[Role of NO-synthase in stimulation of opiate receptors and kidney oxidative stress resistance]. / Rol' NO-sintazy v stimuliatsii opiatnykh retseptorov i ustoichivosti pochek k oksidativnomu stressu.

It was established that dalarginum injection before ARI (acute renal insufficiency) formation prevented an increases of proteolysis, decrease of SOD (superoxide dismutase), increase of NO2-/NO3- content in kidney tissue. Antioxidant effect of opiate receptor agonist was completely abolished by preliminary injection of OR antagonist--naloxone. Aminoguanidine nitrate (inducible NO-synthase inhibitor) injection removed positive effect of OR stimulation too. Thus OR stimulation increases kidney oxidative stress resistance due to NO-synthase and SOD activation.

[Effect of dalarginum on the level of nitric oxide stable metabolites in rat kidneys during apoptosis activation]. / Vliianie dalargina na soderzhanie metabolitov oksida azota v pochkakh krys pri aktivatsii apoptoza.

The influence of dalarginum on the level of nitric oxide stable metabolites in the rat kidneys was investigated at various models of apoptosis activation (formed by two methods of acute renal failure). Dalarginum (synthetic leu-enkephaline) was entered in a dose of 100 micrograms/kg a day. Detection of apoptosis was carried out by determination of DNA fragmentation. Administration of dalarginum resulted in the significant decrease of NO--nitrite- and nitrate anions stable metabolites content, the latter is capable to be one of the mechanisms of the preparation antiapoptotic effect.

[Apoptosis and pathomorphology of renal tumors]. / Apoptoz i patomorfoz opukholei pochek.

An analysis was performed of renal tumours in 60 patients with kidney cancer and in two patients with oncocytoma. A study was made against morphological investigations on tumour tissue apoptosis and proliferation. The degree of DNA fragmentation in a biopsy specimen proved to be of value as a guide to detection of apoptosis; the activity of proliferation was studied by the MTT method using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide. Apoptosis has been ascertained in 59 out of 62 cases. The degree of apoptosis (the percentage of DNA fragmentation) was between 0 to 56.2%, the apoptotic cell count tending to decrease with the stage of the tumour process. The extent of cell proliferation was 0.8 to 22.6%; in those patients in the T1N0M0 stage it was much lower than in patients in the T2N0M0/T4N x M1. There was no significant difference between the level of apoptosis, proliferation and spreading of metastases. With growing tumour and degree of its differentiation apoptosis comes to be diminished but the activity of proliferation is noted to be on the increase, which event favours spread of the tumour beyond the organ.

[NMR-relaxation of protons in tissue water in evaluating the potential milk-producing ability of cattle]. / IaMR-relaksatsiia protonov tkanevoi vody v otsenke potentsial'noi molochnoi produktivnosti krupnogo rogatogo skota.

Researching tests on defining the production of the agricultural animals is an actual problem of agricultural biochemistry. In this report, for the first time it is proposed to use the method Nuclear Magnetic Resonance to study the bio-chemical mechanisms of the development of the productivity of the dairy cows. The age-dynamism of NMR-characteristics of the blood and the serum of animals of different genetic types has been examined. Further analysis has been executed on the hormonal regulation of the exchange processes in the organism of the animals. A stable correlation has been established between the time of relaxation of the water-tissue protons and the milk-productivity of the cows. The research has been realised to study the influence of the crossbreeding of ukrainian local races with the Holstein-race on the development of hybrid animals.

[Content of components of the renin-angiotensin-aldosterone system of blood plasma of healthy children in the early neonatal period]. / Soderzhanie komponentov renin-angiotenzin-al'dosteronovoi sistemy v plazme krovi zdorovykh detei v rannem neonatal'nom periode.

The dynamics of the renin and angiotensin converting ferment activity and of the aldosterone concentration in the plasma of blood by healthy newborn full-term in the first week of the life after the birth was determined. The normative values of the studied indexes were obtained. The researches have shown the considerable functional tension of the renin-angiotensin-aldosterone system, that has a very important signification for optimal course of adaptation and anabolic processes by the children at the very beginning of the postnatal development.

