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1.
Spine Surg Relat Res ; 7(5): 436-442, 2023 Sep 27.
Article in English | MEDLINE | ID: mdl-37841035

ABSTRACT

Introduction: Imaging analysis of foraminal stenosis in the fifth lumbar (L5) nerve root remains to be a challenge because of the anatomical complexity of the lumbosacral transition. T2-weighted three-dimensional (3D) magnetic resonance images (MRI) have been dominantly used for diagnosis of lumbar foraminal stenosis, while the reliability of T1-weighted images (WI) has also been proven. In this study, we aim to compare the reliability and reproducibility of T1- and T2-weighted 3D MRI in diagnosing lumbar foraminal stenosis (LFS) of the L5 nerve root. Methods: In this study, 39 patients with unilateral L5 radiculopathy (20 had L4-L5 intracanal stenosis; 19 had L5-S foraminal stenosis) were enrolled, prospectively. T1- and T2-weighted 3D lumbar MRI were obtained from each patient. T1WI and T2WI were blinded and then separately reviewed twice by four examiners randomly. The examiners were instructed to answer the side of LFS or absence of LFS. The correct answer rate, sensitivity, specificity, and area under the curve were analyzed and compared between T1WI and T2WI. Also, intra- and interobserver agreements were calculated using kappa (κ)-statistics and compared in the same manner. Results: The average correct answer rate, sensitivity, specificity, and area under the curve of the T1WI/T2WI were 84.6%/80.1%, 82.9%/80.3%, 86.3%/81.3%, and 0.846/0.801, respectively. The intraobserver κ-values of the four examiners ranged from 0.692 to 0.916 (average: 0.762) and from 0.669 to 0.801 (average: 0.720) for T1WI and T2WI, respectively. The interobserver κ-values calculated in a round-robin manner (24 combinations in total) ranged from 0.544 to 0.790 (average: 0.657) and from 0.524 to 0.828 (average: 0.652), respectively. Conclusions: As per our findings, T1- and T2-weighted 3D MRI were determined to have nearly equivalent reliability and reproducibility in terms of diagnosing LFS of the L5 nerve root.

3.
Clin Spine Surg ; 33(10): E478-E485, 2020 12.
Article in English | MEDLINE | ID: mdl-32282403

ABSTRACT

STUDY DESIGN: A prospective multicenter study. OBJECTIVE: The purpose of this study was to determine whether laminoplasty (LP) is comparable for myelopathy caused by cervical disk herniation (CDH). SUMMARY OF BACKGROUND DATA: Anterior decompression and fusion (ADF) has conventionally been used for myelopathy caused by CDH with stable outcomes. However, recurrence of myelopathy due to adjacent segment degeneration are its drawbacks. The efficacy of LP without discectomy has been sporadically reported, but no long-term prospective study has been conducted to verify it. MATERIALS AND METHODS: Patients with cervical myelopathy caused by CDH were studied. The first 30 patients and the next 30 patients were treated with ADF and LP, respectively. The outcomes were compared between the 22 ADF patients and the 20 LP patients who had completed the follow-up examination scheduled 10 years after surgery. RESULTS: There was no statistically significant difference in the postoperative severity or recovery rate of myelopathy between the 2 groups 10 years after surgery. One patient in the ADF group underwent LP for secondary myelopathy due to adjacent segment degeneration 2 years after the surgery. Reoperation was not required for patients in the LP group. Postoperative neck pain was significantly more severe in the LP group than in the ADF group. CONCLUSIONS: ADF and LP for cervical myelopathy caused by CDH achieve similarly favorable outcomes. Recurrence of myelopathy caused by adjacent segment degeneration is a disadvantage of ADF while residual neck pain is a disadvantage of LP.


