The use of interferon for emergency prophylaxis of marburg hemorrhagic fever in monkeys.

The possibility of emergency prophylaxis of Marburg hemorrhagic fever with leukocytic and recombinant interferons was studied in experiments on Cercopithecus aethiops. None of the agents protected monkeys from the action of lethal doses of Marburg virus. Recombinant interferon-alpha(2)administered according to the emergency prophylaxis schedule prolonged the mean life-span of monkeys injected with Marburg virus in doses of 100 and 1000 LD50 by 1.9 and 6.1 days, respectively.

[Study of the antiviral activity, pharmacokinetics and toxic properties of liposomal form of human alpha-2b interferon administered topically]. / Izuchenie protivovirusnoi aktivnosti, farmakokinetiki i toksicheskikh svoistv liposomal'noi formy rekombinantnogo alfa-2b-interferona cheloveka pri naruzhnom primenenii.

Human liposomal recombinant alpha 2b-interferon topically applied to laboratory animals was tested for antiviral activity, pharmacokinetics, and toxicity. The interferon was shown to penetrate through the skin and to circulate in the blood of experimental animals longer than its injectable form, and to exhibit its antiviral activity against genital herpes in guinea pigs. It produced no toxic, skin-irritant, or allergic effects in laboratory animals.

[Design of human liposomal recombinant alpha2-interferon-containing preparation]. / Sozdanie liposomal'nogo preparata, soderzhashchego chelovecheskii rekombinantnyi alpha2-interferon.

The composition of liposomal lipid phase preparation was selected. The optimum ratio of liposomal lipid phase components was found to produce a a human liposomal recombinant alpha 2-interferon-containing preparation by extrusion across the polycarbonate membranes. The study gave rise to a lyophilized sterile liposomal recombinant alpha 2-interferon preparation. The agent was shown to be stable and retains its antiviral activity within a year.

[Methodological approaches to creating a liposomal form of human recombinant alpha 2-interferon]. / Metodichesie podkhody k sozdaniiu liposomal'noi formy chelovecheskogo rekombinantnogo al'fa 2-interferona.

The authors have obtained several variants of liposomal forms of human alpha 2-interferon. The forms intended to preserve antiviral activity with regard to the methods of liposomal formation were comparatively studied. It has been found that liposomal formation through stirring caused no decrease in antiviral activity.

[Immunochemical and biological properties of synthetic peptide fragments from the 124-144 sequence of human leukocyte interferon alpha2]. / Immunokhimicheskie i biologicheskie svoistva sinteticheskikh peptidnykh fragmentov posledovatel'nosti 124-144 leikotsitarnogo interferona cheloveka alpha2.

Three peptides corresponding to the sequences 124-144, 124-138, 129-144 of the human leukocyte interferon alpha 2 (IFN-alpha 2) were synthesized. The synthesis was performed by DCC-HOBT coupling of protected peptide segments in solution. The segments were obtained by the active ester coupling methodology using base-labile 2-[4-(phenylazobenzyl)sulfonyl]ethyl (Pse) group as carboxyterminal protection. After complete deprotection with 1 M methanesulphonic acid in trifluoroacetic acid--thioanisol--m-cresol mixture the peptides were purified by reversed-phase chromatography. The studies of interaction of the peptides with rabbit antiserum against IFN-alpha 2 revealed at least one minor antigenic determinant within the 124-144 region of IFN-alpha 2 amino acid sequence. Rabbit antisera developed against peptides 124-138 and 129-144 showed ability of binding recombinant IFN-alpha 2 and neutralizing its antiviral activity. Free peptides or their conjugates with bovine serum albumine did not display antiviral activity, neither could they inhibit the activity of IFN-alpha 2.

[Comparative study of interferon inducers obtained from natural sources]. / Sravnitel'noe issledovanie induktorov interferona, poluchennykh iz prirodnykh istochnikov.

Interferon-inducing and antiviral effects of natural dsRNA preparations of phage phi 6 and yeast cells were studied in the culture of murine cells L-929 and on random bred albino mice. Both the preparations showed interferon inducing activity in the cell culture. However, for realization of their effect modification of the surface cell membrane by polycation exchange resin (DEAE-dextran) was required. The interferon-inducing activity of both of the natural dsRNA in the mice was high. The maximum interferon titers (1280-5120 units/ml) in blood serum were observed 4-6 hours after the inductor intraperitoneal administration. The interferon-inducing activity of the phage dsRNA was high in the cell culture and yeast dsRNA--in mice, respectively. Both the inductors had antiviral activity and protected 15 to 38.9 per cent of the experimental animals from the effect of 100 LD50 of the murine encephalomyocarditis virus and 10 LD50 of the influenza virus A/Aichi 2/68 (H3N2).