[Morphological changes of stomach innervation after vagotomy]. / Morfologicheskie izmeneniia innervatsionnogo apparata zheludka posle vagotomii.

The morphologic signs of response of the peripheric neural system of the stomach to vagotomy in animals and in patients with the ulcer disease were compared. The innervative apparatus of the stomach was studied in 4 intact dogs (control), 6 animals who underwent selective proximal vagotomy (SPV) and in 13 patients with the ulcer disease reoperated after SPV performance in connection with the development of a recurrent ulcer. Compensatory possibilities of the innervative apparatus of the stomach in human are lower than those in animals.

[Clinico-morphologic basis for choosing the surgical method in the treatment of exstrophy of the bladder]. / Kliniko-morfologicheskoe obosnovanie vybora sposoba khirurgicheskogo lecheniia ékstrofii mochevogo puzyria.

A method for creation of an artificial bladder from the rectum, which was used in 22 children with exstrophy of the bladder with good anatomic and functional results is suggested. In clinical and histologic studies, the inferiority of the exstrophied bladder tissues and unexpediency of their use for plasty have been proved. A long-term result of treatment in 31 of the 35 patients operated on with the use of different methods was studied by the data of complex evaluation of the functions of an artificial bladder, upper urinary tracts and rectal sphincter apparatus.

[Changes in the argyrophil carcass of the lobules of the thymus gland during its age-related involution]. / Izmeneniia argirofil'nogo karkasa dolek vilochkovoi zhelezy pri ee vozrastnoi involiutsii.

Fibrillar components of the thymus lobules have been studied in persons 9-73 years of age and in 11-30 month-old noninbred white rats. Beginning from 9-10 years of age, i. e. before the onset of age involution, in the medullary substance of the lobules argyrophil carcass is formed; it includes numerous microvessels. At the age of 30-40 years a part of argyrophil fibers is subjected to destruction. At the same time in the medullary substance adipocytes appear. Their increasing amount is accompanied with substitution of the medullary substance for adipose substance, while a part of the cortical substance is preserved. These data are confirmed, when the thymus in old animals is studied. Ultrastructural manifestations of extra- and intracellular destruction of fibers are noted in them, too. The dynamics of the fibrillar component changes in the thymus in different animal individuals is not the same and can serve as one of the criteria of its age involution.

[Creation of an artificial smooth muscle rectal sphincter in children]. / Sozdanie iskusstvennogo gladkomyshechnogo sfinktera priamoi kishki u detei.

A method for creation of the artificial smooth muscle anal sphincter, which was used in 18 children with high forms of anorectal atresia and in 9 children with associated incompetence of the external and internal anal sphincters developed after repeated operations, severe traumas of the perineum and rectum and suppurative processes, has been developed. The effectiveness of the developed method is shown on the basis of clinical, functional and experimental morphologic methods of investigation.

[Rapid method of silver nitrate impregnation of elements of the peripheral nervous system suitable for celloidin and paraffin sections]. / Bystryi metod impregnatsii azotnokislym serebrom élementov perifericheskoi nervnoi sistemy, prigodnyi dlia tselloidinovykh i parafinovykh srezov.

According to the method of neural elements impregnation in the authors' modification, the object is fixed for 6-12 h in Lillie fluid cooled to 4 degrees C. Then the object is kept under tap water for 2-6 h. Frozen sections are prepared and kept in pure pyridine for 1-6 h. When the sections are embedded into paraffin or celloidin, they are put into alcohol solutions gradually decreasing their concentration until water is reached, then put into pyridine. In order to remove cellulose, the celloidin sections are treated in 3 portions of pyridine (in the 1st and 2nd-for 10 min, and in the 3d-for 6 h). Then they are washed under tap water for 2-4 h and in distilled water for 30-40 min. Further treatment is performed according to the methods by Bielschowsky - Gros, Kampos or Rasskazova. Excess silver is removed by treating the sections in 2% ammonium persulfate under the microscope control (the process is stopped by putting the sections into 7% sodium hyposulfate for 10 min). Then the sections are treated in 0.1% aurum chloride, in 5% hyposulfite to reveale the tissue background [corrected] and by means of routine histological techniques either after Brashet, Hale, PAS-positive reaction or other methods applied after fixation in Lillie fluid.

[Comparative evaluation of biodestruction of polyurethanes synthetized on the basis of simple and complex polyesters]. / Sravnitel'naia otsenka biodestruktsii poliuretanov, sintezirovannykh na osnove prostykh i slozhnykh poliéfirov.

In model liquids and experimental animals (rats, rabbits) with the aid of physico-mechanical and histological methods (using interferentially polarizating microscopy) a comparative evaluation was made of the biodestruction of polyurethanes from synthetized simple and complex polyesters. The tissue reaction to intra-abdominal, intramuscular and subcutaneous implantation of perforated stripes of A-19 and Nr. 36 polymer was moderate. Two weeks post-operative increased volumetric biodestruction of the polymers was observed. The course was different in various standards and depended upon the chemical structure of the polymers. Taking into account the slight macrophage tissue reaction, in counterdistinction to the significant volumetric biodestruction which envolved the entire width of the polymer strip, it is assumed that the destruction of the investigated polyurethane composition (adhesive KL-3) takes place by hydrolysis 3 to 6 months post-implant.

[Role of macrophages and foreign body type giant cells in the biodestruction process of polyurethane adhesive tissue]. / Rol' makrofagov i gigantskikh kletok inorodnykh tel v protsesse biodestruktsii meditsinskogo poliuretanovogo kleia.

The biodestruction process of polyurethane adhesive was investigated in application to intestine anastomoses, wounds of skeletal muscles and surfaces of some internal organs. The polymer destruction was proved to be connected with the activity of giant cells of foreign body type and macrophages phagocyting the micromolecules of the adhesive. The presence of adhesive microfragments in macrophages and giant cells was ascertained on the basis of a method elaborated by the authors consisting in staining of the polyurethane adhesive with Sudan III-IV. Moreover, it was pointed out, that biodestruction products had no negative effect in the regeneration of nervous and muscle fibers.