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1.
J Microsc ; 268(3): 230-238, 2017 12.
Article in English | MEDLINE | ID: mdl-28686305

ABSTRACT

We have developed an analytical method to determine the segregation levels on the same tilt boundaries (TBs) at the same nanoscopic location by a joint use of atom probe tomography and scanning transmission electron microscopy, and discussed the mechanism of oxygen segregation at TBs in silicon ingots in terms of bond distortions around the TBs. The three-dimensional distribution of oxygen atoms was determined at the typical small- and large-angle TBs by atom probe tomography with a low impurity detection limit (0.01 at.% on a TB plane) simultaneously with high spatial resolution (about 0.4 nm). The three-dimensional distribution was correlated with the atomic stress around the TBs; the stress at large-angle TBs was estimated by ab initio calculations based on atomic resolution scanning transmission electron microscopy data and that at small-angle TBs were calculated with the elastic theory based on dark-field transmission electron microscopy data. Oxygen atoms would segregate at bond-centred sites under tensile stress above about 2 GPa, so as to attain a more stable bonding network by reducing the local stress. The number of oxygen atoms segregating in a unit TB area NGB (in atoms nm-2 ) was determined to be proportional to both the number of the atomic sites under tensile stress in a unit TB area nbc and the average concentration of oxygen atoms around the TB [Oi ] (in at.%) with NGB ∼ 50 nbc [Oi ].

2.
Vet J ; 214: 21-3, 2016 Aug.
Article in English | MEDLINE | ID: mdl-27387721

ABSTRACT

Reproductive management is necessary to prevent deleterious genetic disorders in purebred dogs, but comprehensive studies aimed at prevention of multiple underlying genetic disorders in a single breed have not been performed. The aims of this study were to examine mutant allele frequencies associated with multiple genetic disorders, using Border collies as a representative breed, and to make recommendations for prevention of the disorders. Genotyping of known mutations associated with seven recessive genetic disorders was performed using PCR assays. More than half (56%) of the Border collies had no mutant alleles associated with any of the seven disorders, suggesting that these disorders can be removed from the population over several generations. Since frequencies of each mutant allele differed among disorders, reproductive management should be performed after the establishment of prevention schemes that are appropriate for each disorder, the type and specificity of genetic test available, and the effective population size in each breeding colony.


Subject(s)
Dog Diseases/epidemiology , Gene Frequency , Genetic Diseases, Inborn/veterinary , Animals , Breeding , Dog Diseases/genetics , Dogs , Genetic Counseling , Genetic Diseases, Inborn/epidemiology , Genetic Diseases, Inborn/genetics , Genotype , Japan/epidemiology , Mutation , Polymerase Chain Reaction/veterinary , Prevalence
4.
J Chem Phys ; 139(14): 144705, 2013 Oct 14.
Article in English | MEDLINE | ID: mdl-24116639

ABSTRACT

The atomic and electronic structures of NiO(001)∕Au(001) interfaces were analyzed by high-resolution medium energy ion scattering (MEIS) and photoelectron spectroscopy using synchrotron-radiation-light. The MEIS analysis clearly showed that O atoms were located above Au atoms at the interface and the inter-planar distance of NiO(001)∕Au(001) was derived to be 2.30 ± 0.05 Å, which was consistent with the calculations based on the density functional theory (DFT). We measured the valence band spectra and found metallic features for the NiO thickness up to 3 monolayer (ML). Relevant to the metallic features, electron energy loss analysis revealed that the bandgap for NiO(001)∕Au(001) reduced with decreasing the NiO thickness from 10 down to 5 ML. We also observed Au 4f lines consisting of surface, bulk, and interface components and found a significant electronic charge transfer from Au(001) to NiO(001). The present DFT calculations demonstrated the presence of an image charge beneath Ni atoms at the interface just like alkali-halide∕metal interface, which may be a key issue to explain the core level shift and band structure.

