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1.
J Antibiot (Tokyo) ; 65(8): 427-31, 2012 Aug.
Article in English | MEDLINE | ID: mdl-22669446

ABSTRACT

A novel Gram-stain-positive actinobacterium, designated RI148-Li105(T), was isolated from a lichen sample from Rishiri Island, Japan, and its taxonomic position was investigated by a polyphasic approach. 16S rRNA gene sequencing study indicated that strain RI148-Li105(T) was related to the type strain of Luteimicrobium subarcticum, with a similarity of 97.8%. Cells of strain RI148-Li105(T) exhibited a rod-coccus cycle. The diagnostic cell-wall diamino acid of this organism was lysine and the peptidoglycan type was found to be A4α. The predominant menaquinones were MK-8(H(2)) and MK-9(H(2)), and the major fatty acids were iso-C(16:0), C(17:1) ω9c and C(17:0). The DNA G+C content was 73.6 mol%. The major phenotypic characteristics of strain RI148-Li105(T) basically corresponded to those of the genus Luteimicrobium excluding the fatty acid composition. These results suggest that strain RI148-Li105(T) should be affiliated with the genus Luteimicrobium. Meanwhile, DNA-DNA hybridization and some phenotypic characteristics revealed that the strain differs from L. subarcticum. Therefore, strain RI148-Li105(T) represents a novel species of the genus Luteimicrobium, for which the name Luteimicrobium album sp. nov. is proposed. The type strain of Luteimicrobium album is RI148-Li105(T) (=NBRC 106348(T)=DSM 24866(T)).


Subject(s)
Actinobacteria/isolation & purification , Lichens/microbiology , Actinobacteria/chemistry , Actinobacteria/genetics , Actinobacteria/ultrastructure , Amino Acids/analysis , Base Composition , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , Fatty Acids/analysis , Japan , Microscopy, Electron, Scanning , Phylogeny , RNA, Ribosomal, 16S/chemistry , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Vitamin K 2/analysis
2.
Int J Syst Evol Microbiol ; 62(Pt 4): 795-799, 2012 Apr.
Article in English | MEDLINE | ID: mdl-21571941

ABSTRACT

A non-motile and non-endospore-forming rod, strain NBRC 16403T, was isolated from the phyllosphere of a sedge (Carex sp.). 16S rRNA gene sequence analysis indicated that strain NBRC 16403T was closely related to Herbiconiux solani DSM 19813T (98.6 % 16S rRNA gene sequence similarity), Herbiconiux ginsengi wged11T (97.8 %) and Herbiconiux moechotypicola RB-62T (97.8 %). The peptidoglycan (B2γ type) contained D- and l-2,4-diaminobutyric acids, d-alanine, glycine and threo-3-hydroxyglutamic acid, which replaced glutamic acid almost completely. The predominant menaquinones were MK-10 and MK-11. The polar lipid pattern comprised diphosphatidylglycerol, phosphatidylglycerol, three glycolipids and minor amounts of other polar lipids. The major fatty acids were anteiso-C15:0, iso-C16:0 and anteiso-C17:0; no cyclohexyl-C17:0 was detected. The DNA G+C content was 71.0 mol%. The results of phylogenetic and DNA-DNA relatedness studies, along with phenotypic differences between strain NBRC 16403T and recognized members of the genus Herbiconiux, indicated that the isolate should be assigned to a novel species of the genus Herbiconiux, for which the name Herbiconiux flava sp. nov. is proposed. The type strain is NBRC 16403T (=VKM Ac-2058T).


Subject(s)
Actinomycetales/classification , Carex Plant/microbiology , Phylogeny , Soil Microbiology , Actinomycetales/genetics , Actinomycetales/isolation & purification , Bacterial Typing Techniques , Base Composition , DNA, Bacterial/genetics , Fatty Acids/chemistry , Molecular Sequence Data , Nucleic Acid Hybridization , Peptidoglycan/chemistry , RNA, Ribosomal, 16S/genetics , Russia , Sequence Analysis, DNA , Vitamin K 2/chemistry
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