Subject(s)
Laminoplasty , Spinal Cord Diseases , Spinal Fusion , Cervical Vertebrae/surgery , Decompression, Surgical , Humans , Prospective Studies , Spinal Cord Diseases/etiology , Spinal Cord Diseases/surgery , Treatment Outcome
4.
J Neurosurg Sci ; 64(6): 525-530, 2020 Dec.
Article in English | MEDLINE | ID: mdl-29308631

ABSTRACT

BACKGROUND: Japan has had a rapidly aging population during the past 30 years. This study aimed to investigate longitudinal changes in the surgical rate for spinal disorders in Miyagi Prefecture (2.35 million inhabitants) with a similar population composition to Japan. METHODS: Data of spinal surgeries were collected using the spine registry by Tohoku University Spine Society. Data on the annual number of spinal surgeries between 1988 and 2014 of all populations, in those aged ≥65 years old, in those aged ≥75 years old, and for each pathology were collected. The annual surgical rate per 100,000 inhabitants was calculated. RESULTS: The surgical rate in 2010-2014 in total, at ≥65 years old, and at ≥75 years old showed 3.2-, 3.8- and 7.1-fold increases, respectively, compared with that in 1988-1989. Degenerative spinal disorders, spinal trauma and pyogenic spondylitis markedly increased, while metastatic spinal tumor and tuberculous spondylitis decreased over time. The surgical rate at ≥75 years with lumbar spinal stenosis showed a 12.6-time increase. CONCLUSIONS: During a rapid period of aging, the rate of spinal surgeries has markedly increased, particularly, that for degenerative disorders. This is the first report on the long-term longitudinal changes in the rate of spinal surgery.


Subject(s)
Spinal Stenosis , Aged , Aging , Humans , Japan , Lumbar Vertebrae , Neurosurgical Procedures , Spinal Stenosis/surgery
5.
Spine Surg Relat Res ; 3(4): 304-311, 2019.
Article in English | MEDLINE | ID: mdl-31768449

ABSTRACT

INTRODUCTION: Laminoplasty is a common surgery for cervical myelopathy. Previous studies have analyzed the reoperation rates in posterior decompression surgeries of the cervical spine. However, few studies have solely focused on midline-splitting laminoplasty (MSL) using a large number of patients. This aims to analyze the reoperation rates after MSL using the survival function method. METHODS: Between 1988 and 2013, 4,208 MSLs were performed as a primary operation for cervical myelopathy and enrolled in our spinal surgery registration system. The Kaplan-Meier survival function method was used to analyze the rates of reoperation. RESULTS: Of 4,208 patients with primary MSL, 40 underwent reoperation for neurological complications. The overall reoperation rate was 0.26%, 0.64%, 0.83%, 0.93%, and 0.95% at 1, 5, 10, 20, and >20 years, respectively. The causes of reoperation were postoperative cervical radiculopathy in 10 patients, stenosis at an adjacent level in 8, stenosis due to failed "open-door" lamina in 6, instability of the cervical spine in 4, cervical disc herniation in 3, elongation of ossification of the posterior longitudinal ligament in 3, spinal cord injury in 1, fracture of the cervical spine in 1, postoperative scar formation in 1, ossification of anterior longitudinal ligament in 1, and unknown in 2. The number of patients with surgical site infection (SSI) who needed surgical debridement was 34 (0.81%). CONCLUSIONS: Excluding reoperations for SSI, the reoperation rate of MSL was approximately 1.0% at the maximum of 26 years after surgery. MSL was determined to be a reliable surgical procedure regarding postoperative complications requiring additional surgeries.