5.
Eye (Lond) ; 20(7): 796-800, 2006 Jul.
Article in English | MEDLINE | ID: mdl-16021186

ABSTRACT

PURPOSE: To investigate control mechanisms for ocular blood flow changes after dynamic exercise using two different methods. METHODS: Changes over time in the tissue blood flow in the retina and choroid-retina of healthy volunteers were determined after dynamic exercise (Master's double two-step test), using scanning laser Doppler flowmetry (SLDF) and laser speckle flowgraphy (LSFG). Changes in intraocular pressure (IOP), blood pressure, plasma CO(2) gas concentration (pCO(2)), and levels of nitric oxide (NO) metabolites were examined. RESULTS: Retinal blood flow measured by SLDF increased significantly only at 15 min after exercise. In contrast, normalized blur (NB) values in the choroid-retina, obtained by LSFG, increased significantly up to 60 min after exercise. Ocular perfusion pressure (OPP), calculated from IOP and blood pressure, increased significantly immediately and 15 min after exercise. The plasma NO metabolite levels increased significantly, although pCO(2) levels were unchanged. CONCLUSIONS: Dynamic exercise changes OPP and produces increased tissue blood flow in the retina in the immediate postexercise period, while blood flow increases more persistently in the choroid-retina. Difference in control of blood flow in these two regions may be related to stronger autoregulatory mechanism of blood flow in the retina. Nitric oxide may play a role in the regulation of blood flow.


Subject(s)
Blood Flow Velocity/physiology , Choroid/blood supply , Exercise/physiology , Retina/physiology , Adult , Blood Gas Analysis , Blood Pressure/physiology , Carbon Dioxide/blood , Female , Humans , Intraocular Pressure/physiology , Laser-Doppler Flowmetry , Male , Middle Aged , Nitric Oxide/blood , Reference Values , Regional Blood Flow
6.
Eur J Immunol ; 31(12): 3659-66, 2001 Dec.
Article in English | MEDLINE | ID: mdl-11745386

ABSTRACT

Transforming growth factor-beta1 (TGF-beta1) is an inhibitory cytokine increasingly recognized as a key factor for immuno-regulation. The function of IL-4 in the regulation of TGF-beta1 production from T cells has been reported previously; however, the precise molecular mechanism still remains to be elucidated. For a better understanding of the mechanism involved in regulation, we have investigated a relationship between the STAT6-dependent pathway and TGF-beta1 production from naïve T cells. TCR crosslinking initiates TGF-beta1 production in CD4(+) T cells, and IL-4-mediated signaling enhances the TGF-beta1 production from naïve CD4(+) T cells. The IL-4-mediated up-regulation of TGF-beta1 production from naïve CD4(+) T cells is elicited in STAT6-deficient (STAT6 KO) mice, but not in IL-4 receptor-deficient (IL-4R KO) mice. These results clearly demonstrate that a STAT6-independent pathway is working in IL-4-mediated enhancement of TGF-beta1 production from naïve CD4(+) T cells. Moreover, the addition of IL-4 showed no additive effect on TGF-beta1 promoter-mediated transcription stimulated by TCR. Therefore, we hypothesize that the IL-4-mediated signaling does not work directly on the transcription of the TGF-beta1 gene, but rather regulates the expansion of TGF-beta1-secreting T cells.


Subject(s)
CD4-Positive T-Lymphocytes/metabolism , Interleukin-4/pharmacology , Trans-Activators/physiology , Transforming Growth Factor beta/biosynthesis , Animals , Female , Interferon-gamma/biosynthesis , Interleukin-10/biosynthesis , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Promoter Regions, Genetic , Receptors, Interleukin-4/physiology , STAT6 Transcription Factor , Transforming Growth Factor beta/genetics
7.
Cancer Lett ; 153(1-2): 63-6, 2000 May 29.
Article in English | MEDLINE | ID: mdl-10779631

ABSTRACT

Tumor formation was examined in mice in which the beta-chain gene of alphabetaT was knocked out (alphabetaT-KO mice) or the delta-chain gene of gammadeltaT was knocked out (gammadeltaT-KO mice). Development of Hepa 1-6 cell hepatoma was observed in six of six alphabetaT-KO mice, in four of six wild-type mice, and in only one of six gammadeltaT-KO mice. These results imply that gammadeltaT cells play a role in suppression of killer cell activity in tumor-bearing mice.


