ABSTRACT
OBJECTIVE: The purpose of this study was to explore the expression and function of long non-coding RNA (lncRNA) PCAT6 in ovarian cancer. PATIENTS AND METHODS: Quantitative Real-Time-Polymerase Chain Reaction (qRT-PCR) was used to detect the expression of lncRNA PCAT6 in 42 pairs of ovarian cancer tissues and adjacent normal tissues. Then, the relationship between PCAT6 expression and pathological indicators of ovarian cancer was analyzed. Subsequently, the transfection efficiency of PCAT6 in ovarian cancer cells was verified, and the PCAT6 knockdown model was constructed using lentiviruses in SKOV3 and CAOV3 ovarian cancer cell lines. In addition, Cell Counting Kit-8 (CCK-8) test, wound healing assay and transwell invasion and migration experiments were performed to estimate the effect of PCAT6 on the biological function of ovarian cancer cells, to further explore the possible potential mechanisms. RESULTS: QRT-PCR results showed that the expression level of PCAT6 in ovarian cancer was higher than that in the adjacent normal tissues. The incidence of distant metastasis and lymph node metastasis in patients with high expression of PCAT6 was higher than those with low PCAT6 expression. Compared with the NC group, the proliferation, metastasis and invasion ability of ovarian cancer cells in si-PCAT6 group decreased significantly. QRT-PCR results demonstrated that the PTEN expression was increased after the knockdown of PCAT6. In addition, the recovery experiment also revealed that PCAT6 and PTEN have a mutual regulation, which can jointly regulate the development of ovarian cancer. CONCLUSIONS: LncRNA PCAT6 was up-regulated in ovarian cancer tissues and was closely related to distant metastasis or lymph node metastasis. Additionally, lncRNA PCAT6 might promote the proliferation, migration and invasion of ovarian tumor cells by inhibiting PTEN.
Subject(s)
Ovarian Neoplasms/metabolism , PTEN Phosphohydrolase/metabolism , RNA, Long Noncoding/metabolism , Blotting, Western , Cell Line, Tumor , Cell Movement , Cells, Cultured , Female , Gene Expression Regulation, Neoplastic , Humans , Lymphatic Metastasis , Neoplasm Invasiveness , Ovarian Neoplasms/etiology , Ovarian Neoplasms/pathology , Ovary/metabolism , Real-Time Polymerase Chain ReactionABSTRACT
The epidemiology of 54 episodes of candiduria with respect to clinical risk factors, species of Candida and physician response to the isolation of Candida in urine were studied in an observational survey over 3 months. Candida spp. were isolated from 4.7% of positive urine cultures. Common predisposing conditions included antibiotic use (74.1%), urinary drainage devices (57.4%), surgery (51.9%), intensive care unit (ICU) or high-dependency care unit (HDU) admission (42.6%) and urinary tract (UT) disease (18.5%). Upper UT infection was uncommon (n = 3). Of 65 Candida isolates, C. albicans predominated (85.2%), followed by C. glabrata (27.8%) and other Candida spp. (6.2%). All isolates were susceptible to fluconazole, itraconazole, voriconazole, amphotericin and caspofungin. Indwelling urinary catheters were removed in 76.2% of episodes. Antifungal therapy was initiated in 33.3% of cases independently of patient symptoms, underlying disease or Candida colony count. Patients in ICU/HDUs were significantly more likely to receive antifungal agents than those outside these units (p < 0.001). Fluconazole was the most common drug prescribed (77.8%). Clearance of candiduria occurred independently of antifungal therapy (p = 0.60). Physicians often did not follow up a positive urine result for Candida. Efforts to increase clinician awareness of current recommendations for managing candiduria and further study to elucidate specific risk factors in defined patient populations are warranted.
Subject(s)
Candidiasis/epidemiology , Cross Infection/epidemiology , Health Services Research , Urinary Tract Infections/epidemiology , Urine/microbiology , Adolescent , Adult , Aged , Aged, 80 and over , Antifungal Agents/pharmacology , Antifungal Agents/therapeutic use , Candida/classification , Candida/isolation & purification , Candidiasis/drug therapy , Catheters, Indwelling/adverse effects , Child , Child, Preschool , Critical Care , Female , Hospitalization , Humans , Infant , Infant, Newborn , Male , Microbial Sensitivity Tests , Middle Aged , Urinary Tract Infections/drug therapyABSTRACT
The study involved 355 specimens of STD clinic patients collected from Beijing, Shantou and Wuhan, for the detection of Neisseria gonorrhoeae, Chlamydia trachomatis, Ureaplasma urealyticum by polymerase chain reaction. The results showed that the positive rate of Neisseria gonorrhoeae being the highest in the patients attending STD clinics from the three cities. The detection rate of Neisseria gonorrhoeae in male patients was higher than in females. The positive rates of Chlamydia trachomatis and Ureaplasma urealyticum in the three cities differed from each other greatly. Polymerase chain reaction was suitable for clinical Jetection and epidemiological study of the three kinds of STD pathogens.
