ABSTRACT
The symbiotic nitrogen-fixing soil bacterium Sinorhizobium meliloti contains three replicons: pSymA, pSymB, and the chromosome. We report here the complete 1,354,226-nt sequence of pSymA. In addition to a large fraction of the genes known to be specifically involved in symbiosis, pSymA contains genes likely to be involved in nitrogen and carbon metabolism, transport, stress, and resistance responses, and other functions that give S. meliloti an advantage in its specialized niche.
Subject(s)
Plasmids/genetics , Sinorhizobium meliloti/genetics , Agrobacterium tumefaciens/genetics , Amino Acids/metabolism , Calcium-Binding Proteins/genetics , Carrier Proteins/genetics , DNA, Bacterial/genetics , Eukaryotic Cells/metabolism , Gene Expression Regulation, Bacterial/genetics , Gene Library , Genes, Bacterial , Molecular Sequence Data , Nitrogen/metabolism , Nitrogen Fixation/genetics , Phenotype , Replicon/genetics , Sequence Analysis, DNA , Species Specificity , Transcription, Genetic/geneticsABSTRACT
The scarcity of usable nitrogen frequently limits plant growth. A tight metabolic association with rhizobial bacteria allows legumes to obtain nitrogen compounds by bacterial reduction of dinitrogen (N2) to ammonium (NH4+). We present here the annotated DNA sequence of the alpha-proteobacterium Sinorhizobium meliloti, the symbiont of alfalfa. The tripartite 6.7-megabase (Mb) genome comprises a 3.65-Mb chromosome, and 1.35-Mb pSymA and 1.68-Mb pSymB megaplasmids. Genome sequence analysis indicates that all three elements contribute, in varying degrees, to symbiosis and reveals how this genome may have emerged during evolution. The genome sequence will be useful in understanding the dynamics of interkingdom associations and of life in soil environments.
Subject(s)
Genome, Bacterial , Sequence Analysis, DNA , Sinorhizobium meliloti/genetics , Symbiosis/genetics , Bacterial Adhesion , Bacterial Proteins/genetics , Carrier Proteins/genetics , Chromosomes, Bacterial/genetics , Computational Biology , DNA Transposable Elements , Energy Metabolism/genetics , Evolution, Molecular , Gene Duplication , Genes, Bacterial , Genes, Essential , Genes, Regulator , Medicago sativa/microbiology , Nitrogen/metabolism , Nitrogen Fixation/genetics , Plasmids , Polysaccharides, Bacterial/genetics , Replicon , Rhizobiaceae/genetics , Sinorhizobium meliloti/physiologyABSTRACT
Candida albicans is a diploid fungus that has become a medically important opportunistic pathogen in immunocompromised individuals. We have sequenced the C. albicans genome to 10.4-fold coverage and performed a comparative genomic analysis between C. albicans and Saccharomyces cerevisiae with the objective of assessing whether Candida possesses a genetic repertoire that could support a complete sexual cycle. Analyzing over 500 genes important for sexual differentiation in S. cerevisiae, we find many homologues of genes that are implicated in the initiation of meiosis, chromosome recombination, and the formation of synaptonemal complexes. However, others are striking in their absence. C. albicans seems to have homologues of all of the elements of a functional pheromone response pathway involved in mating in S. cerevisiae but lacks many homologues of S. cerevisiae genes for meiosis. Other meiotic gene homologues in organisms ranging from filamentous fungi to Drosophila melanogaster and Caenorhabditis elegans were also found in the C. albicans genome, suggesting potential alternative mechanisms of genetic exchange.
Subject(s)
Candida albicans/genetics , Genome, Fungal , Meiosis/physiology , Animals , Candida albicans/cytology , Candida albicans/metabolism , Candida albicans/physiology , Morphogenesis , Signal Transduction , Spores, FungalABSTRACT
Gene order evolution in two eukaryotes was studied by comparing the Saccharomyces cerevisiae genome sequence to extensive new data from whole-genome shotgun and cosmid sequencing of Candida albicans. Gene order is substantially different between these two yeasts, with only 9% of gene pairs that are adjacent in one species being conserved as adjacent in the other. Inversion of small segments of DNA, less than 10 genes long, has been a major cause of rearrangement, which means that even where a pair of genes has been conserved as adjacent, the transcriptional orientations of the two genes relative to one another are often different. We estimate that about 1,100 single-gene inversions have occurred since the divergence between these species. Other genes that are adjacent in one species are in the same neighborhood in the other, but their precise arrangement has been disrupted, probably by multiple successive multigene inversions. We estimate that gene adjacencies have been broken as frequently by local rearrangements as by chromosomal translocations or long-distance transpositions. A bias toward small inversions has been suggested by other studies on animals and plants and may be general among eukaryotes.
Subject(s)
Candida albicans/genetics , Chromosome Inversion , Evolution, Molecular , Genes, Fungal , Saccharomyces cerevisiae/genetics , Chromosomes, Fungal , Gene Rearrangement , Genome, FungalABSTRACT
Here we report the sequence of 569,202 base pairs of Saccharomyces cerevisiae chromosome V. Analysis of the sequence revealed a centromere, two telomeres and 271 open reading frames (ORFs) plus 13 tRNAs and four small nuclear RNAs. There are two Tyl transposable elements, each of which contains an ORF (included in the count of 271). Of the ORFs, 78 (29%) are new, 81 (30%) have potential homologues in the public databases, and 112 (41%) are previously characterized yeast genes.