Subject(s)
Carcinoma, Hepatocellular/immunology , Receptors, Antigen, T-Cell, gamma-delta/physiology , T-Lymphocytes/physiology , Animals , Carcinoma, Hepatocellular/genetics , Cell Transformation, Neoplastic/genetics , Female , Male , Mice , Mice, Inbred C57BL , Mice, Knockout , Neoplasm Transplantation , Receptors, Antigen, T-Cell, gamma-delta/genetics , T-Lymphocytes/immunology , Tumor Cells, Cultured
8.
Proc Natl Acad Sci U S A ; 96(13): 7451-5, 1999 Jun 22.
Article in English | MEDLINE | ID: mdl-10377435

ABSTRACT

We analyzed the cytolytic activity of intraepithelial T cells (IEL) isolated from the small intestines of 2- to 3-month-old mutant mice rendered deficient in different gene(s) in which the number of IEL expressing either T cell receptor (TCR)-alpha beta (alpha beta-IEL) or TCR-gamma delta (gamma delta-IEL) were absent or markedly diminished. When compared with wild-type littermates, cytolytic activity of gamma delta-IEL was sharply attenuated in TCR-beta mutant mice but remained unaltered in TCR-alpha mutant mice in which a minor population of dull TCR-beta+ (betadim)-IEL was also present. Cytolytic activity of gamma delta-IEL was maintained in mice doubly homozygous for beta2-microglobulin and transporter associated with antigen processing 1 gene mutations in which a conspicuous decrease was noted in absolute numbers of alpha beta-IEL. In contrast, both TCR-delta and IL-7 receptor-alpha gene mutations that lead to lack of gamma delta-IEL generation did not affect the development or cytolytic activity of the remaining alpha beta-IEL. The anti-CD3 and anti-TCR-gamma delta mAb-induced IFN-gamma production of gamma delta-IEL showed the same TCR-alpha and TCR-beta mutation-dependent variability. These results indicate that cytolytic and IFN-gamma-producing activities of gamma delta T cells in mouse intestinal epithelium are TCR-beta-chain-dependent.


Subject(s)
Interferon-gamma/immunology , Intestinal Mucosa/immunology , Receptors, Antigen, T-Cell, alpha-beta/immunology , Receptors, Antigen, T-Cell, gamma-delta/immunology , T-Lymphocytes/immunology , Animals , Gene Expression Regulation/immunology , Immunity, Mucosal , Mice , Mutation , Receptors, Antigen, T-Cell, alpha-beta/genetics , Receptors, Antigen, T-Cell, gamma-delta/genetics
9.
Life Sci ; 64(14): 1243-52, 1999.
Article in English | MEDLINE | ID: mdl-10210267

ABSTRACT

In this study, we determined the activities of four aminopeptidases such as aminopeptidase B (APB), M (APM), N (APN) and dipeptidylpeptidase IV (DPP IV) in Caco-2 cells and compared with those in the rat intestinal mucosae. The activities of APB, APM and APN appeared to be highest in rat small intestinal mucosa, while DPP IV activity was much higher in Caco-2 cells than that in the rat intestinal mucosa. Next the inhibitory effects of various protease inhibitors were examined in Caco-2 homogenate. Three tested inhibitors, bacitracin, amastatin and puromycin, effectively inhibited the activities of APM, APN and DPP IV except for APB. Further, we quantitatively evaluated the permeation and degradation properties of leucine enkephalin (Leu-Enk) in the presence or absence of inhibitors in Caco-2 monolayer system. Leu-Enk had a high degradation clearance (CLd) and a low permeation clearance (CLp) in Caco-2 monolayers. This finding indicates that the very rapid degradation of Leu-Enk on the apical side of Caco-2 monolayers was due to aminopeptidases. However, these protease inhibitors besides sodium glycocholate were able to reduce the CLd values markedly, thereby increasing the permeation amount of Leu-Enk across Caco-2 monolayers. In particular, amastatin significantly decreased the CLd value and increased the CLp value. This enhanced CLp value was further increased by the coadministration with an absorption enhancer, EDTA or laurylmaltoside. These findings are relevant to the oral administration of peptide drugs and to developing an efficient oral delivery system.


