ABSTRACT
Psoralen-conjugated triplex-forming oligonucleotides (Ps-TFOs) have been utilized for genome editing and anti-gene experiments for over thirty years. However, the research on Ps-TFOs employing artificial nucleotides is still limited, and their photo-crosslinking properties have not been thoroughly investigated in relation to biological activities. In this study, we extensively examined the photo-crosslinking properties of Ps-TFOs to provide fundamental insights for future Ps-TFO design. We developed novel Ps-TFOs containing 2'-O,4'-C-methylene-bridged nucleic acids (Ps-LNA-mixmer) and investigated their photo-crosslinking properties using stable cell lines that express firefly luciferase constitutively to evaluate the anti-gene activities of Ps-LNA-mixmer. As a result, Ps-LNA-mixmer successfully demonstrated suppression activity, and we presented the first-ever correlation between photo-crosslinking properties and their activities. Our findings also indicate that the photo-crosslinking process is insufficient under cell irradiation conditions (365â nm, 2â mW/cm2 , 60â min). Therefore, our results highlight the need to develop new psoralen derivatives that are more reactive under cell irradiation conditions.
Subject(s)
Nucleic Acids , Oligonucleotides , Oligonucleotides/pharmacology , Ficusin/pharmacology , DNA/metabolism , Cell LineABSTRACT
The 332-nucleotide small nuclear RNA (snRNA) 7SK is a highly conserved non-coding RNA that regulates transcriptional elongation. By binding with positive transcriptional elongation factor b (P-TEFb) via HEXIM1, 7SK snRNA decreases the kinase activity of P-TEFb and inhibits transcriptional elongation. Additionally, it is reported that 7SK inhibition results in the stimulation of human immunodeficiency virus (HIV)-specific transcription. These reports suggest that 7SK is a naturally occurring functional molecule as negative regulator of P-TEFb and HIV transcription. In this study, we developed functional oligonucleotides that mimic the function of 7SK (7SK mimics) as novel inhibitors of HIV replication. We defined the essential region of 7SK regarding its suppressive effects on transcriptional downregulation using an antisense strategy. Based on the results, we designed 7SK mimics containing the defined region. The inhibitory effects of 7SK mimics on HIV-1 long terminal repeat promoter specific transcription was drastic compared with those of the control mimic molecule. Notably, these effects were found to be more enhanced by co-transfection with Tat-expressing plasmids. From these results, it is indicated that 7SK mimics may have great therapeutic potential for HIV/AIDS treatment.
Subject(s)
Drug Development , RNA, Small Nuclear/pharmacology , Transcription, Genetic/drug effects , tat Gene Products, Human Immunodeficiency Virus/antagonists & inhibitors , Dose-Response Relationship, Drug , Molecular Structure , RNA, Small Nuclear/chemical synthesis , RNA, Small Nuclear/chemistry , Structure-Activity Relationship , Transcription, Genetic/genetics , tat Gene Products, Human Immunodeficiency Virus/geneticsABSTRACT
UNLABELLED: Pediatric acute encephalopathy (AE) was sometimes attributed to virus infection. However, viral infection does not always result in AE. The risk factors for developing infantile AE upon virus infection remain to be determined. Here, we report an infant with AE co-infected with human herpesvirus-6 (HHV-6) and three picornaviruses, including coxsackievirus A6 (CVA6), Enterovirus D68 (EV-D68), and human parechovirus (HPeV). EV-D68 was vertically transmitted to the infant from his mother. CVA6 and HPeV were likely transmitted to the infant at the nursery school. HHV-6 might be re-activated in the patient. It remained undetermined, which pathogen played the central role in the AE pathogenesis. However, active, simultaneous infection of four viruses should have evoked the cytokine storm, leading to the pathogenesis of AE. CONCLUSION: an infant case with active quadruple infection of potentially AE-causing viruses was seldom reported partly because systematic nucleic acid-based laboratory tests on picornaviruses were not common. We propose that simultaneous viral infection may serve as a risk factor for the development of AE.
ABSTRACT
HIV-1 integrase (IN) is an enzyme which is indispensable for the stable infection of host cells because it catalyzes the insertion of viral DNA into the genome and thus is an attractive target for the development of anti-HIV agents. Earlier, we found Vpr-derived peptides with inhibitory activity against HIV-1 IN. These Vpr-derived peptides are originally located in an α-helical region of the parent Vpr protein. Addition of an octa-arginyl group to the inhibitory peptides caused significant inhibition against HIV replication associated with an increase in cell permeability but also relatively high cytotoxicity. In the current study, stapled peptides, a new class of stabilized α-helical peptidomimetics were adopted to enhance the cell permeability of the above lead peptides. A series of stapled peptides, which have a hydrocarbon link formed by a ruthenium-catalyzed ring-closing metathesis reaction between successive turns of α-helix, were designed, synthesized, and evaluated for biological activity. In cell-based assays some of the stapled peptides showed potent anti-HIV activity comparable with that of the original octa-arginine-containing peptide (2) but with lower cytotoxicity. Fluorescent imaging experiments revealed that these stapled peptides are significantly cell permeable, and CD analysis showed they form α-helical structures, whereas the unstapled congeners form ß-sheet structures. The application of this stapling strategy to Vpr-derived IN inhibitory peptides led to a remarkable increase in their potency in cells and a significant reduction of their cytotoxicity.
