ABSTRACT
Splenectomy in rabbits causes a temporary reduction in interferon responding capacity of Poly I:C, followed by a period of hyperreactivity. At the same time a fraction of interferon with slightly different physiocochemical properties emerges. This could indicate that the spleen plays a relatively important role in the interferon response to Poly I:C. Splenectomy could be followed by the development of a compensatory cell population responsible for the production of interferon with slightly different molecular weight, isoelectric point and stability in urea.
Subject(s)
Interferons/blood , Poly I-C/pharmacology , Spleen/physiology , Animals , Drug Stability , Female , Interferons/analysis , Isoelectric Point , Male , Molecular Weight , Rabbits , Splenectomy , Urea/pharmacologySubject(s)
Interferons/therapeutic use , Adult , Animals , Antibody Formation , Chemical Phenomena , Chemistry, Physical , Child , Haplorhini , Humans , Interferon Inducers/therapeutic use , Interferons/biosynthesis , Interferons/physiology , Mice , Species Specificity , Viral Interference , Virulence , Virus Diseases/therapySubject(s)
Interferons/blood , Newcastle disease virus , Animals , Chromatography, Gel , Humans , Infant , Interferons/analysis , Leukocytes/metabolism , Lymphocytes/metabolism , Male , Molecular Weight , Rabbits , Spleen/cytology , Spleen/metabolism , Urea/pharmacology , Vesicular stomatitis Indiana virus , Viral Plaque AssaySubject(s)
Endotoxins/pharmacology , Leukocytes/metabolism , Newcastle disease virus , Pyrogens/biosynthesis , Animals , Ascitic Fluid/metabolism , Cell Survival , Cells, Cultured , Chromatography , Escherichia coli , Fever/chemically induced , Hemagglutination Tests , Kinetics , Leukocytes/analysis , Leukocytes/drug effects , Leukocytes/microbiology , Lymphocytes/drug effects , Lymphocytes/metabolism , Lymphocytes/microbiology , Pyrogens/analysis , Rabbits , Stimulation, ChemicalSubject(s)
Dactinomycin/pharmacology , Endotoxins/pharmacology , Leukocytes/metabolism , Newcastle disease virus , Orthomyxoviridae , Pyrogens/biosynthesis , Animals , Ascitic Fluid , Body Temperature , Depression, Chemical , Escherichia coli , Female , Immune Sera , In Vitro Techniques , Injections, Intraperitoneal , Interferons/biosynthesis , Interferons/blood , Male , Pyrogens/blood , RabbitsSubject(s)
Antiviral Agents/pharmacology , DNA Viruses/drug effects , Pyrimidines/pharmacology , RNA Viruses/drug effects , Animals , Deoxyuridine/pharmacology , Fluorouracil/pharmacology , Friend murine leukemia virus/drug effects , Kidney/drug effects , Mice , Pyrimidines/toxicity , Rabbits , Simplexvirus/drug effects , Spleen , Time Factors , Uracil/pharmacology , Vaccinia virus/drug effectsSubject(s)
Interferons/blood , Newcastle disease virus/pathogenicity , Orthomyxoviridae/pathogenicity , Animals , Chromatography , Dialysis , Female , Filtration , Gels , Guanidines/pharmacology , Hot Temperature , Injections, Intravenous , Interferons/isolation & purification , Male , Molecular Weight , Rabbits , Urea/pharmacologyABSTRACT
Experiments were performed to characterize the pyrogenic principle of ribonucleic acid (RNA) from the yeast Candida utilis. It was shown that ribonuclease hydrolysis of the RNA does not lead to inactivation of the pyrogenicity. Pyrogenicity was, however, destroyed by treatment with sodium deoxycholate. On column chromatography with Biogel under sterile and pyrogen-free conditions, the pyrogenic principle of yeast RNA was eluted together with the RNA. After treatment of the RNA with ribonuclease, it was possible to separate the pyrogenic activity from the RNA (hydrolysis products) to a great extent. Column chromatography of Escherichia coli endotoxin showed that the endotoxin was eluted in the same fractions as the pyrogenic activity of yeast RNA. On the basis of the behavior of the pyrogen, it may very well be that the fever reaction is produced not by the nucleic acid but by pyrogenic contaminants of the RNA preparation.