Subject(s)
Enkephalin, Leucine/pharmacokinetics , Intestinal Absorption , Aminopeptidases/antagonists & inhibitors , Aminopeptidases/metabolism , Animals , Biological Transport/drug effects , Caco-2 Cells , Humans , Protease Inhibitors/pharmacology , Rats , Rats, Wistar
10.
Jpn J Cancer Res ; 89(10): 1041-6, 1998 Oct.
Article in English | MEDLINE | ID: mdl-9849583

ABSTRACT

Direct activation of human cytotoxic T lymphocytes (CTL) by interleukin (IL)-18 was observed in a system in which CTL effective against autologous tumor cells were generated. Peripheral blood mononuclear cells (PBMC) from tumor-bearing patients, after removal of natural killer (NK) cells, were cultured in a medium containing IL-1, -2, -4, and -6, with or without IL-18, and stimulated with autologous tumor cells. IL-18 increased the activity of the CTL and the proportion of autologous CD8+ T cells present after 28 days in the induction culture. When purified CD8+ T cells were cultured in the presence of IL-18 and IL-2 for 7 days, the CTL showed enhanced cytotoxic activity against autologous tumor cells. Moreover, a purified CD8+ T cell population, which did not exhibit any apparent cytotoxic activity against autologous tumor cells, displayed cytotoxic activity after 7-day incubation with IL-18. These results suggest that IL-18 may be useful to generate autologous CTL in humans and may thereby contribute to adoptive immunotherapy for tumors.


Subject(s)
Cytotoxicity, Immunologic , Interleukin-18/pharmacology , Interleukins/pharmacology , Lymphocyte Activation , Neoplasms/immunology , T-Lymphocytes, Cytotoxic/immunology , Adenocarcinoma , Carcinoma, Renal Cell , Cells, Cultured , Coculture Techniques , Glioblastoma , Humans , Interleukin-1/pharmacology , Interleukin-2/pharmacology , Interleukin-4/pharmacology , Interleukin-6/pharmacology , Kidney Neoplasms , Lymphocyte Activation/drug effects , Recombinant Proteins/pharmacology , Stomach Neoplasms , T-Lymphocytes, Cytotoxic/drug effects , Tumor Cells, Cultured
11.
Clin Immunol Immunopathol ; 88(3): 277-86, 1998 Sep.
Article in English | MEDLINE | ID: mdl-9743615

ABSTRACT

Modulation of CD8(+) T-cell responses specific for an exogenous antigen by epitope variants would be advantageous to develop a novel means of antigen-specific immune regulation. We have analyzed CD8(+) T-cell responses to single amino acid-substituted variants of a peptide corresponding to residues 142-149 (p142-149; LAYFYPEL) of alphas1-casein, a major milk allergen, which is a dominant determinant restricted by H-2Kb. An analog peptide L142I with a substitution of Ile for Leu at the nonanchor N-terminal residue induced more IFN-gamma secretion than p142-149 from specific CD8(+) T cells. Furthermore, L142I could prime CD8(+) T cells more efficiently in vivo, and these L142I-primed cells secreted more IFN-gamma than p142-149-primed CD8(+) T cells upon stimulation with p142-149 in vitro. These findings are mainly explained by the greater ability of L142I to form stable Kb-peptide complexes. These findings indicate that appropriate analog peptides may be useful as efficient inducers of CD8(+) T cells which recognize the parent peptide and secrete IFN-gamma, a potent inhibitor of Th2-dependent events, including IgE production.


Subject(s)
CD8-Positive T-Lymphocytes/drug effects , CD8-Positive T-Lymphocytes/metabolism , Caseins/immunology , Caseins/pharmacology , Epitopes/immunology , Interferon-gamma/biosynthesis , Oligopeptides/immunology , Oligopeptides/pharmacology , Peptide Fragments/immunology , Peptide Fragments/pharmacology , Amino Acid Sequence , Animals , Caseins/metabolism , Cattle , Epitopes/metabolism , Female , H-2 Antigens/immunology , H-2 Antigens/metabolism , Interferon-gamma/immunology , Interferon-gamma/metabolism , Lymphocyte Activation/drug effects , Mice , Mice, Inbred C57BL , Molecular Sequence Data , Oligopeptides/chemical synthesis , Peptide Fragments/metabolism , Sequence Homology, Amino Acid , Stimulation, Chemical
12.
FEBS Lett ; 423(2): 138-42, 1998 Feb 20.
Article in English | MEDLINE | ID: mdl-9512346

