[The use of distal occlusion correctors during treatment on an individual lingual bracket system in adult patients]. / Primenenie korrektorov distal'noi okklyuzii pri lechenii s ispol'zovaniem individual'noi lingval'noi breket-sistemy u vzroslykh patsientov.

A case of treatment of a 35-year-old patient with a retroposition of the lower jaw, distal occlusion of the posterior teeth, deep incisal overlap, narrowing of the deformity of the dentoalveolar arches, protrusion of the lower incisors, crowded position of the teeth, mismatch between the incisor lines, absence due to extraction of tooth 3.6, with impacted dystopic tooth 3.8 is presented. Fixed individual orthodontic appliances of the WIN («DW Lingual Systems GmbH¼ Germany) were used. It was found that the use of the Herbst apparatus in combination with the WIN lingual bracket system in adult patients helps to achieve optimal functional and aesthetic results. The use of a distal bite correction device is an additional support for the mesialization of the molars of the lower jaw.

[Elimination of discoordination of the masticatory muscles work in patients with muscular-articular dysfunction of the temporomandibular joint by using «elastocorrector¼ appliance]. / Primenenie elastokorrektora dlya ustraneniya diskoordinatsii raboty zhevatel'nykh myshts u patsientov s disfunktsiei visochno-nizhnechelyustnogo sustava.

The aim of the study was to analyze the effectiveness of elastocorrective therapy in the complex treatment of patients with musculo-articular dysfunction of TMJ. Patients (n=237) underwent a comprehensive examination, including: 1) analysis of control and diagnostic plaster models of the jaws; 2) clinical examination; 3) X-ray examination: panoramic X-ray and TRG in lateral projection, computed tomography (CT) of the temporomandibular joint or X-ray zonography of the temporomandibular joint, and magnetic resonance tomography (MRI) of the temporomandibular joint, 4) electromyography of the chewing muscles, 5) T-Scan occlusion computer analysis; 6) stabilometry. After clinical, X-ray examination and data analysis of functional research methods, an algorithm for diagnostic and therapeutic measures was developed. All patients were prescribed the use of elastocorrector: at night during sleep and during the day for 1.5-2 hours. As a result of the use of elastocorrector in patients with muscle-articular dysfunction of the TMJ the position of the mandible was normalized and the spatial ratio of TMJ elements was optimized, the masticatory muscles function and bioelectric activity was normalized and the overload of muscles of mastication during functional movements is eliminated. «Elastocorrector¼ appliance can be used in patients with TMJ musculo-articular dysfunction both for orthodontic treatment (to eliminate chewing muscle hypertonus, compression of intra-articular structures, premature occlusive contacts) and to prepare for further treatment (orthopedic, therapeutic, etc.).

[EMG functional changes in masticatory muscles by elastopositioner use in patients with TMJ dysfunction].

The analysis of the results of EMG studies in patients with TMJ dysfunction was carried out before and after use of elastpositioner "Corrector". The study revealed significant functional disturbances of the masticatory muscles, which were corrected after applying elastpositioner: there was a trend to decreased activity of masseter and temporal muscles, especially in the stagе of rest.

Long-distance signal transmission and regulation of photosynthesis in characean cells.

Photosynthetic electron transport in an intact cell is finely regulated by the structural flexibility of thylakoid membranes, existence of alternative electron-transport pathways, generation of electrochemical proton gradient, and continuous exchange of ions and metabolites between cell organelles and the cytoplasm. Long-distance interactions underlying reversible transitions of photosynthetic activity between uniform and spatially heterogeneous distributions are of particular interest. Microfluorometric studies of characean cells with the use of saturating light pulses and in combination with electrode micromethods revealed three mechanisms of distant regulation ensuring functional coordination of cell domains and signal transmission over long distances. These include: (1) circulation of electric currents between functionally distinct cell domains, (2) propagation of action potential along the cell length, and (3) continuous cyclical cytoplasmic streaming. This review considers how photosynthetic activity depends on membrane transport of protons and cytoplasmic pH, on ion fluxes associated with the electrical excitation of the plasmalemma, and on the transmission of photoinduced signals with streaming cytoplasm. Because of signal transmission with cytoplasmic flow, dynamic changes in photosynthetic activity can develop far from the point of photostimulus application and with a long delay (up to 100 s) after a light pulse stimulus is extinguished.

[Use of elastopositioner for treatment of masticator muscles discoordination in patients with TMJ dysfunction].

The paper presents the analysis of functional changes in patients with TMJ-dysfunction before and after the use of elastopositioner. Analysis has shown that at the initial stages the device provide relief from pain and masticatory muscles hypertension, reduced the bioelectric activity of the muscles. Normalization of the lower jaw movements when opening and closing the mouth and reduction of noise events in TMJ were also noted.

[An Escherichia coli strain producing a leaderless mRNA from the chromosomal lac promoter].