6.
J Orthop Sci ; 21(5): 579-85, 2016 Sep.
Article in English | MEDLINE | ID: mdl-27527658

ABSTRACT

BACKGROUND: There are no diagnostic support tools composed of a simple, single-sheet, self-administered, self-reported history questionnaire (SSHQ) for patients with leg symptoms caused by either lumbar spinal stenosis (LSS) or lumbar disc herniation (LDH), at the same time, can discriminate the two diseases. METHODS: We conducted retrospective and prospective derivation studies and a prospective validation study. Based on data from 137 patients with LSS and 206 with LDH, we identified key prediction factors to establish the diagnosis of LSS and LDH, which became the basis of a temporary SSHQ. Next, we performed a prospective derivation study in which 296 patients with LSS or LDH completed preoperatively this temporary SSHQ. After univariate and multivariate analyses of each question, questions on both diseases in addition to age factor were selected, providing the final version of the SSHQ. A validation study was subsequently performed with 342 consecutive patients with leg symptoms. The sensitivity, specificity and likelihood ratio of this SSHQ were calculated to determine the cut-off points for LSS and LDH. RESULTS: A SSHQ with 15 questions was developed from retrospective and prospective derivation studies. The score of each question was weighted based on the multivariate analysis and then, it was approximated to integer value. According to assessment of the discriminatory performance of the clinical prediction rule of the SSHQ, the cut-off point for LSS was ≥13 and that for LDH was ≥11. The sensitivity, specificity, and positive and negative likelihood ratios of this SSHQ at those cut-off points were, respectively, 92.7%, 84.7%, 6.07, and 0.09 for LSS, and 91.0%, 85.2%, 6.15, and 0.11 for LDH. CONCLUSIONS: This is the first report of a diagnostic support tool for patients with LSS- or LDH-induced leg symptoms combined in a single SSHQ that could help establish diagnosis of the two diseases in the daily clinical practice.


Subject(s)
Intervertebral Disc Displacement/complications , Neurologic Examination/methods , Peripheral Nervous System Diseases/diagnosis , Spinal Stenosis/complications , Surveys and Questionnaires , Aged , Cohort Studies , Female , Humans , Intervertebral Disc Displacement/diagnostic imaging , Japan , Lumbar Vertebrae/diagnostic imaging , Male , Middle Aged , Multivariate Analysis , Pain Measurement , Peripheral Nervous System Diseases/etiology , Physical Examination/methods , Predictive Value of Tests , Prospective Studies , ROC Curve , Reproducibility of Results , Retrospective Studies , Self Report , Severity of Illness Index , Spinal Stenosis/diagnostic imaging , Tomography, X-Ray Computed/methods
8.
Tohoku J Exp Med ; 238(2): 153-63, 2016 02.
Article in English | MEDLINE | ID: mdl-26876801

ABSTRACT

Spinal disorders affect mainly older people and cause pain, paralysis and/or deformities of the trunk and/or extremities, which could eventually disturb locomotive functions. For ensuring safe and high-quality treatment of spinal disorders, in 1987, the Tohoku University Spine Society (TUSS) was established by orthopedic departments in Tohoku University School of Medicine and its affiliated hospitals in and around Miyagi Prefecture. All spine surgeries have been enrolled in the TUSS Spine Registry since 1988. Using the data from this registration system between 1988 and 2012, we demonstrate here the longitudinal changes in surgical trends for spinal disorders in Japan that has rushed into the most advanced "aging society" in the world. In total, data on 56,744 surgeries were retrieved. The number of spinal surgeries has annually increased approximately 4-fold. There was a particular increase among patients aged ≥ 70 years and those aged ≥ 80 years, with a 20- to 90-fold increase. Nearly 90% of the spinal operations were performed for degenerative disorders, with their number increasing approximately 5-fold from 705 to 3,448. The most common disease for surgery was lumbar spinal stenosis (LSS) (35.9%), followed by lumbar disc herniation (27.7%) and cervical myelopathy (19.8%). In 2012, approximately half of the patients with LSS and cervical myelopathy were ≥ 70 years of age. In conclusion, the number of spinal operations markedly increased during the 25-year period, particularly among older patients. As Japan has a notably aged population, the present study could provide a near-future model for countries with aging population.


