[The reference intervals of main parameters of urine for analyzers SYSMEX UF 1000I/500I in dynamics of uncomplicated pregnancy.]

The analyzer Sysmex UF1000i/500i were used to measure 8 parameters of urine of pregnant women; the upper reference values were formed. The women (n=158) with uncomplicated course of pregnancy were examined. No calculation of reference intervals was applied to leukocytes, erythrocytes, pavement and transitional/renal epithelium, cylinders (pathological ones included) due to their correspondence to similar values for non-pregnant women. for indices of bacteria and crystals proper upper reference thresholds were calculated that in trimesters I, II and III amounted to 452,8, 687,9 и 571,4 u/mkl for bacteria, and 0,59, 0,92 и 0,7 u/mkl for salts correspondingly.

[The placental growth factor and fms-similar tyrosine kinase-1 as markers of pre-eclampsy in dynamics of pregnancy].

The article presents reference limits of concentrations of placental growth factor and fms-similar tyrosine kinase-1 and values of their ratio in dynamics of physiologic pregnancy and in female patients with pre-eclampsy of different degree of severity in 1 and III trimesters of pregnancy. It is established that in female patients with pre-eclampsy ratio of fms-similar tyrosine kinase-1 and placental growth factor significantly exceed ratio in female patients with physiological course of pregnancy. The degree of increase of values of ration between fms-similar tyrosine kinase-1 and placental growth factor correlates with degree of severity of pre-eclampsy.

[Pharmacokinetics of mifepristone and kinetics of chorionic gonadotropin beta-subunit (beta-HCG) and progesterone in plasma of pregnant women during pharmacological interruption of early pregnancy]. / Farmakinetika mifepristona i kinetika beta-sub''edinitsy khorionicheskogo gonadotropina (beta-KhG) i progesterona v plazme krovi beremennykh zhenshchin pri medikamentoznom preryvanii rannei beremennosti.

Reliable correlations between the parameters of mifepriston pharmacokinetics describing the rate of drug elimination from the blood plasma and the levels of beta-human chorionic gonadotropin (beta-HCG) and progesterone reflecting the state of gestation in females have been established fore the first time. According to these relationships, the half elimination time, the mean retention time, and the plasma clearance of mifepriston can be considered as predictors of the clinical efficacy of this drug for the early pregnancy interruption.

[Comparative analysis of serum analyte level measurements by commercial diagnostic kits from four manufacturers (Alkor-Bio, Roche, Diagnostic Products Corporation-DCP, and Bayer Corporation)]. / Sravnitel'nyi analiz opredeleniia kontsentratsii riada analitov v syvorotke krovi s ispol'zovaniem kommercheski dostupnykh naborov reagentov chetyrekh firm-proizvoditelei ("Alkor Bio", "Roche", "Diagnostic Products Corporation"-DCP i "Bayer Corporation").

Hydrocortisone, progesterone, testosterone, triiodothyronine, thyroxine, chorionic gonadotropin, prolactin, alpha-fetoprotein, luteinizing, follicle-stimulating, and thyrotropic hormones were measured in human sera and in Lyphochek Immunoassay Plus Control reference sera (Bio-Rad Laboratories, USA) using 4 commercial kits (Alkor Bio Inc. and Roche, automated analyzer Roche Cobas Core; DPC, automated analyzer Immulite; Bayer, automated analyzer ACS:180). Coordination and correlation between these kits was observed, the coordination decreasing in the series Alkor Bio/Bayer, Alkor Bio/Roche, and Alkor Bio/DPC.

[Hypogonadotropic form of amenorrhea. Features of the functional state of hypothalamo-hypophyseal structures and the endocrine status]. / Gipogonadotropnaia forma amenorei. Osobennosti funktsional'nogo sostoianiia gipotalamo-gipofizarnykh struktur i éndokrinnyi status.

