Hemolysis , Ileum/drug effects , Mice , Permeability/drug effects , Phospholipases/metabolism , Rabbits , Skin/drug effects , Vero CellsABSTRACT
Investigation of 367 P. aeruginosa strains primarily isolated from clinical and other biological material as well as from the environment yielded results suggesting a substantial toxinogenic potential. 92.6% of the assayed culture filtrates derived from the strains under investigation proved positive in the early skin tests on rabbits. 49.7% of the assayed material induced cytotoxic alterations on Vero cells, the rates for Y1 and CHO cells being 50.3% and 43.5% respectively. 54.3% culture filtrates caused haemolysis of rabbit RBC and 52.7% lysed horse RBC. Gelatinase activity was found in 96.3% of tested material, protease in 89.8%, lecithinase in 62.4% and elastase in 29.6%. 12.6% of tested material induced fluid accumulation in a ligated intestinal loop. None of the culture filtrates elicited a positive reaction in the suckling mice test suggesting the absence of the thermostable enterotoxin.