Subject(s)
Registries , Societies, Medical , Spinal Diseases/epidemiology , Spinal Diseases/surgery , Universities , Adult , Age Distribution , Aged , Aged, 80 and over , Female , Humans , Incidence , Japan/epidemiology , Male , Middle Aged , Young Adult
9.
Eur Spine J ; 24(2): 381-7, 2015 Feb.
Article in English | MEDLINE | ID: mdl-25073940

ABSTRACT

PURPOSE: Fenestration is the gold standard surgery for lumbar spinal canal stenosis in Japan. Several previous studies have analyzed the reoperation rates in large numbers of patients undergoing several surgical procedures such as laminectomy with or without instrumented spinal fusion; however, there have been few studies focusing solely on fenestration. The purpose of this study was to calculate the reoperation rates after fenestration using the survival function method. METHODS: Form 1988-2007, 6,998 surgeries for lumbar spinal canal stenosis occurred in Miyagi prefecture, Japan, and these patients were enrolled by the spinal surgery registration system of the Department of Orthopaedic Surgery, Tohoku University. Among these, 5,835 surgeries involved fenestration as a primary surgery and for those who underwent ≥2 lumbar surgeries we analyzed the reoperation rates using the Kaplan-Meier method. RESULTS: Among the 5,835 patients undergoing primary fenestration, 215 patients underwent 221 revisions; 112 included the same spinal levels and 103 were revised only at other levels as primary fenestration. The overall reoperation rates were 0.8% at 1 year, 2.9% at 5 years, 5.2% at 10 years, 7.5% at 15 years and 8.6% at >17.7 years. Reoperation rates for those at the same spinal levels were 0.6% at 1 year, 1.7% at 5 years, 2.7% at 10 years, 3.8% at 15 years, and 4.1% at >17.0 years. CONCLUSIONS: Fenestration can be performed at low cost using standard spinal surgery equipments. The reoperation rates of this procedure were lower than previously reported for several other surgical procedures.


Subject(s)
Decompression, Surgical/methods , Neurosurgical Procedures , Spinal Stenosis/surgery , Adult , Aged , Aged, 80 and over , Female , Follow-Up Studies , Humans , Lumbar Vertebrae/surgery , Male , Middle Aged , Neurosurgical Procedures/adverse effects , Neurosurgical Procedures/methods , Reoperation , Spinal Fusion/methods , Survival Analysis , Young Adult
10.
Clin Case Rep ; 3(12): 1021-5, 2015 12.
Article in English | MEDLINE | ID: mdl-26734139

ABSTRACT

The authors report a rare case of elderly-onset "lumbar spondylotic myelopathy" occurred on a low-placed spinal cord compressed at multiple levels with thickened ligamenta flava. A posterior decompression surgery could alleviate neurological symptoms successfully instead of untethering of the spinal cord, a widely accepted surgery for tethered cord.

12.
Eur Spine J ; 21(2): 282-8, 2012 Feb.
Article in English | MEDLINE | ID: mdl-21830078

ABSTRACT

PURPOSE: The combination of a facet fracture and a contralateral facet dislocation at the same intervertebral level of the cervical spine (a fracture and contralateral dislocation of the twin facet joints) has not been described in detail. The aims of this study are to report a series of 11 patients with this injury, to clarify the clinical features and to discuss its pathomechanism. METHODS: Among 251 patients with lower cervical spine fractures and/or dislocations surgically treated, 11 (9 males and 2 females, averaged age, 52 years) had this kind of injury. Medical charts and medical images were reviewed retrospectively. RESULTS: Injury levels were C4-5, C5-6 and C6-7 in 1, 4 and 6 patients, respectively. A fracture was found at the superior facet in 6, and at the inferior facet in 5. The anterior displacement of the vertebral body ranged from 7 to 19 mm. The unilateral horizontal facet appearance on an anteroposterior radiograph and the triple image on a CT composed of a separated fracture fragment, the base of the fractured facet, and the neighboring non-fractured facet were characteristic. All patients had neurological deficits from Frankel A to D, and were surgically treated by posterior fusion using wire or cable, or combined anterior and posterior spinal fusion. CONCLUSIONS: The fracture and contralateral dislocation of the twin facet joints can cause severe neurological deficits because of its gross anterior displacement. Its plausible pathomechanism is extension force exerted to the cervical spine when it is maximally bent laterally.