ABSTRACT

The CD8+ T cell clone 5F1 produces interleukin 10 (IL-10) and interferon gamma(IFN-gamma) in response to stimulation with a peptide corresponding to region 142-149 of bovine alpha(s1)-casein (p142-149). Ninety analog peptides derived from p142-149 with single amino acid substitutions of putative T cell receptor contact residues were prepared to examine whether production of IL-10 and IFN-gamma by 5F1 can be altered by stimulation with these peptides. We found that some peptides triggered only IL-10 production whereas others induced production of IFN-gamma alone or both of these cytokines. Peptides inducing IFN-gamma production triggered both cytotoxicity and a proliferative response, whereas peptides inducing production of IL-10 but not IFN-gamma triggered neither of these responses. Our results clearly demonstrate that the signaling pathway required for IL-10 production in CD8+ T cells differs from that required for IFN-gamma production. The distinct cellular signals for IL-10 production appear to be independent of those for cytotoxicity and the proliferative response of CD8+ T cells.


Subject(s)
CD8-Positive T-Lymphocytes/physiology , Interleukin-10/biosynthesis , Peptides/pharmacology , Signal Transduction/drug effects , Animals , CD8-Positive T-Lymphocytes/immunology , CD8-Positive T-Lymphocytes/metabolism , Caseins/immunology , Caseins/isolation & purification , Cattle , Female , Interferon-gamma/biosynthesis , Mice , Mice, Inbred C57BL , Peptides/immunology , Point Mutation , T-Lymphocyte Subsets
13.
Kyobu Geka ; 50(10): 862-5, 1997 Sep.
Article in Japanese | MEDLINE | ID: mdl-9301183

ABSTRACT

A 62-year-old man with complaints of severe chest pain came to our hospital. An emergency coronary angiography was performed and he was diagnosed as having acute myocardial infarction. Due to severe triple vessels disease he was referred to the department of Cardiovascular Surgery to undergo emergency coronary artery bypass grafting. In the coronary care unit, sudden hematoemesis due to hemorrhagic gastric ulcer occurred, however, just when he was going to be transferred to the operation room. Because the gastric bleeding was thought to be serious under extracorporeal circulation, which was indispensable for coronary artery bypass grafting, gastrotomy with suturing ulcer was performed prior to median sternotomy with use of intraaortic balloon pumping. Severe infection was not complicated. His postoperative course was uneventful.


Subject(s)
Coronary Artery Bypass , Myocardial Infarction/surgery , Peptic Ulcer Hemorrhage/surgery , Stomach Ulcer/surgery , Stomach/surgery , Suture Techniques , Emergencies , Humans , Male , Middle Aged , Stomach Ulcer/complications
14.
Nihon Kyobu Geka Gakkai Zasshi ; 45(8): 1111-5, 1997 Aug.
Article in Japanese | MEDLINE | ID: mdl-9301239

ABSTRACT

A 58-year-old man, who had been submitted to an indication of mitral valvuloplasty (MVP) because of severe mitral regurgitation, was diagnosed as idiopathic thrombocytopenic purpura (ITP). In order to diminish the peri-operative blood loss, splenectomy and high-dose bolus administration of gamma-globulin (400 mg/kg/day) were performed at the time of two weeks and during last five days respectively, prior to MVP surgery. In early post-operative stage, anticoagulant therapy was held down considering ITP, and we were not troubled with bleeding throughout the peri-operative period. On the fourteenth post-operative day, however, mural thrombi in the left atrium were pointed out by transesophageal echocardiography (TEE). Then anticoagulant therapy was reinforced by increasing the warfarin dose. When TEE was restudied on 36th, post-operative day, mural thrombi had almost disappeared. Although it is commonly known that mitral valvuloplasty has low risk of thrombosis and less necessity of anticoagulation, hyper coagulability may be feasible in the early post-operative period of our case whose ITP is meticulously treated prior to surgery. Suitable anticoagulation should have been performed regardless of ITP immediately after MVP.


