ABSTRACT
The initiation of translation in eukaryotic cells is stimulated by proteins known as initiation factors (eIFs). A structurally complex eIF composed of multiple subunits, eIF3 has been shown to have various functions in translation in a variety of eukaryotes. Until now, little is known about eIF3 in Eimeria tenella. Based on a previously identified expressed sequence tag(EST), we cloned the eIF3 subunit 7 gene (EteIF3s7) from E. tenella by rapid amplification of the cDNA ends(RACE). The 2278-bp full-length complementary DNA of EteIF3s7 contained a 1716-bp open reading frame (ORF) that encoded a 571-amino acid (aa) polypeptide. The EteIF3s7 protein contained the subunit 7 domain that is characteristic of members of the eIF3 zeta superfamily. The levels of EteIF3s7 messenger RNA and protein were higher in second generation merozoites than in sporulated oocysts, unsporulated oocysts, or sporozoites, and the EteIF3s7 protein was barely detectable in unsporulated oocysts. Our immunofluorescence analysis showed that the EteIF3s7 protein was uniformly distributed throughout the cytoplasm of sporozoites. After sporozoites were incubated in complete medium, the EteIF3s7 protein localized to the anterior region of the parasite. Following the first schizogenous division, the protein was uniformly dispersed in trophozoites, immature schizonts, and mature schizonts, and the EteIF3s7 protein was observed to be closely associated with the parasitophorous vacuole membrane. An anti-rEteIF3s7 polyclonal antibody inhibited the ability of E. tenella to invade DF-1 cells, which suggested that EteIF3s7 might be involved in host cell invasion and required for the growth of the parasite in the host.
Subject(s)
Eimeria tenella/chemistry , Eukaryotic Initiation Factor-3/physiology , Amino Acid Sequence , Animals , Antibodies, Protozoan/immunology , Base Sequence , Cell Line , Chick Embryo , Chickens , Cloning, Molecular , DNA, Complementary/chemistry , DNA, Complementary/genetics , Dose-Response Relationship, Immunologic , Eimeria tenella/genetics , Eimeria tenella/immunology , Eukaryotic Initiation Factor-3/chemistry , Eukaryotic Initiation Factor-3/genetics , Eukaryotic Initiation Factor-3/immunology , Fibroblasts/parasitology , Molecular Sequence Data , RNA, Messenger/analysis , RNA, Messenger/isolation & purification , RNA, Protozoan/analysis , RNA, Protozoan/isolation & purification , Rabbits , Specific Pathogen-Free OrganismsABSTRACT
The prevalence of coccidial infections in dairy cattle was examined in Shanghai from November 2010 to March 2011. In total, 626 fecal samples from 24 dairy farms were examined; oocysts were identified to the species level based on morphological features. All herds were infected with Eimeria species. The overall prevalence of coccidia was 47.1%, with the highest prevalence in <4-mo-old calves (51.8%) and the lowest in >12-mo-old cattle (27.0%). The number of oocysts per gram of feces was significantly higher in young calves than in weaners and adults. Ten species of Eimeria were identified, among which Eimeria ellipsoidalis, Eimeria bovis, Eimeria zuernii, and Eimeria alabamensis were the predominant species. Concurrent infection with 2-8 species was common.
Subject(s)
Cattle Diseases/epidemiology , Coccidiosis/veterinary , Eimeria/isolation & purification , Age Factors , Animals , Cattle , Cattle Diseases/parasitology , China/epidemiology , Coccidiosis/epidemiology , Eimeria/classification , Feces/parasitology , Oocysts/classification , Parasite Egg Count/veterinary , PrevalenceABSTRACT
Few data are available on the prevalence of Eimeria spp. in yaks. An observational study was conducted to determine the prevalence of coccidial infection in yaks on the Qinghai-Tibet Plateau of China. A total of 324 fecal samples from 4 counties was examined, and oocysts were identified to the species level on the basis of morphological features. Eimeria oocysts were found in 113 (34.9%) samples. The species detected and their prevalence values included the following: Eimeria zuernii (54.9%), E. pellita (35.4%), E. canadensis (33.6%), E. bovis (23.0%), E. cylindrica (16.8%), E. subspherica (16.8%), E. ellipsoidalis (14.1%), E. brasiliensis (13.3%), E. wyomingensis (8.0%), E. alabamensis (7.1%), E. illinoisensis (5.3%), E. auburnensis (4.4%), E. bombayansis (3.5%), and E. bukidnonensis (2.7%). Mixed infections of 2 to 7 species were found in 66.4% of the animals. There was an age-related difference in the prevalence of infection. The highest prevalence (53.3%) was observed in calves, an intermediate prevalence in yearlings (36.1%), and the lowest was in adults (15.6%). The number of oocysts per g of feces was significantly higher in calves than in adults. More Eimeria species were indentified in calves. Eimeria zuernii was the most prevalent species in calves and adults, whereas in yearling yaks E. pellita was most common. The majority of calves and yearlings showed mixed infection, but adults tended to be infected with 1 species. The prevalence and intensity of Eimeria species were found to show statistically significant differences among different regions in Qinghai Province.