[Biosynthesis of prostaglandins E2 and F2a by lung tissue in hypoxia and hyperthermia]. / Biosintez prostaglandinov E2 and F2a v tkaniiakh legkikh v usloviiakh gipoksii i gipertermii.

Effect of a hypoxia and hyperthermia on endogenous biosynthesis of prostaglandins E2 and F2 alpha by tissues of lungs was studied. The effect of high temperature (43 degrees C-45 degrees C during I hour) and tissue hypoxemia by, exposition in an atmosphere with carbon monoxide, stimulated rise of formation of derivative of prostaglandins in the right and left lung: PGF2 alpha in 1.2-2.6, and PG E2-in 1.48-2.7 times accordingly. The parity between prostaglandins after experiment remained same, as well as in control group. The increase of a level of biosynthesis of investigated prostaglandins had more expressed character in a right lung, than in left. The reasons, leading to such significant growth of amounts of prostaglandins can be a few. One of them--it, apparently, growth of an amount of substrates for a phospholipase A2 and cyclooxygenase, as to for want of given temperature of a body is sharply increased a state of fluidity of membranes, leading to violation stability of membrane of phospholipids and further their destruction. Second--possible increase of activity of enzymes, leading to derivation of eicosanoids-cyclooxygenase and (or) of the phospholipase A2, leading to mobilization of an arachidonic acid from phospholipids of membranes. In a tissue of lungs after the hyperthermia and hypoxemia the decrease of amount of c-AMP on a background of c-GMP amount growth in the right and in the left lung was observed. For want of than, described above legitimacy was saved, in which the amount c-AMP in a right lung prevails over left. During realization of experiment interesting legitimacy-process, leading to growth the contents PG E2 was detected, also conducted to decrease of an amount c-AMP. The reduction of an amount c-AMP is possible, apparently, to explain the cell destruction, as heating in these parameters stimulates the cell apoptosis development, which conducts to reduction of an amount c-AMP inside a cell.

[Change in prostanoid metabolism in cystic kidney lesion]. / Izmenenie metabolizma prostanoidov pri kistoznom porazhenii pochek.

A wide range of renal prostaglandins in cysts has been studied as compared with their content in the daily urine, activity of plasma renin, level of adrenocorticotropic hormone (ACTH) in the tissues, methionine enkephalin and aldosterone. High activity of renin, met-enkephalin and ACTH was observed in the patients with one cyst. Under renal polycystosis the activity of plasma renin is higher and the amount of ACTH positively decreases. The comparison of prostanoids content in the cystic fluid of a single cyst and polycystic one demonstrates that the polycystic level of all prostanoids in the punctate increase 2-3 times.

[Action of biologically active peptides and steroids on nuclear magnetic resonance relaxation of protons of kidney tissue water in vitro]. / Deistvie biologicheski aktivnykh peptidov i steroidov na iaderno-magnitno-rezonansnuiu relaksatsiiu protonov tkanevoi vody pochek in vitro.

The effect of biologically active peptides (adrenocorticotropin, parathyroid hormone, calcitonin, prolactin) and steroids (aldosterone, progesterone, testosterone) hormones on NMR-relaxation proton of tissue water of kidney have been investigated in vitro. Results of this study suggest that peptide hormones caused dehydration of tissues via sodium regulation. Steroids, quite on the contrary, caused the hydration of kidney tissue independent of the movement of osmotic active electrolyte.

[Age and features of prostanoid biosynthesis in the rat kidney]. / Vozrastnye osobennosti biosinteza prostoidov v pochkakh krys.

The biosynthesis of PgF2, 6-keto-PgF1 alpha and TxB2 from endogenous precursor in vitro by the rat kidney cortex and medulla in ontogenesis was found to be highest at young age reducing from papillae to kidney cortex. The main product of the biosynthesis in kidney medulla of young and adult rats was PgE.