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1.
Genome ; 54(9): 710-7, 2011 Sep.
Article in English | MEDLINE | ID: mdl-21848446

ABSTRACT

The chromosomal organization of two novel repetitive DNA sequences isolated from the Chenopodium quinoa Willd. genome was analyzed across the genomes of selected Chenopodium species. Fluorescence in situ hybridization (FISH) analysis with the repetitive DNA clone 18-24J in the closely related allotetraploids C. quinoa and Chenopodium berlandieri Moq. (2n = 4x = 36) evidenced hybridization signals that were mainly present on 18 chromosomes; however, in the allohexaploid Chenopodium album L. (2n = 6x = 54), cross-hybridization was observed on all of the chromosomes. In situ hybridization with rRNA gene probes indicated that during the evolution of polyploidy, the chenopods lost some of their rDNA loci. Reprobing with rDNA indicated that in the subgenome labeled with 18-24J, one 35S rRNA locus and at least half of the 5S rDNA loci were present. A second analyzed sequence, 12-13P, localized exclusively in pericentromeric regions of each chromosome of C. quinoa and related species. The intensity of the FISH signals differed considerably among chromosomes. The pattern observed on C. quinoa chromosomes after FISH with 12-13P was very similar to GISH results, suggesting that the 12-13P sequence constitutes a major part of the repetitive DNA of C. quinoa.


Subject(s)
Chenopodium quinoa/genetics , Chromosomes, Plant/genetics , RNA, Ribosomal/genetics , Repetitive Sequences, Nucleic Acid/genetics , Base Sequence , DNA, Plant/genetics , DNA, Ribosomal/genetics , Gene Library , Genome, Plant , In Situ Hybridization, Fluorescence , RNA, Ribosomal/analysis , Sequence Analysis, DNA
2.
Genome ; 49(7): 825-39, 2006 Jul.
Article in English | MEDLINE | ID: mdl-16936791

ABSTRACT

The nucleolus organizer region (NOR) and 5S ribosomal RNA (rRNA) genes are valuable as chromosome landmarks and in evolutionary studies. The NOR intergenic spacers (IGS) and 5S rRNA nontranscribed spacers (NTS) were PCR-amplified and sequenced from 5 cultivars of the Andean grain crop quinoa (Chenopodium quinoa Willd., 2n = 4x = 36) and a related wild ancestor (C. berlandieri Moq. subsp. zschackei (Murr) A. Zobel, 2n = 4x = 36). Length heterogeneity observed in the IGS resulted from copy number difference in subrepeat elements, small re arrangements, and species-specific indels, though the general sequence composition of the 2 species was highly similar. Fifteen of the 41 sequence polymorphisms identified among the C. quinoa lines were synapomorphic and clearly differentiated the highland and lowland ecotypes. Analysis of the NTS sequences revealed 2 basic NTS sequence classes that likely originated from the 2 allopolyploid subgenomes of C. quinoa. Fluorescence in situ hybridization (FISH) analysis showed that C. quinoa possesses an interstitial and a terminal pair of 5S rRNA loci and only 1 pair of NOR, suggesting a reduction in the number of rRNA loci during the evolution of this species. C. berlandieri exhibited variation in both NOR and 5S rRNA loci without changes in ploidy.


Subject(s)
Chenopodium/genetics , DNA, Ribosomal Spacer/genetics , Genes, Plant , Polymorphism, Genetic , RNA, Ribosomal/genetics , Base Sequence , Chenopodium quinoa/genetics , Evolution, Molecular , In Situ Hybridization, Fluorescence , Molecular Sequence Data , Nucleolus Organizer Region , Phylogeny , RNA, Ribosomal, 5S/genetics , Sequence Alignment
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