ABSTRACT
Newcastle disease virus (NDV) is a paramyxovirus that bears two envelope glycoproteins at the virion surface. These proteins, fusion and hemagglutinin-neuraminidase (HN), are involved in the immune response against NDV infection. Recombinant cells constitutively expressing at their surface the HN protein from the velogenic Texas strain were generated by introducing the HN gene with a helper-free AEV-based vector. These recombinant cells were used to immunize chickens by various protocols, and birds were subsequently challenged with a lethal NDV injection. Both NDV protection and serologic response were observed.
Subject(s)
HN Protein/immunology , Newcastle Disease/prevention & control , Newcastle disease virus/immunology , Viral Vaccines/immunology , Animals , Avian Leukosis Virus/genetics , Avian Leukosis Virus/immunology , Cell Line , Chick Embryo , Genetic Vectors/genetics , Genetic Vectors/immunology , HN Protein/genetics , Kinetics , Newcastle disease virus/genetics , Recombinant Proteins/genetics , Recombinant Proteins/immunology , Vaccines, Synthetic/immunologyABSTRACT
Se trata de un estudio de prevalencia del universo de las defunciones por cáncer bucofaríngeo durante el sexenio 1980-1985, para determinar la tendencia de esta patología en el país. Para este propósito se revisaron todos los certificados de defunción del país separando los diagnósticos de cáncer bucofaríngeo (140-149 de la CIE). Los resultados confirmaron la hipótesis, observando un aumento del riesgo del 10,6%. La tasa más alta se observa en el hombre. Por edad, el 90% de las defunciones se produce en el mayor de 45 años. La localización más frecuente es la de lengua
Subject(s)
Adolescent , Adult , Middle Aged , Humans , Male , Female , Mouth Neoplasms/mortality , Pharyngeal Neoplasms/mortality , ChileABSTRACT
During the study of the DNA from 25 beta-thalassemic subjects from mediterranean origin the polymorphic Taq I restriction site located 3-kb 5' to the human delta-globin gene, was found non-randomly associated to the polymorphic Hind III sites within the G gamma- and A gamma-globin genes. This indicates that the 3' limit of the linkage group of polymorphic restriction sites including the gamma-globin genes is located downstream to the polymorphic Taq I site.
Subject(s)
DNA Restriction Enzymes , DNA/genetics , Deoxyribonucleases, Type II Site-Specific , Globins/genetics , Polymorphism, Genetic , Thalassemia/genetics , Base Sequence , Deoxyribonuclease HindIII , Heterozygote , Humans , Nucleic Acid HybridizationABSTRACT
By using Hph I and Rsa I restriction enzymes and beta globin large intervening sequence as a probe, we have investigated the DNA of 20 Algerian patients with beta(0) or beta(+) thalassemia. In any of them, we detected the nucleotide change which is known to generate an additional Hph I site at the 5' splice junction of the beta globin large intervening sequence and which yields a beta(0) phenotype. In one of them, we detected the nucleotide change which is known to generate an additional Rsa I site within the beta globin large intervening sequence and which is supposed to yield a beta(+) phenotype. These results indicate that these two types of mutation are relatively rare in the Algerian population.
Subject(s)
DNA Restriction Enzymes/metabolism , DNA/analysis , Deoxyribonucleases, Type II Site-Specific , Thalassemia/genetics , Base Sequence , Globins/genetics , HumansABSTRACT
Mapping of the DNA from 14 Mediterranean subjects indicates a genetic variation in an Hinf I recognition site located 1 kilobase 5' to the beta-globin gene. This Hinf I site was found associated with eight beta-thalassemic genes and 11 normal beta genes, and hence is not specifically linked to beta-thalassemia.
