ABSTRACT
The chemical and biological characterization of the preparation, found to be strictly specific, is presented. The allergen is nontoxic and has sensitizing properties. The active principle of the preparation is protein.
Subject(s)
Allergens/immunology , Trichomonas/immunology , Allergens/analysis , Allergens/toxicity , Animals , Chemical Phenomena , Chemistry, Physical , Dose-Response Relationship, Immunologic , Guinea Pigs , Immunization , Mice , Skin TestsSubject(s)
Allergens/analysis , Neisseria gonorrhoeae/immunology , Allergens/immunology , Allergens/toxicity , Amino Acids/analysis , Animals , Chemical Phenomena , Chemistry, Physical , Dose-Response Relationship, Immunologic , Gonorrhea/immunology , Guinea Pigs , Humans , Immunization , Mice , Skin Tests , Time FactorsABSTRACT
The fraction composition of allergens obtained by different methods from the microbial mass of N. meningitidis, N. gonorrhoeae and N. perflava has been studied. Each method produced its characteristic number of fractions, irrespective of the Neisseria species used. Their molecular weights: more than 2,000,000 daltons, 160,000 daltons, 31,000 daltons and 14,000 daltons. All these fractions are biologically active.
Subject(s)
Allergens/isolation & purification , Chromatography, Gel/methods , Neisseria/immunology , Chemical Fractionation/methods , Molecular Weight , Neisseria gonorrhoeae/immunology , Neisseria meningitidis/immunology , Sepharose/pharmacologyABSTRACT
The sensitizing activity of meningococcal cells and their fractions has been studied. Only protein-containing preparations have been shown to be capable of inducing delayed hypersensitivity, while polysaccharide fractions do not induce it.
Subject(s)
Hypersensitivity, Delayed/immunology , Neisseria meningitidis/immunology , Animals , Bacterial Proteins/immunology , Chemical Phenomena , Chemistry , Guinea Pigs , Polysaccharides, Bacterial/immunology , Salmonella typhi/immunology , Skin TestsABSTRACT
Various methods of isolating allergen fractions from N. meningitidis, N. gonorrhoeae and N. perflava were tested. The biological activity of the preparation was found to depend on the method of its production, which determined its chemical composition. When gonococcal and meningococcal allergens and N. perflava allergen were used in skin tests, cross reactions were observed. Nevertheless, as the intensity and size of skin reaction was much greater when a homologous preparation was administered, it was possible to differentiate the presence of sensitization to a definite microbial species. Electrophoresis in acrylamide gel revealed the heterogeneity of allergen preparations. The ability of the preparation to induce skin reaction was not connected with its serological properties.
