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Vet Parasitol ; 183(3-4): 382-5, 2012 Feb 10.
Article in English | MEDLINE | ID: mdl-22055612

ABSTRACT

Native cathepsin-L cysteine proteinase (28 kDa) was purified from the excretory secretory products of Fasciola gigantica and was used for sero-diagnosis of F. gigantica infection in buffaloes by Dot-enzyme-linked immunosorbent assay (Dot-ELISA). The test detected F. gigantica field infection in these animals with a sensitivity of ∼ 90%. No specific IgG antibody binding was displayed by sera obtained from 76 buffaloes considered to be Fasciola and other parasite-free by microscopic examination of faeces and necropsy examination of liver, rumen and intestine. Additionally, sera from 156 Fasciola-free buffaloes, yet infected with Gigantocotyle explanatum, Paramphistomum epiclitum, Gastrothylax spp., Strongyloides papillosus and hydatid cyst were all negative, indicating that F. gigantica cathepsin-L cysteine proteinase does not cross-react with these helminth parasites in natural infection of the host. The data indicated that cathepsin-L cysteine proteinase based Dot-ELISA reached ∼ 90% sensitivity and 100% specificity with relation to above parasites in the detection of bubaline fasciolosis. The present Dot-ELISA diagnostic assay is relevant to the field diagnosis of F. gigantica infection in buffaloes.


Subject(s)
Buffaloes , Cathepsins/immunology , Cysteine Endopeptidases/immunology , Cysteine Proteases/immunology , Enzyme-Linked Immunosorbent Assay/methods , Fasciola/isolation & purification , Fascioliasis/veterinary , Helminth Proteins/immunology , Animals , Antibodies, Helminth/blood , Antigens, Helminth/immunology , Antigens, Helminth/isolation & purification , Cathepsins/isolation & purification , Cysteine Endopeptidases/isolation & purification , Cysteine Proteases/isolation & purification , Enzyme-Linked Immunosorbent Assay/veterinary , Fasciola/enzymology , Fascioliasis/diagnosis , Fascioliasis/immunology , Fascioliasis/parasitology , Feces/parasitology , Helminth Proteins/isolation & purification , Immunoglobulin G/blood , Sensitivity and Specificity , Serologic Tests
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