Subject(s)
Cervical Vertebrae/injuries , Spinal Fractures/complications , Zygapophyseal Joint/injuries , Accidents , Adult , Aged , Female , Humans , Male , Middle Aged , Retrospective Studies
13.
J Neurosurg Spine ; 15(1): 21-7, 2011 Jul.
Article in English | MEDLINE | ID: mdl-21495816

ABSTRACT

OBJECTIVE: Tethered cord syndrome (TCS) is a disorder involving an abnormal stretching of the tethered spinal cord caused by several pathological conditions and presents with a variety of neurological symptoms. Untethering (tethered cord release) is the gold standard treatment for TCS. However, untethering carries risks of spinal cord injury and postoperative retethering. To avoid these potential risks, the authors applied spine-shortening osteotomy to adult patients with TCS, and report on the surgical procedure and treatment outcomes. METHODS: Eight patients with TCS caused by a lipomyelomeningocele were surgically treated by the authors' original procedure of spine-shortening osteotomy. Six patients were male and 2 were females; average age at the time of surgery was 31 years old. Spine-shortening osteotomy was performed at the level of L-1 in all but 2 patients, in whom it was performed at T-12, with spinal fusion between T-12 and L-2 or T-11 and L-1 using a pedicle screw-rod system. The average follow-up period was 6.2 years and the patients' pre- and postoperative conditions were evaluated clinically and radiologically. RESULTS: Preoperatively, all patients displayed severe neurological deficits such as motor disturbance, muscle atrophy, and bladder dysfunction. Several months before surgery, all showed progressive symptoms. Those symptoms showed initial improvement in 6 patients and stabilized in 2 postoperatively, but the improved symptoms worsened again in 4 of the 6 patients. The osteotomized vertebrae were shortened by 21 mm on average, and all spines showed complete bone union without loss of correction. At the final follow-up evaluations, 6 patients showed stabilization as per the modified Japanese Orthopaedic Association score for thoracic myelopathy. CONCLUSIONS: Spine-shortening osteotomy successfully helps reduce the spinal cord tension without causing direct neural damage. At minimum, it stabilized the patients' symptoms and/or helped delay neurological deterioration for a period of time. Spine-shortening osteotomy might be a feasible mode of treatment for adult TCS caused by a spinal lipoma.


Subject(s)
Meningomyelocele/surgery , Neural Tube Defects/surgery , Osteotomy/methods , Adolescent , Adult , Bone Screws , Female , Humans , Lumbar Vertebrae/surgery , Male , Meningomyelocele/complications , Middle Aged , Neural Tube Defects/etiology , Recurrence , Spinal Fusion , Thoracic Vertebrae/surgery , Treatment Outcome
14.
Spine (Phila Pa 1976) ; 36(23): E1515-8, 2011 Nov 01.
Article in English | MEDLINE | ID: mdl-21252825

ABSTRACT

STUDY DESIGN: A retrospective case study. OBJECTIVE: To confirm the remodeling potential of the kyphotic malunited odontoid fracture in the pediatric spine. SUMMARY OF BACKGROUND DATA: Nonsurgical reduction and immobilization is the gold standard for the odontoid fracture in infancy. However, the reduction occasionally results in incomplete repositioning of the odontoid process. The cervical spine is subsequently immobilized until fusion in most cases in the hope of achieving remodeling with the growth of the remaining displacement and kyphotic angulation, although there are no precise data on the acceptable limit of the deformity. METHODS: Three patients (age at injury = 1 year 2 months to 3 years) with odontoid process fracture in infancy were treated conservatively and the fractures were observed on plain lateral radiographs until at least the age of 20 years. For evaluation of the angulated odontoid process, we used our original measurement method of the odontoid process tilting angle (OPTA). In addition, the OPTAs were also measured in 127 Japanese adult patients (57 male patients and 70 female patients; average age = 43 years) without a history of odontoid fracture, as normal controls. RESULTS: The OPTA in the normal controls was -21.4° ± 23.3°. The OPTAs ranged from 41° to 62° at the initial evaluation, from 12° to 30° at the time of bony union, and from -4° to -14° at the final follow-up at more than 20 years of age, which were all within one standard deviation of the mean in the normal adult controls. CONCLUSION: Angulated odontoid fractures with the OPTA around 30° at the time of bony union in infants younger than 3 years of age could have the capacity for remodeling to the normal morphology. No surgical reduction might be needed to recommend complete apposition of the odontoid process in the absence of severe or deteriorating neural impairment.


