Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 2 de 2
Filter
Add more filters










Database
Language
Publication year range
1.
Transplant Proc ; 52(7): 2198-2203, 2020 Sep.
Article in English | MEDLINE | ID: mdl-32690311

ABSTRACT

Amniotic stem cells promote adhesion and migration of epithelial cells. Obtaining a full sheet containing amniotic stem cells seems to be the best solution for the treatment of burn wounds. The main advantage of this method is obtaining a full sheet of cells by lowering the temperature below the transition temperature, which does not affect extracellular matrix. The purpose of this work was to produce a skin substitute-a full sheet consisting of amniotic mesenchymal stem cells-and compare with well-known fibroblast sheet. Amniotic membrane cells revealed better tendency to full sheet detachment than fibroblasts. Confluence after 24 hours was always higher on polymer-coated dishes than on normal polypropylene dishes. Also viability was higher than on the control culture dish, while the number of apoptotic cells was always highest on polypropylene (control). Ile-Lys-Val-ala-Val (IKVAV) 0.28 addition to poly (poly [ethylene glycol] ethyl methacrylate) (PTEGMA) caused best cell confluence and highest percentage of cells in mitosis phase of cell cycle, but also worst cell detachment was observed in both cell types on PTEGMA IKVAV 0.28. Viability of cells transferred in cell sheet form onto a new culture dish was higher than when detached as suspension enzymatically. Additionally, percentage of apoptotic cells transferred in cell sheet form onto a new culture dish was always lower than when detached as suspension enzymatically. Culturing of PTEGMA, PTEGMA IKVAV 0.28 and PTEGMA IKVAV 0.14 have a stimulating effect on number of cells in mitosis in amniotic cell culture even after cell sheet transfer onto a new dish, whereas such effect with fibroblast was not observed.


Subject(s)
Cell Culture Techniques/methods , Mesenchymal Stem Cells/cytology , Methacrylates , Polyethylene Glycols , Tissue Engineering/methods , Amnion/cytology , Animals , Cell Adhesion , Cells, Cultured , Female , Pregnancy , Temperature
2.
Carbohydr Polym ; 91(1): 115-27, 2013 Jan 02.
Article in English | MEDLINE | ID: mdl-23044112

ABSTRACT

In this study, we present facile antimicrobial finishing of cotton textiles. Screen printing was used for surface-finishing of cotton using a printing paste containing silver nitrate. UVC irradiation was applied to convert silver nitrate into a color product, thus also changing the color of the textiles. The color, its strength and stability of samples, depend on absorbed UVC energy and the formula of the printing paste. Scanning electron microscopy with the energy dispersive X-ray spectrometry revealed formation of silver particles on cotton threads; X-ray diffraction analysis and the time-of-flight secondary ion mass spectrometry did not provide clear information on these products. Microbiological studies revealed that the samples inhibited proliferation of Escherichia coli, Bacillus subtilis and Staphylococcus aureus. Washing fastness tests confirmed resistance of the samples to at least 50 washings. Additionally, the inhibition zones increased as the number of washing cycles increased, which is unique for such samples. This work also presents an approach to the design of antimicrobially finished workwear.


Subject(s)
Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/pharmacology , Cotton Fiber , Silver Nitrate/chemistry , Ultraviolet Rays , Bacteria/drug effects , Drug Stability , Protective Clothing/microbiology , Tensile Strength , Time Factors
SELECTION OF CITATIONS
SEARCH DETAIL
...