Study of the function of the hypothalamopituitary system in patients with hypogonadotrophic amenorrhea showed the activity of its structures which manifested by the presence of pulsed LH secretion in 70% of women; however, the parameters of pulsed secretion of gonadotropins were disordered: the pulses were chaotic and low-amplitude. Adenohypophyseal gonadotrophs were capable of reacting to the administered gonadotrophin-releasing hormone, but the parameters of this reaction differed from the normal. The endocrine status of patients with hypogonadotrophic amenorrhea was changed vs. that in health: prolactin level was reduced, growth hormone level increased, thyroid and adrenocortical function changed; the pattern of secretion of some adenohypophyseal trophic hormones (prolactin, STH, TTH) was also changed. The results permit a hypothesis that impairment of the common central mechanisms regulating the endocrine system underlies the development of this disease; basing on their findings, the authors propose new approaches to the correction of this abnormality.

[Hypogonadotropic form of amenorrhea. Principles of ovulation induction]. / Gipogonadotropnaia forma amenorei. Printsipy induktsii ovuliatsii.

Analysis of the clinico-anamnestic and endocrine parameters of the reproductive system of 45 patients with hypogonadotropic amenorrhea helped single out three types of this condition. Drug doses and schemes of their administration to induce ovulation were selected individually with due consideration for the initial functional status of the reproductive system. The authors defined the basic principles of ovulation induction in patients with hypogonadotropic amenorrhea: the patients should be carefully selected according to WHO classification, with due regard for their clinico-anamnestic data and the function of the reproductive system (hormonal functional test); drug doses for substitution therapy and protocols of their administration should be selected individually, with consideration for the degree of hypophyseal-gonadal insufficiency; daily double (ultrasonic and hormonal) monitoring is needed for the correction of ovulation induction protocols; the choice of the optimal time of administration of the "ovulatory" dose should be based on the findings of double monitoring indicating follicle size 19-20 mm and the maximal activity of steroidogenesis (350 to 400 pmol/liter estradiol per follicle). The possibility of using lutrelef, an analog of gonadotropin releasing hormone, for ovulation induction in patients with the hypothalamic form of gonadotropic amenorrhea was studied. The drug was administered in a pulsed mode using Zykloma+ device (Ferring, Germany). The advantages of a physiological principle of substitution therapy were demonstrated, although the induction of cycles was not appreciably improved by this method as against substitution therapy with human menopausal gonadotropin.

[Functional state of the reproductive system in patients with the hypogonadotropic form of amenorrhea during use of various protocols of ovulation induction with human menopausal gonadotropins]. / Osobennosti funktsional'nogo sostoianiie reproduktivnoi sistemy patsientok s gipogonadotropnoi formoi amenorei pri ispol'zovanii razlichnykh skhem induktsii ovuliatsii chelovecheskimi menopausal'nymi gonadotropinami.

Clinico-hormonal parameters of cycles stimulated with human menopausal gonadotropins in patients with hypogonadotropic amenorrhea and of spontaneous cycles in healthy women of reproductive age are compared. Estradiol hypersecretion in both phases of induced cycle was revealed: hydrocortisone in phase 1 and progesterone and testosterone in phase 2. Dexamethasone in daily dose 0.25 mg was administered for correction. This resulted in a noticeable reduction of estradiol hyperproduction in the first phase of stimulated cycles and in hyperandrogenism elimination. Folliculogenesis, ovulation, and early embryogenesis conditions in schemes of induction with human menopausal gonadotropins and dexamethasone therapy were close to physiologic ones, this resulting in increased share of fertile cycles.

[Cortisol determination in human plasma by a modified method of competitive protein binding]. / Opredelenie kortizola v plazme krovi cheloveka modifitsirovannym metodom konkurentnogo sviazyvaniia s belkami.

The authors described a simple and economic modification of the competitive protein binding method of cortisol determination in human blood plasma satisfying the current requirements presented to the saturation analysis. Blood plasma of women given combined contraceptive the preparation was used as a binding system. Analysis of experimental results using a programmed computer was carried out on the basis of standard curve linearization. Preeision sensitivity and specificity of this modified method are assessed. This modification was approved in measuring the cortisol level in the blood plasma of various groups of patients. Comparison with routine competitive binding methods and their analysis is presented.