ABSTRACT
A standard, highly specific and sensitive enzyme immunoassay test system has been developed for the diagnosis of acute opisthorchiasis, which is based on indirect solid-phase enzyme immunoassay for determination IgM antibodies to the antigen derived from Opisthorchis felineus marita extracts. The sensitivity of the test system is 87%, its specificity is 96.8%.
Subject(s)
Opisthorchiasis/diagnosis , Acute Disease , Animals , Antigens, Helminth/immunology , Immunoenzyme Techniques/methods , Immunoglobulin M , Opisthorchis/immunology , Opisthorchis/isolation & purificationABSTRACT
An assay was developed to determine IgM antibodies to Opisthorchis felineus antigens for the diagnosis of acute infection. The method is based on indirect solid-phase enzyme immunoassay. Optimal conditions were elaborated for analysis and its high specificity and specifications are shown in the paper.
Subject(s)
Antibodies, Helminth/blood , Antigens, Helminth/immunology , Immunoglobulin M/blood , Opisthorchiasis/diagnosis , Opisthorchis/immunology , Acute Disease , Animals , Humans , Immunoenzyme Techniques , Sensitivity and SpecificityABSTRACT
Horse serum albumin has been shown to meet the requirements to protein preparations for microanalysis and thus to be suitable for use in kits of reagents for the radioimmunological determination of insulin and myoglobin, for the determination of tick-borne encephalitis virus antigen by the method of the enzyme immunoassay and for the stabilization of proteins in the hemagglutination test and the hemagglutination inhibition test.
