1.
Nucleic Acids Res
; 20(14): 3787, 1992 Jul 25.
Article
in English
| MEDLINE
| ID: mdl-1322531
Subject(s)
Acinetobacter calcoaceticus/enzymology , DNA Restriction Enzymes/metabolism , Deoxyribonucleases, Type II Site-Specific/metabolism , Bacteriophage lambda/genetics , Chromatography , DNA Restriction Enzymes/isolation & purification , DNA, Bacterial/metabolism , DNA, Viral/metabolism , Deoxyribonucleases, Type II Site-Specific/isolation & purification , Plasmids/genetics
2.
Nucleic Acids Res
; 20(14): 3789, 1992 Jul 25.
Article
in English
| MEDLINE
| ID: mdl-1322532
Subject(s)
Arthrobacter/enzymology , DNA Restriction Enzymes/metabolism , Deoxyribonucleases, Type II Site-Specific/metabolism , Bacteriophage lambda/genetics , Chromatography , DNA Restriction Enzymes/isolation & purification , DNA, Bacterial/metabolism , DNA, Viral/metabolism , Deoxyribonucleases, Type II Site-Specific/isolation & purification , Plasmids/genetics , T-Phages/genetics
3.
Nucleic Acids Res
; 19(9): 2504, 1991 May 11.
Article
in English
| MEDLINE
| ID: mdl-1645873
4.
Nucleic Acids Res
; 18(19): 5807-10, 1990 Oct 11.
Article
in English
| MEDLINE
| ID: mdl-2216771
ABSTRACT
Unique restriction endonucleases Bpu 10l and Bsil have been isolated from Bacillus pumilas and Bacillus sphaericus, respectively. The recognition sequences and cleavage points of these enzymes have been determinated as 5'-CC1TNAGC-3'/3'-GGANT1CG-5' for Bpu 10l and 5'-C1TCGTG-3'/3'-GAGCA1C-5' for Bsil. Restriction endonucleases Bpu 10l and Bsil represent a new class of enzymes which recognize non-palindromic nucleotide sequences and hydrolize DNA within the recognition sequence. Bpu 10l and Bsil recognition sequences may be regarded as quasipalindromic and the enzymes may be designated as type II-Q restriction endonucleases.