ABSTRACT
We analyzed the expression of galectin-1 and galectin-3 in tumor tissue in stomach and colorectal cancer with and without tissue eosinophilia. Low expression of galectin-3 was detected in all patients with malignant gastrointestinal tumors irrespective of the presence of eosinophilia. Low expression of galectin-1 was detected only in patients with gastrointestinal cancer associated with eosinophilia. Association of galectin-1 expression with eosinophilic infiltration of the tumor tissue in stomach and colorectal cancer was detected.
Subject(s)
Colorectal Neoplasms/metabolism , Eosinophilia/metabolism , Galectin 1/metabolism , Galectin 3/metabolism , Aged , Blood Proteins , Chemotaxis, Leukocyte/physiology , Cohort Studies , Colorectal Neoplasms/complications , Colorectal Neoplasms/pathology , Eosinophilia/complications , Eosinophilia/pathology , Eosinophils/metabolism , Eosinophils/pathology , Galectins , Gastric Mucosa/metabolism , Humans , Middle Aged , Stomach/pathology , Stomach Neoplasms/metabolism , Stomach Neoplasms/pathologyABSTRACT
We studied possible mechanisms of immunosuppression mediated by regulatory T cells that promotes suppression of antigen-specific immune response to Mycobacterium tuberculosis in patients with pulmonary tuberculosis and eosinophilia. It was shown that the number of CD4(+)CD25(+)Foxp3(+) regulatory T cells with immunosuppressive activity (Treg) increased in the peripheral blood of patients with disseminated destructive forms of pulmonary tuberculosis with multiple resistance of the causative agent to antituberculosis substances and eosinophilia. These changes were accompanied by imbalance in secretion of Treg-associated cytokines (in vitro) manifested in hyperproduction of TGFß and IL-10 and decreased production of IL-2.
Subject(s)
Eosinophilia/immunology , Eosinophilia/microbiology , T-Lymphocytes, Regulatory/metabolism , Tuberculosis, Pulmonary/immunology , Tuberculosis, Pulmonary/microbiology , Adolescent , Adult , CD4-Positive T-Lymphocytes/metabolism , Eosinophilia/metabolism , Female , Forkhead Transcription Factors/metabolism , Humans , Interleukin-10/metabolism , Interleukin-2/metabolism , Interleukin-2 Receptor alpha Subunit/metabolism , Male , Middle Aged , Mycobacterium tuberculosis/immunology , Mycobacterium tuberculosis/pathogenicity , Transforming Growth Factor beta/metabolism , Tuberculosis, Pulmonary/metabolism , Young AdultABSTRACT
The parameters characterizing antituberculous immune response at the stage of cytokinedependent activation of T cells were analyzed in 90 patients with pulmonary tuberculosis before etiotropic therapy. Immunophenotyping of T cells for IL-12Rß2, WSX-1, and gp130 surface markers and T-bet intracellular transcription factor was carried out after specific IL-12/IL-27 induction of cells in vitro. An increase in the counts of CD3(+)T-bet(+), CD3(+)IL-12Rß2 (+), and CD3(+)WSX-1(+)gp130(+) cells and the level of T cells with high expression of WSX-1 inhibitory molecule (CD3(+)WSX-1(hi+)gp130(-) and CD3(+)WSX-1(hi+)gp130(+)) was detected. The type of disorders in the inductive stage of antituberculous immune response did not depend on the clinical form of disease.
Subject(s)
Cytokines/metabolism , T-Lymphocytes/metabolism , Tuberculosis, Pulmonary/metabolism , Adult , Female , Humans , Immunophenotyping , Interleukins/metabolism , Male , Middle Aged , Young AdultABSTRACT
In patients with infiltrative pulmonary tuberculosis, the increase in IL-6 secretion, the decrease in TGFß production (in case of drug resistance of the causative agent), and unchanged level of IL-1ß secretion by mononuclear leukocytes in vitro were associated with increased number of CD4(+)CD161(+)IL-17A(+) Th17 lymphocytes in the peripheral blood. In patients with disseminated drug-resistant pulmonary tuberculosis, TGFß hyperproduction promoted differentiation of CD4(+)CD25(+)FoxP3(+) Treg lymphocytes with immunosuppressive activity.
Subject(s)
Cell Differentiation/immunology , T-Lymphocytes, Regulatory/immunology , Th17 Cells/immunology , Tuberculosis, Pulmonary/immunology , Adult , CD4 Antigens/metabolism , Female , Forkhead Transcription Factors/metabolism , Humans , Interleukin-2 Receptor alpha Subunit/metabolism , Male , Middle Aged , NK Cell Lectin-Like Receptor Subfamily B/metabolism , Statistics, Nonparametric , T-Lymphocytes, Regulatory/metabolism , Th17 Cells/metabolism , Transforming Growth Factor beta/immunology , Tuberculosis, Pulmonary/bloodABSTRACT
Increased content of CD4(+)CD161(+)IL-17A(+) Th17 lymphocytes in the peripheral blood was found in patients with pulmonary tuberculosis irrespective of the clinical form (infiltrative, disseminated) and variant of the disease (drug-sensitive, drug-resistant). The elevated content of Th17 cells in pulmonary tuberculosis is associated with hypersecretion of Th17-associated cytokines IL-17A and IL-22 in vitro that was most pronounced (in case of IL-17A) in patients with disseminated pulmonary tuberculosis.
Subject(s)
Interleukin-17/metabolism , Interleukins/metabolism , Mycobacterium tuberculosis/immunology , Th17 Cells/immunology , Tuberculosis, Pulmonary/immunology , Adult , CD4 Antigens/metabolism , Cells, Cultured , Female , Humans , Male , Middle Aged , NK Cell Lectin-Like Receptor Subfamily B/metabolism , Young Adult , Interleukin-22ABSTRACT
We studied in vitro effects of recombinant interleukin-5, interleukin-3, and eotaxin on programmed death of eosinophils from healthy donors and patients with non-Hodgkin's lymphomas associated with severe blood eosinophilia. Interleukin-5 and eotaxin produced the most potent antiapoptotic effect on eosinophils from healthy donors. In patients with non-Hodgkin's lymphomas, spontaneous apoptosis in eosinophilic leukocytes was low and remained unchanged during incubation with recombinant proteins.