A special Escherichia coli strain capable of producing a leaderless lacZ mRNA from the chromosomal lac promoter was constructed to study the mechanism of leaderless mRNA translation. The translation efficiency of this noncanonical mRNA is very low in comparison with the canonical cellular templates, but it increases by one order of magnitude in the presence of chromosomal mutations in the genes encoding the ribosomal S1 and S2 proteins. The new strain possesses obvious advantages over the commonly used plasmid constructs (first of all, due to the constant dosage of lacZ gene in the cell) and opens possibilities for investigation of the specific conditions for leaderless mRNA translation in vivo using molecular genetic approaches.

[Semidominant effect of the l(1)ts403 (sbr10) mutation on nondisjunction of sex chromosomes in meiosis in Drosophila melanogaster females exposed to heat]. / Poludominantnoe vliianie mutatsii l(1)ts403 (sbr10) na neraskhozhdenie polovykh khromosom v meioze u samok Drosophila melanogaster pri teplovom vozdeistvii.

The sbr gene of Drosophila melanogaster belongs to the NXF (nuclear export factor) family responsible for the mRNA transport from nucleus to cytoplasm. We have shown that in the heat-exposed (37 degrees C, 1 h) females, the l(1)ts403 (sbr10) mutation leads, in particular, to the high-frequency nondisjunction and loss of sex chromosomes in meiosis. For this trait, the incomplete dominance of the sbr10 mutation is observed. At the same time, the sbr10 mutation is recessive for many other traits of the heat-exposed flies: reduced viability, low fertility, impaired synthesis of the heat shock proteins, etc. The females heterozygous for the null allele (Df(1)vL4, a deletion eliminating gene srb) do not differ from females homozygous for the wild-type allele in frequency of the heat shock-induced nondisjunction and loss of sex chromosomes in meiosis. Because of this, the sbr10 mutation can be assigned to the gain-of-function alleles (those gaining the dominance function). Expression of the mutant sbr10 allele against the background of the wild-type allele suggests that in the heat shock-exposed females, the heat-modified product of this ts allele has a strong effect on sex chromosome disjunction in meiosis.

[Extensive complementarity of the Shine-Dalgarno region and 3'-terminal sequence of 16S ribosomal RNA is inefficient for translation in vivo]. / Izbytochnaia komplementarnost' oblasti Shaina-Dal'garno i 3'-kontsevogo uchastka 16S ribosomnoi RNK neéffektivna pri transliatsii in vivo.

Translation initiation in Escherichia coli involves as a rule complementary interactions between a Shine-Dalgarno (SD) sequence upstream of the initiation codon and a highly conserved 3'-end sequence of 16S rRNA (anti-SD). The translation efficiency is believed to be directly affected by the affinity of the ribosome to the mRNA initiation region. Earlier, high-affinity RNA ligands to E. coli ribosomes were selected by the SELEX approach, with the ligands containing an extended SD-sequence well represented. In this work, we examined the ability of artificial ribosome binding sites (RBSs) containing such an extended (10-nt) SD-sequence (superSD) to drive translation in vivo, as well as its ability to form the translation initiation complex in vitro. Toe print experiments showed the formation of a ternary initiation complex on mRNA comprising superSD. Moreover, they proved the formation of an extended SD-duplex in the binary 30S-mRNA complex. Nevertheless, the superSD appeared to be inefficient in translation in vivo. We believe that the initiation complex involving a superSD-element is too stable to be functional; it may impede the transition from initiation to elongation, thus disrupting the transcription-translation coupling and inhibiting the formation of polysomes.

[Development of thermotolerance in Drosophila melanogaster line l(1) ts403 with a defect in heat shock protein synthesis]. / Formirovanie termotolerantnosti u linii Drosophila melanogaster l(1) ts403 s narushennym sintezom belkov teplovogo shoka.

To elucidate the role of heat shock proteins (HSP) in the formation of resistance to extreme factors and in the development of organismic and cell response to these factors, thermotolerance in a Drosophila melanogaster line with defective HSP synthesis was studied with regard to several criteria: (1) survival of adult-females; (2) damage to egg chambers in ovarioles; (3) dynamics of oviposition; (4) frequency of loss and nondisjunction of sex chromosomes in meiosis of females exposed to a heat shock (HS). According to all these criteria, the l(l)ts403 females were more sensitive to a HS 37 degrees C, the exposure at 37 degrees C for 1 h (HS37) than the females of the wild-type line Canton S. Only the data on the first three aforementioned parameters were indicative of thermotolerance development. In files exposed to HS35 followed by HS37, a decrease in nondisjunction and loss of sex chromosomes in the 3- and 4-day ovipositions was observed as compared to the flies exposed to HS37 only. This can be explained by the differences in oviposition dynamics and, consequently, in the realization rate of the oogenesis stages in these two experimental variants. The pleiotropic effect of the l(l)ts403 mutation that led to a disturbance in HSP gene expression at the posttranscriptional level and to an increase in the frequency of sex chromosome nondisjunction in the meiosis of females exposed to HS37 suggests that these processes were connected. As no thermotolerance was revealed by studying the sex chromosome nondisjunction, but thermotolerance was found by estimating the other parameters, we suggest that the product of the gene studied is involved in a signal system operating at a stage that precedes the HS-induced changes in the translation and division apparatuses of a cell.