Subject(s)
Heart Diseases/etiology , Mitral Valve Insufficiency/surgery , Mitral Valve/surgery , Postoperative Complications , Purpura, Thrombocytopenic, Idiopathic/complications , Thrombosis/etiology , Heart Atria , Humans , Male , Middle Aged
15.
Nihon Kyobu Geka Gakkai Zasshi ; 45(8): 1208-12, 1997 Aug.
Article in Japanese | MEDLINE | ID: mdl-9301257

ABSTRACT

Elephant trunk prosthesis was devised for treatment of multiple aortic aneurysm. But it has applied to type A aortic dissection. Because residual false lumen often dilates gradually and come to have the risk of rupture. Two patients who were diagnosed as type A aortic dissection were performed total aortic arch replacement with Elephant trunk prosthesis on distal anastmosis. Postoperative diagnostic images showed that residual false lumen of proximal descending aorta were thrombosed. Now it is not necessary to perform next extensive aortic replacement. Complication which is threatened in using Elephant trunk prosthesis, paraplegia, thromboembolism, kinking of prosthesis could have been avoided by setting suitable length and diameter of the prosthesis.


Subject(s)
Aortic Aneurysm, Thoracic/surgery , Aortic Dissection/surgery , Blood Vessel Prosthesis Implantation , Aorta, Thoracic/surgery , Humans
16.
Biosci Biotechnol Biochem ; 61(7): 1156-62, 1997 Jul.
Article in English | MEDLINE | ID: mdl-9255980

ABSTRACT

alpha s1-Casein-specific CD8+ T cell clones expressed the interleukin (IL)-4 receptor, although they did not secrete detectable IL-4. We found that IL-4 significantly enhanced the secretion of interferon (IFN)-gamma by these CD8+ T cell clones. IL-4 also enhanced the secretion of IFN-gamma induced by stimulating the immobilized anti-CD3 antibodies of polyclonal CD8+ T cells which had been isolated from lymph nodes and were stimulated in vitro with the immobilized anti-CD3 antibody and IL-2. In addition, IL-4 added at the time of this first in vitro stimulation induced strong IFN-gamma productivity, as well as IL-4 and IL-10 productivity, which were detectable upon restimulation of these cells. Results are discussed in relation to the inhibitory effects of IFN-gamma production on IL-4-producing cells.


Subject(s)
CD8-Positive T-Lymphocytes/drug effects , CD8-Positive T-Lymphocytes/metabolism , Caseins/metabolism , Interferon-gamma/metabolism , Interleukin-4/pharmacology , Animals , Antigens, CD/drug effects , Antigens, CD/metabolism , CD3 Complex/pharmacology , CD8-Positive T-Lymphocytes/immunology , Caseins/pharmacology , Clone Cells , Female , Interferon-gamma/drug effects , Interleukin-2/pharmacology , Lymph Nodes/cytology , Mice , Mice, Inbred C57BL , Receptors, Interleukin/drug effects , Receptors, Interleukin/metabolism , Receptors, Interleukin-4
17.
Microbiol Immunol ; 41(4): 353-9, 1997.
Article in English | MEDLINE | ID: mdl-9159410

ABSTRACT

Intestinal intraepithelial T cells (IELs) expressing either gammadelta TCR or alphabeta TCR have been proposed to play an important role in the regulation of intestinal epithelia by producing cytokines that directly influence the adjoining intestinal epithelial cell (IEC) functions. To illuminate this issue, we utilized TCR mutant mice to obtain gammadelta IELs, alphabeta IELs and mixed gammadelta and alphabeta IELs from corresponding alphabeta T-cell-deficient (beta-/-), gammadelta T-cell-deficient (delta-/-) and wild-type (WT) littermate mice. The production of IFN-gamma by these IELs as well as the mRNA for IFN-gamma, TGF-alpha, TGF-beta1, TNF-alpha and TNF-beta in these IELs, in conjunction with the effect of produced cytokines on the expression of class II MHC molecules by the in vitro cell line IEC-6, were investigated. IFN-gamma and TNF-alpha [corrected] specific mRNA were detectable in all freshly isolated gammadelta, alphabeta and WT IELs. In addition to the IFN-gamma and TNF-alpha [corrected] mRNA, alphabeta and WT IELs that had been activated in culture plates coated with anti-CD3 mAb contained mRNA for TGF-beta1 and TNF-beta proteins. In the cultured gammadelta IELs, however, the signals for IFN-gamma and TNF-alpha [corrected] transcripts were weak, and mRNA for the latter two cytokines was almost undetectable. Supernatants from in vitro culturing of alphabeta and WT IELs but not gammadelta IELs induced class II MHC gene expression in IEC-6, whereas, in the presence of anti-IFN-gamma mAb, the same culture supernatants failed to do so. In fact, the concentration of IFN-gamma in supernatants from alphabeta and WT IEL cultures was ten- to twentyfold higher than that in the supernatant from the gammadelta IEL culture. Finally, TGF-alpha specific mRNA was not detectable in the gammadelta and alphabeta IELs even after in vitro activation. These results indicate that alphabeta IELs are superior to gammadelta IELs in the ability to produce IFN-gamma, TGF-beta1, TNF-alpha [corrected] and TNF-beta through TCR crosslinking primary in vitro stimulation.


