ABSTRACT
The use of reversed-phase high-performance liquid chromatography for the determination of the biologically active plant phenolic compounds mangiferin, likviritin and dihydroquercetin is described. Perchloric acid (35%) was used for deproteinization in the case of mangiferin and likviritin, and acidified methanol for dihydroquercetin. Detection was performed at 254, 275 and 290 nm for mangiferin, likviritin and dihydroquercetin in plasma, and 365, 312 and 290 nm in urine, respectively. The limit of detection was 0.2 micrograms/ml for plasma and 0.5 micrograms/ml for urine.
Subject(s)
Anti-Inflammatory Agents, Non-Steroidal/pharmacokinetics , Antiviral Agents/pharmacokinetics , Flavonoids/pharmacokinetics , Quercetin/analogs & derivatives , Xanthenes/pharmacokinetics , Xanthones , Animals , Anti-Inflammatory Agents, Non-Steroidal/blood , Anti-Inflammatory Agents, Non-Steroidal/urine , Antiviral Agents/blood , Antiviral Agents/urine , Chromatography, High Pressure Liquid , Flavanones , Flavonoids/blood , Flavonoids/urine , Flavonols , Glucosides , Quercetin/blood , Quercetin/pharmacokinetics , Quercetin/urine , Rats , Reproducibility of Results , Spectrophotometry, Ultraviolet , Xanthenes/blood , Xanthenes/urineABSTRACT
The use of a small precolumn instead of an injection loop for the determination of a new phytochemical drug, fellavine, and its metabolites is described. The method combines the direct injection of plasma and urine into the reversed-phase precolumn with separation on a Spheri-5 RP-18 analytical column. Different sorbents in the precolumn were compared. A recovery of fellavine and its metabolites from biological fluids except rat plasma of almost 100% was achieved on Chrompack RP (30-40 microns) and LiChrosorb RP-18 (7 microns). For rat plasma only the last sorbent gave 80% fellavine recovery. The influence of the protein binding on the fellavine recovery was examined. The limit of detection was equal to 0.05 micrograms/ml fellavine for plasma and 0.02 micrograms/ml for urine. To enhance the limit of detection longer precolumns were perferred.
