ABSTRACT
A study was made of changes in nucleic acid synthesis in proliferating germ cell populations of animals irradiated with doses of 2 and 4 Gy. The administration of exogenous testosterone was shown to stimulate DNA synthesis, inhibited by the effect of radiation, in spermatogonium populations of A1-4, intermediate, and B types, and in preleptotene primary spermatocytes; RNA synthesis was increased in the same populations of spermatogonia and pachytene primary spermatocytes. The obtained results indicate that exogenous testosterone exerts a beneficial effect on spermatogenesis damaged by ionizing radiation.
Subject(s)
DNA/biosynthesis , RNA/biosynthesis , Radiation Injuries, Experimental/metabolism , Spermatozoa/metabolism , Testosterone/pharmacology , Animals , Cobalt Radioisotopes , Gamma Rays , Male , Mice , Spermatozoa/drug effects , Spermatozoa/radiation effects , Whole-Body IrradiationABSTRACT
Cytological analysis showed that disturbances referable to division and development of spermatogonia (intermediate and B types) and primary spermatocytes in irradiated animals are modified by injection of exogenous testosterone. It was established that the hormone exerts its modifying effect after radiation doses affecting mechanisms of spermatogenesis regulation. Testosterone bound to target cells initiates their division and decreases duration of spermatogonium mitosis block and delay in primary spermatocyte development increasing the number of germ cells.
Subject(s)
Spermatogenesis/radiation effects , Testosterone/pharmacology , Animals , Cobalt Radioisotopes , Gamma Rays , Male , Mice , Spermatogenesis/drug effects , Whole-Body IrradiationSubject(s)
Testis/radiation effects , Animals , Chromosomes/radiation effects , DNA/radiation effects , Dose-Response Relationship, Radiation , Gamma Rays , Guinea Pigs , Leydig Cells/radiation effects , Male , Mitosis/radiation effects , Mutation , Neutrons , RNA/radiation effects , Rabbits , Ribonucleoproteins/radiation effects , Seminiferous Tubules/radiation effects , Sertoli Cells/radiation effects , Spermatogenesis/radiation effects , Spermatogonia/radiation effects , Testosterone/radiation effects , X-RaysSubject(s)
Testis/radiation effects , Animals , Dogs , Gamma Rays , Male , Radiation Dosage , Time FactorsABSTRACT
The Sertoli's and Leidig's cells (sustentocytes and glandulocytes) exposed to ionizing radiation were studied cytologically and histochemically. The experiments were made on white male mice which were irradiated in dosage from 50 to 400 r. The DNP of the nuclei of sustentocytes and glandulocytes were investigated at different periods of the spermatogenesis cycle and under hormonal loading. The irradiation was established to result in changes in the chromatine state of both types of cells, the Sertoli's cells being more resistant to radiation. Injection of testosterone at different periods after irradiation causes a decreased dissociation of DNP. Inspite of the apparent morphological stability to ray effects the sustentocytes and glandulocytes are highly reactive elements.