Subject(s)
Cervical Vertebrae/diagnostic imaging , Kyphosis/diagnostic imaging , Odontoid Process/diagnostic imaging , Spinal Fractures/diagnostic imaging , Adolescent , Adult , Bone Remodeling , Cervical Vertebrae/injuries , Cervical Vertebrae/surgery , Child , Child, Preschool , Female , Follow-Up Studies , Humans , Infant , Kyphosis/physiopathology , Kyphosis/surgery , Male , Odontoid Process/injuries , Odontoid Process/surgery , Orthopedic Procedures/methods , Radiography , Retrospective Studies , Spinal Fractures/physiopathology , Spinal Fractures/surgery , Time Factors , Treatment Outcome , Young Adult
15.
J Orthop Sci ; 15(1): 71-8, 2010 Jan.
Article in English | MEDLINE | ID: mdl-20151254

ABSTRACT

BACKGROUND: Anterior decompression and fusion (ADF) has conventionally been used, with stable outcomes, for cervical myelopathy caused by soft disc herniation. However, complications related to bone grafting and recurrence of myelopathy due to adjacent segment degeneration are its drawbacks. The efficacy of laminoplasty as an alternative has been sporadically reported, but no prospective study has been conducted to verify it. The purpose of this study was to determine whether laminoplasty is comparable for this condition. METHODS: Patients with cervical myelopathy caused by soft disc herniation whose preoperative disease period was less than 1 year were studied. The first 30 patients and the next 30 patients were treated by ADF and laminoplasty, respectively. All patients were given the same postoperative management. The outcomes were compared between the ADF and the laminoplasty groups consisting of 25 patients each who completed a follow-up examination 1 year after surgery. RESULTS: The two groups were found statistically matched regarding age at surgery, sex, disc level of herniation, anteroposterior diameter of the spinal canal, preoperative severity of myelopathy, cervical lordosis angle, and cervical range of motion (ROM). There was no statistically significant difference in the postoperative severity or recovery rate of myelopathy between the two groups. The amount of blood loss during surgery was significantly less in the laminoplasty group. Donor site pain and neck pain was minimal in all patients. Cervical lordosis angle and ROM were diminished postoperatively without a significant difference between the two groups. CONCLUSIONS: There was no critical difference between the ADF and laminoplasty groups with regard to neurological recovery and other surgery-related factors 1 year after surgery. Laminoplasty can be employed for cervical myelopathy caused by soft disc herniation in particular combined with multilevel spinal canal stenosis to avoid secondary myelopathy.


Subject(s)
Arthroplasty , Cervical Vertebrae/surgery , Decompression, Surgical , Intervertebral Disc Displacement/surgery , Spinal Fusion , Adult , Aged , Female , Follow-Up Studies , Humans , Intervertebral Disc Displacement/diagnosis , Magnetic Resonance Imaging , Male , Middle Aged , Range of Motion, Articular , Recovery of Function
16.
J Orthop Sci ; 15(1): 97-103, 2010 Jan.
Article in English | MEDLINE | ID: mdl-20151258