Subject(s)
Cytokines/metabolism , Intestines/immunology , Receptors, Antigen, T-Cell, alpha-beta/immunology , Receptors, Antigen, T-Cell, gamma-delta/immunology , T-Lymphocytes/immunology , Animals , CD3 Complex/immunology , Cells, Cultured , Epithelium/immunology , Histocompatibility Antigens Class II/metabolism , Interferon-gamma/metabolism , Lymphotoxin-alpha/metabolism , Mice , Mice, Inbred C57BL , Mice, Mutant Strains , RNA, Messenger/metabolism , Receptors, Antigen, T-Cell, alpha-beta/genetics , Receptors, Antigen, T-Cell, gamma-delta/genetics , Transforming Growth Factor alpha/metabolism , Transforming Growth Factor beta/metabolism , Tumor Necrosis Factor-alpha/metabolism
18.
Cytotechnology ; 25(1-3): 89-100, 1997 Nov.
Article in English | MEDLINE | ID: mdl-22358883

ABSTRACT

Feeding of a whole casein diet, which abolished the α(s1)-casein-specific proliferation and IFN-γ productivity of CD(4+) T cells, did not affect the proliferative response of CD8(+) T cells with regard to the antigen dose response, cell dose response, kinetics of the proliferation and epitope specificity, as well as IFN-γ production. To assess the characteristics of the CD8(+) T cells, we established α(s1)-casein-specific CD8(+) T cell clones from both casein-fed and control mice. The established clones produced different amount of IFN-γ and IL-10, and one clone derived from the casein-fed mice produced a remarkable amount of IL-10. The clones from casein-fed mice produced considerable amounts of TGF-ß, while those from control mice produced only small amounts. The possible role of CD8(+) T cells in oral tolerance is discussed.

19.
Cell Immunol ; 172(2): 200-4, 1996 Sep 15.
Article in English | MEDLINE | ID: mdl-8964081

ABSTRACT

CD8+ T cell clones produced both interleukin 10 (IL-10) and interferon-gamma (IFN-gamma) when these cells were stimulated with a T-cell-specific mitogen, concanavalin A (Con A). One of these CD8+ T cell clones, 13G2, secreted IFN-gamma at similar levels with calcium ionophore, A23187, as well as by Con A, but IL-10 production by A23187 was less than by Con A. On the other hand, N6,O2-dibutyryl cAMP enhanced the production of IL-10 but not IFN-gamma when the low doses of Con A or A23187 coexisted. In a T cell clone, the production of these two cytokines required different signal transductions. These results indicate that a T cell clone can produce diverse cytokines depending on the surrounding condition.


Subject(s)
CD8-Positive T-Lymphocytes/metabolism , Interferon-gamma/biosynthesis , Interleukin-10/biosynthesis , Signal Transduction/immunology , Animals , CD8-Positive T-Lymphocytes/enzymology , CD8-Positive T-Lymphocytes/immunology , Calcium/metabolism , Clone Cells/enzymology , Clone Cells/immunology , Clone Cells/metabolism , Concanavalin A/pharmacology , Cyclic AMP/metabolism , Cyclic AMP/physiology , Enzyme Activation/drug effects , Enzyme Activation/immunology , Female , Interferon-gamma/drug effects , Interferon-gamma/physiology , Interleukin-10/physiology , Lymphocyte Activation , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Mice, Nude , Protein Kinase C/metabolism , Receptors, Antigen, T-Cell/drug effects , Receptors, Antigen, T-Cell/immunology , Signal Transduction/drug effects
20.
Phys Rev B Condens Matter ; 51(19): 13111-13116, 1995 May 15.
Article in English | MEDLINE | ID: mdl-9978109
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