ENZYMATIC ACTIVITY IN MICROSOMES, LIPID PEROXIDATION OF MICE HEPATOCYTES UNDER THE SODIUM FLUORIDE.

Fluorine is one of the most widespread and necessary microelements for the body of animals and humans, which is necessary in a clearly limited amount. Different concentrations of fluorine can affect the state of lipid peroxidation, as well as the functional state of the microsomes of liver hepatocytes. The studies were carried out on mature Wistar rats weighing 180-220 g. Animals were inoculated with an aqueous solution of sodium fluoride once a day for 60 days at doses of 1/10, 1/100 and 1/1000 DL50, which was 20 mg/kg, 2 mg/kg and 0,2 mg/kg body weight. Control rats received drinking water. Each group consisted of 10 animals, the studies of indicators were carried out on days 10, 20, 30, 50 and 60. The induction of free radical processes by sodium fluoride was confirmed using a chemiluminescent reaction of blood serum, the amount of diene conjugates in rat liver tissue homogenates was assessed spectrophotometrically, the content of TBA reactants in rat liver tissue homogenates was determined by the reaction of malondialdehyde and thiobarbituric acid (TBA). The level of chiff bases was determined with a spectrofluorometer, subcellular fractions of the liver were isolated by the method of differentiated centrifugation. An increase in the level of the indicator at a dose of 1/10 and 1/100 DL50 of the intensity of lemiluminescence on the 30th day and its decrease on the 60th day was established.Increase in the activity of NAD (P) H-cytochrome c reductase in the microsomal fraction of the liver at the beginning of the study and a gradual decrease on the 50th and 60th days when using both dosages. The same dynamics was observed for NADH-cytochrome c reductase. With respect to diene conjugates of TBA-reactants and chiff bases, a tendency to increase was observed at all periods of the experiment. Indicators of cytochrome P-450 cytochrome b5 were increased up to 30 days and gradually decreased by 60 days. Long-term administration of sodium fluoride can cause the formation of toxic products and a decrease in the activity of enzymes of the microsomal membrane of hepatocytes.

CONTENT OF CATECHOLAMINES IN BLOOD SERUM OF RATS UNDER FLUORIDE INTOXICATION.

The aim of the research was to determine in the experiment the content of catecholamines in serum of rats exposed to sodium fluoride. The studies were conducted on adult Wistar rats, subjected to oral exposure by means of a probe with aqueous solutions of sodium fluoride (SF) once daily, for 60 days at doses of 1/10, 1/100 and 1/1000 DL50, which correspondingly amounts to 20 mg/kg, 2 mg/kg and 0.2 mg/kg of body weight. Toxification of rats at a dose of 1/100 DL50 for 60 days and at a dose of 1/10 DL50 for 50 days was accompanied by an increase in blood levels of norepinephrine and epinephrine, indicating the hyperactivation of the mediator and hormonal parts of the sympathoadrenal system, and tension of the protective and adaptive reactions of the organism. Prolonged hypercatecholemia may become a pathogenic factor due to intensification of the quinidine route of oxidation of norepinephrine and epinephrine with the formation of reactive radicals and active forms of oxygen. Reduced serum content of catecholamines on the 60th day of oral administration at a dose of 1/10 DL50 reflects, on the one hand, a decrease in their tissue deposit, and, on the other, a decrease in the activity and reserve capacities of the sympathoadrenal system.

NITRIC OXIDE SYNTHESIS INTENSITY ASSESSMENT BY THE CONTENT OF ITS TERMINAL STABLE METABOLITES IN THE BLOOD OF RATS UNDER FLUORIDE INTOXICATION.

The aim of the research was to evaluate the intensity of nitric oxide synthesis in the experiment by the content of its terminal stable metabolites in the blood of rats exposed to sodium fluoride. The studies were conducted on adult Wistar rats weighing 180-220 g, subjected to oral exposure by means of a probe with aqueous solutions of sodium fluoride (SF) daily for 60 days at a dose of 1/10, 1/100 and 1/1000 DL50, respectively, of 20 mg/kg, 2 mg/kg and 0.2 mg/kg body weight. Oral administration of SF to rats at doses of 1/10 and 1/100 of DL50 leads to an increase in blood plasma levels of nitrite and nitrate anions during the first 30 days, indirectly indicating the excessive production of nitric oxide, which in the initial period of intoxication can perform compensatory role, but in the future can cause pathological reactions associated with the activation of oxidative stress. The reduction of nitrite and nitrate anions at the end of the long-term effects of SF indirectly indicates a decrease in the generation of nitric oxide, which may be due, in particular, to the increase in the concentration of peroxynitrite as a result of the use of nitric oxide in reaction with a superoxide anion radical and a deficiency of antioxidant enzymes.