Subject(s)
HIV Integrase Inhibitors/chemistry , HIV Integrase/metabolism , HIV-1/genetics , Peptides/chemistry , Amino Acid Sequence , Cell Line, Tumor , Cell Survival/drug effects , Cells, Cultured , Circular Dichroism , Drug Delivery Systems , Enzyme-Linked Immunosorbent Assay , HIV-1/chemistry , Humans , Inhibitory Concentration 50 , Models, Molecular , Peptides/genetics , Peptides/pharmacology , Peptidomimetics , Protein BindingABSTRACT
An artificial antigen forming the C34 trimeric structure targeting membrane-fusion mechanism of HIV-1 has been evaluated as an HIV vaccine. The C34 trimeric molecule was previously designed and synthesized using a novel template with C3-symmetric linkers by us. The antiserum produced by immunization of the C34 trimeric form antigen showed 23-fold higher binding affinity for the C34 trimer than for the C34 monomer and showed significant neutralizing activity. The present results suggest effective strategies of the design of HIV vaccines and anti-HIV agents based on the native structure mimic of proteins targeting dynamic supramolecular mechanisms in HIV fusion.
Subject(s)
AIDS Vaccines/chemistry , HIV Antibodies/immunology , HIV Envelope Protein gp41/immunology , Peptide Fragments/immunology , AIDS Vaccines/genetics , Amino Acid Sequence , Animals , Anti-HIV Agents/chemistry , Cell Line , Cells, Cultured , Enzyme-Linked Immunosorbent Assay , HIV Antigens/chemistry , HIV Antigens/immunology , HIV Envelope Protein gp41/chemical synthesis , Humans , Immunization , Male , Mice , Mice, Inbred BALB C , Molecular Sequence Data , Neutralization Tests , Peptide Fragments/chemical synthesisSubject(s)
HIV Envelope Protein gp41/antagonists & inhibitors , HIV Fusion Inhibitors/chemical synthesis , HIV-1/drug effects , Peptide Fragments/chemistry , Peptide Fragments/pharmacology , Amino Acid Sequence , Anti-HIV Agents/pharmacology , Cell Line , HIV Envelope Protein gp41/chemical synthesis , HIV Envelope Protein gp41/chemistry , HIV Envelope Protein gp41/metabolism , HIV Envelope Protein gp41/pharmacology , HIV Fusion Inhibitors/chemistry , HIV Fusion Inhibitors/pharmacology , HIV-1/metabolism , Humans , Peptide Fragments/chemical synthesis , Protein MultimerizationABSTRACT
Structure-activity relationship studies were conducted on HIV integrase (IN) inhibitory peptides which were found by the screening of an overlapping peptide library derived from HIV-1 gene products. Since these peptides located in the second helix of Vpr are considered to have an alpha-helical conformation, Glu-Lys pairs were introduced into the i and i+4 positions to increase the helicity of the lead compound possessing an octa-arginyl group. Ala-scan was also performed on the lead compound for the identification of the amino acid residues responsible for the inhibitory activity. The results indicated the importance of an alpha-helical structure for the expression of inhibitory activity, and presented a binding model of integrase and the lead compound.
Subject(s)
HIV Integrase Inhibitors/chemistry , Peptides/chemistry , vpr Gene Products, Human Immunodeficiency Virus/chemistry , Amino Acid Sequence , Cell Line , Circular Dichroism , Glutamic Acid/chemistry , HIV Integrase Inhibitors/pharmacology , Humans , Lysine/chemistry , Molecular Sequence Data , Peptides/pharmacology , Structure-Activity RelationshipABSTRACT
Anti-HIV peptides with inhibitory activity against HIV-1 integrase (IN) have been found in overlapping peptide libraries derived from HIV-1 gene products. In a strand transfer assay using IN, inhibitory active peptides with certain sequential motifs related to Vpr- and Env-derived peptides were found. The addition of an octa-arginyl group to the inhibitory peptides caused a remarkable inhibition of the strand transfer and 3'-end-processing reactions catalyzed by IN and significant inhibition against HIV replication.