ABSTRACT
The effect of ultrasound (frequency 0.88 MHz, intensity from 0.05 to 1 W/cm2) on alterations in antigenic activity has been investigated in vitro using ABO antigens of human erythrocytes. The existence of threshold doses of ultrasound influence has been found. These doses are shown to be independent of ultrasound intensity. The dependence of the effect on erythrocyte concentration has been established. Individual and group differences in the antigenic resistance to ultrasonic exposure in donors of groups A and B have been revealed. A drop in antigenic activity equal to 97% has been obtained.
Subject(s)
ABO Blood-Group System/immunology , Epitopes/analysis , Erythrocyte Membrane/immunology , Sonication , Ultrasonics , Humans , In Vitro TechniquesABSTRACT
A technique of antierythrocyte antibody detection is described. It as based on co-agglutination of red blood cells and S. aureus cells. The method is unsophisticated and non-laborious. It provides massive agglutination, its sensitivity is one order of magnitude higher than that of the Coombs test.
Subject(s)
Autoantibodies/analysis , Erythrocytes/immunology , Hemagglutination Tests/methods , Anemia, Hemolytic, Autoimmune/immunology , Humans , Staphylococcus aureus/immunologySubject(s)
Anemia, Hemolytic, Autoimmune/diagnosis , Child, Preschool , Female , Humans , Infant , Infant, Newborn , MaleSubject(s)
Anemia, Hemolytic, Congenital/blood , Erythrocyte Membrane/immunology , Erythrocytes/immunology , Membrane Proteins/immunology , Adult , Blood Protein Electrophoresis , Electrophoresis, Polyacrylamide Gel , Hemagglutination Tests/methods , Humans , Immunoglobulin M/isolation & purification , Male , Membrane Proteins/analysisABSTRACT
A new test for detection of antierythrocyte antibodies is described; one of its variants is three orders of magnitude more sensitive than the classic Coombs test. The proposed procedures are based on the agglutination of the red blood cells following addition of test-erythrocytes coated with the aggregated proteins of an antiglobulin serum. Results of application of the technique for diagnosis of the autoimmune hemolytic anemia showed its high specificity and the absence of prozone phenomenon.