ABSTRACT
Aim: To investigate the anticancer effects of Jinlong capsule (JLC) against human glioblastoma cells and the possible underlying mechanism. Methods: Cell Counting Kit-8 and colony formation assay were adopted for the analysis of cell viability. Cell invasion and migration were evaluated by transwell and wound healing assays. Then, the expression level of mammalian target of rapamycin (mTOR), phosphorylated mTOR (p-mTOR), S6 and phosphorylated S6 (p-S6) were determined by western blotting. Results: The results showed that JLC significantly inhibited human glioblastoma cell proliferation, invasion and migration in a dose-dependent manner. The expressions of p-mTOR and p-S6 were dramatically suppressed by JLC. Furtherly, inhibition of mTOR reduced the cell migration and invasion, while the mTOR agonist (MHY1485) could partially reverse the anti-migration and anti-invasion activity of JLC. Conclusion: The above results suggested that JLC would be a potential candidate for the treatment of glioblastoma.
Subject(s)
Antineoplastic Agents/pharmacology , Cell Movement/drug effects , Drugs, Chinese Herbal/pharmacology , Glioblastoma/drug therapy , Neoplasm Invasiveness , Protein Kinase Inhibitors/pharmacology , Ribosomal Protein S6 Kinases/metabolism , TOR Serine-Threonine Kinases/metabolism , Antineoplastic Agents/chemistry , Capsules/chemistry , Capsules/pharmacology , Cell Proliferation/drug effects , Cell Survival/drug effects , Dose-Response Relationship, Drug , Drug Screening Assays, Antitumor , Drugs, Chinese Herbal/chemistry , Glioblastoma/metabolism , Glioblastoma/pathology , Humans , Molecular Structure , Protein Kinase Inhibitors/chemistry , Signal Transduction/drug effects , Structure-Activity Relationship , Tumor Cells, CulturedABSTRACT
BACKGROUND: We established a glioma biobank at Beijing Tiantan Hospital in November, 2010. Specialized residents have been trained to collect, store and manage the biobank in accordance with standard operating procedures. METHODS: One hundred samples were selected to evaluate the quality of glioma samples stored in the liquid nitrogen tank during different periods (from 2011 to 2015) by morphological examination, RNA integrity determination, DNA integrity determination and housekeeping gene expression determination. RESULTS: The majority of samples (95%) had high RNA quality for further analysis with RIN ≥6. Quality of DNA of all samples were stable without significant degradation. CONCLUSION: Storage conditions of our biobank are suitable for long-term (at least five years) sample preservation with high molecular quality.