ABSTRACT

BACKGROUND: Cervical myelopathy is more common among Japanese than Westerners. The shorter anteroposterior diameter of the cervical spinal canals (AP diameter) is its probable cause. In recent years, builds of younger Japanese have become larger and been approaching those of Westerners. The purpose of this study was to investigate whether the cervical spinal canal had enlarged in the younger Japanese as well as any cross-sectional improvement in their builds. METHODS: The subjects included 300 men and 300 women who were healthy and without symptoms related to the cervical spine. They were divided into six age groups at 10-year intervals from the twenties to the seventies. Height, body weight, and arm span were measured as physical factors. Using lateral dynamic radiographs of the cervical spine, the AP diameter from C3 to C6 in the neutral position and Penning's jaw diameter in extension (jaw diameter) from C2/3 to C5/6 were measured. The number of trapezoid-shaped vertebral bodies with a thickened posterior margin were also counted as such thickening might be one of the causes of spinal canal narrowing. Statistical analysis was performed for the following associations in both sexes: (1) age and physical factors; (2) age and the AP diameter; (3) age and jaw diameter; and (4) the difference of the AP diameter of the canal within and outside the trapezoid-shaped deformity of the vertebral body. RESULTS: In both men and women, the younger generations statistically had a larger height, arm span, and AP diameter. Older generations showed a significantly narrower jaw diameter at all measured spinal levels in both sexes. Trapezoid-shaped vertebral bodies were found in 3.5% of the men and in 1.3% of the women in their fifties, sixties, and seventies, which statistically had no effect on the AP diameter being wider in the younger generations. CONCLUSIONS: Younger generations had larger builds and a wider canal of the cervical spine. A narrow spinal canal is a fundamental risk factor for cervical myelopathy. Therefore, cervical myelopathy might be expected to decrease in Japan in the near future when the present younger generations have aged.


Subject(s)
Aging , Anthropometry , Cervical Vertebrae/anatomy & histology , Spinal Canal/anatomy & histology , Adult , Aged , Female , Humans , Japan , Male , Middle Aged , Reference Values , Sex Distribution , Young Adult
17.
Ups J Med Sci ; 114(4): 242-8, 2009.
Article in English | MEDLINE | ID: mdl-19961269

ABSTRACT

BACKGROUND: Low-intensity pulsed ultrasound (LIPUS) is reported to have the effects of rapid appearance and early maturation of ossification in animal models. METHOD: We examined the influence of LIPUS on bone formation in C57BL/6J mouse muscle induced by gene transfer of BMP-4 expression plasmid. Electroporation was employed to transfer plasmid DNA. First, an in vitro study was carried out to confirm that LIPUS has no effect on the forced expression of BMP-4 gene transferred by electroporation into C2C12 cells. Next, the BMP-4 plasmids were injected into mouse calf muscles, and transcutaneous electroporation was applied. LIPUS (30 mW/cm(2)) exposure was performed daily for 20 minutes on one side of hind limbs (LIPUS side). The contralateral limbs were not exposed to LIPUS (control side). Muscle samples were collected at 7, 10, 14, and 21 days after electroporation. Soft X-ray films of muscles were taken, and areas of bone formation were measured. After pepsin solubilization of the muscles, calcium and total collagen content were measured. RESULTS: Radiographical measurements showed significantly more bone formation in the LIPUS side at Day 10. The area of bone was the maximum in both sides at Day 14. The LIPUS side exhibited significant increase in the calcium content at Day 10. The total collagen content with LIPUS exposure was increased significantly over control at Day 10 and 21. CONCLUSIONS: According to these results, accelerated maturation of ectopic bone formation by LIPUS was confirmed at Day 10. Moreover, our results showed that LIPUS increases the total collagen content during osteogenesis.


