ABSTRACT
A polyphasic analysis was undertaken of seven independent isolates of gram-negative cocci collected from pathological clinical samples from New York, Louisiana, Florida and Illinois and healthy subgingival plaque from a patient in Virginia, USA. The 16S rRNA gene sequence similarity among these isolates was 99.7-100â%, and the closest species with a validly published name was Neisseria lactamica (96.9â% similarity to the type strain). DNA-DNA hybridization confirmed that these isolates are of the same species and are distinct from their nearest phylogenetic neighbour, N. lactamica. Phylogenetic analysis of 16S and 23S rRNA gene sequences indicated that the novel species belongs in the genus Neisseria. The predominant cellular fatty acids were C16â:â0, summed feature 3 (C16â:â1ω7c and/or iso-C15â:â0 2-OH) and C18â:â1ω7c. The cellular fatty acid profile, together with other phenotypic characters, further supports the inclusion of the novel species in the genus Neisseria. The name Neisseria oralis sp. nov. (type strain 6332(T) â=âDSM 25276(T) â=âLMG 26725(T)) is proposed.
Subject(s)
Dental Plaque/microbiology , Gingiva/microbiology , Neisseria/classification , Phylogeny , Bacterial Typing Techniques , DNA, Bacterial/genetics , Fatty Acids/analysis , Humans , Molecular Sequence Data , Neisseria/genetics , Neisseria/isolation & purification , Nucleic Acid Hybridization , RNA, Ribosomal, 16S/genetics , RNA, Ribosomal, 23S/genetics , Sequence Analysis, DNA , United StatesABSTRACT
An analysis of 16S rRNA gene sequences from archived clinical reference specimens identified a novel species of the genus Psychrobacter, of which four strains have been independently isolated from human blood. On the basis of 16S rRNA gene sequence similarity, the closest relatives with validly published names were Psychrobacter arenosus R7(T) (98.7%), P. pulmonis CECT 5989(T) (97.7%), P. faecalis Iso-46(T) (97.6%) and P. lutiphocae IMMIB L-1110(T) (97.2%). Maximum-likelihood phylogenetic analysis of 16S rRNA gene sequences showed that the isolates belonged to the genus Psychrobacter and were members of a cluster associated with Psychrobacter sp. PRwf-1, isolated from a silk snapper fish. DNA-DNA relatedness and partial 23S rRNA gene sequences also supported the finding that the isolates belonged to a species distinct from its closest phylogenetic neighbours. The predominant cellular fatty acids were C(18:1)ω9c, C(16:0), summed feature 3 (C(16:1)ω7c and/or iso-C(15:0) 2-OH), summed feature 5 (C(18:2)ω6,9c and/or anteiso-C(18:0)) and C(18:0). Biochemical and morphological analysis further supported the assignment of the four isolates to a novel species. The name Psychrobacter sanguinis sp. nov. is proposed. The type strain is 13983(T) (=DSM 23635(T)=CCUG 59771(T)).
Subject(s)
Moraxellaceae Infections/microbiology , Psychrobacter/classification , Psychrobacter/isolation & purification , Bacteremia/microbiology , Bacterial Typing Techniques , Blood/microbiology , Cluster Analysis , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , Fatty Acids/analysis , Humans , Molecular Sequence Data , Nucleic Acid Hybridization , Phylogeny , Psychrobacter/genetics , Psychrobacter/physiology , RNA, Ribosomal, 16S/genetics , RNA, Ribosomal, 23S/genetics , Sequence Analysis, DNAABSTRACT
An analysis of 16S rRNA gene sequences from archived clinical reference specimens has identified two novel Neisseria species. For each species, two strains from independent sources were identified. Amongst species with validly published names, the closest species to the newly identified organisms were Neisseria canis, N. dentiae, N. zoodegmatis, N. animaloris and N. weaveri. DNA-DNA hybridization studies demonstrated that the newly identified isolates represent species that are distinct from these nearest neighbours. Analysis of partial 23S rRNA gene sequences for the newly identified strains and their nearest neighbours provided additional support for the species designation. Bayesian analysis of 16S rRNA gene sequences suggested that the newly identified isolates belong to distinct but related species of the genus Neisseria, and are members of a clade that includes N. dentiae, N. bacilliformis and N. canis. The predominant cellular fatty acids [16â:â0, summed feature 3 (16â:â1ω7c and/or iso-15â:â0 2-OH) and 18â:â1ω7c], as well as biochemical and morphological analyses further support the designation of Neisseria wadsworthii sp. nov. (type strain 9715(T) =DSM 22247(T) =CIP 109934(T)) and Neisseria shayeganii sp. nov. (type strain 871(T) =DSM 22246(T) =CIP 109933(T)).