Subject(s)
Muscle, Skeletal/metabolism , Osteogenesis , Ultrasonic Therapy/methods , Animals , Base Sequence , Bone Morphogenetic Protein 4/genetics , Calcium/metabolism , Cell Line , Collagen/metabolism , DNA Primers/genetics , Electroporation , Fracture Healing , Gene Expression , Gene Transfer Techniques , Male , Mice , Mice, Inbred C57BL , Ossification, Heterotopic , Recombinant Proteins/genetics
18.
Histopathology ; 55(4): 432-40, 2009 Oct.
Article in English | MEDLINE | ID: mdl-19817894

ABSTRACT

AIMS: Fibroblast activation protein (FAP)/seprase and dipeptidylpeptidase-IV (DPP-IV)/CD26 are serine integral membrane proteases. They are involved in tissue remodelling, cancer invasion and metastases, mechanisms that are controversial. The aim was to identify cell types that express FAP and DPP-IV in human bone and soft tissue tumours, and to determine whether there are any correlations between the expression of FAP and DPP-IV and the malignant potential of tumours. METHODS AND RESULTS: This study analysed in situ expression in 25 malignant and 13 benign human bone and soft tissue tumours. Reverse transcriptase-polymerase chain reaction analyses confirmed mRNA expression of FAP and DPP-IV in all individuals. Immunohistochemistry using pre-fixed frozen sections revealed that FAP was positive in low-grade myofibroblastic sarcoma, the fibroblastic component of osteosarcomas, and malignant fibrous histiocytomas, but negative in Ewing's sarcomas and rhabdomyosarcomas. DPP-IV showed similar immunohistochemical results. Among benign tumours, non-ossifying fibromas, desmoid tumours and chondroblastomas expressed both FAP and DPP-IV. Giant cells expressed DPP-IV in giant cell tumours. CONCLUSIONS: Our data suggest that FAP and DPP-IV are consistently expressed in bone and soft tissue tumour cells that are histogenetically related to activated fibroblasts and/or myofibroblasts, irrespective of their malignancy. DPP-IV is also expressed in monocyte-macrophage lineage cells.


Subject(s)
Biomarkers, Tumor/metabolism , Bone Neoplasms/metabolism , Bone Neoplasms/pathology , Dipeptidyl Peptidase 4/metabolism , Gelatinases/metabolism , Membrane Proteins/metabolism , Serine Endopeptidases/metabolism , Soft Tissue Neoplasms/metabolism , Soft Tissue Neoplasms/pathology , Endopeptidases , Fibroblasts/metabolism , Fibroblasts/pathology , Histiocytoma, Malignant Fibrous/metabolism , Histiocytoma, Malignant Fibrous/pathology , Humans , Macrophages/metabolism , Macrophages/pathology , Monocytes/metabolism , Monocytes/pathology , Osteosarcoma/metabolism , Osteosarcoma/pathology , Rhabdomyosarcoma/metabolism , Rhabdomyosarcoma/pathology , Sarcoma, Ewing/metabolism , Sarcoma, Ewing/pathology
20.
J Neurosurg Spine ; 9(1): 62-6, 2008 Jul.
Article in English | MEDLINE | ID: mdl-18590413

ABSTRACT

The authors report a rare case of tethered cord syndrome with low-placed conus medullaris complicated by a vertebral fracture that was successfully treated by a spine-shortening vertebral osteotomy. The patient was a 57-year-old woman whose neurological condition worsened after a T-12 vertebral fracture because a fracture fragment and the associated local kyphotic deformity directly compressed the tethered spinal cord. An osteotomy of the T-12 vertebra was performed in order to correct the kyphosis, remove the fracture fragment, and reduce the tension on the spinal cord. Postoperative radiographs showed the spine to be shortened by 22 mm, and the kyphosis between T-11 and L-1 improved from 23 degrees to 0 degrees . Two years after the surgery, the patient's neurological symptoms were resolved. The bone union was complete with no loss of correction.


Subject(s)
Neural Tube Defects/complications , Neural Tube Defects/surgery , Osteotomy , Spinal Fractures/complications , Spinal Fractures/surgery , Thoracic Vertebrae/surgery , Female , Humans , Kyphosis/etiology , Kyphosis/surgery , Middle Aged
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