1.
Acta Virol
; 57(3): 333-8, 2013.
Article
in English
| MEDLINE
| ID: mdl-24020758
ABSTRACT
The 2009 swine-origin influenza A virus (H1N1) and its initial reassortant vaccine strains did not grow well in embryonated eggs. The glutamine to arginine mutation at the amino acid position 223 (Q223R) of the hemagglutinin (HA) gene is the major mutation previously found in egg-adapted 2009 H1N1 strains and shown to enhance viral growth in embryonated eggs. However, the effect of this mutation on the receptor-binding preference had not been directly demonstrated. In this study, the Q223R mutation was shown to change the viral HA binding preference from the human-type receptor, α2,6-linked sialic acid, to the avian-type receptor, α2,3-linked sialic acid; and to enhance the viral growth in embryonated eggs but not in cell culture.
Subject(s)
Eggs/virology , Hemagglutinin Glycoproteins, Influenza Virus/genetics , Hemagglutinin Glycoproteins, Influenza Virus/metabolism , Influenza A Virus, H1N1 Subtype/physiology , Influenza in Birds/metabolism , Influenza, Human/metabolism , Mutation, Missense , Receptors, Virus/metabolism , Adaptation, Physiological , Animals , Chick Embryo , Chickens , Hemagglutinin Glycoproteins, Influenza Virus/chemistry , Humans , Influenza A Virus, H1N1 Subtype/chemistry , Influenza A Virus, H1N1 Subtype/genetics , Influenza A Virus, H1N1 Subtype/growth & development , Influenza in Birds/virology , Influenza, Human/virology , N-Acetylneuraminic Acid/chemistry , N-Acetylneuraminic Acid/metabolism , Pandemics , Protein Binding , Receptors, Virus/chemistry
2.
Acta Virol
; 56(3): 253-5, 2012.
Article
in English
| MEDLINE
| ID: mdl-23043606
Subject(s)
Down-Regulation , Influenza A Virus, H1N1 Subtype/physiology , Influenza, Human/genetics , Lung/metabolism , Pulmonary Surfactant-Associated Protein D/genetics , Fatal Outcome , Humans , Influenza A Virus, H1N1 Subtype/isolation & purification , Influenza, Human/metabolism , Influenza, Human/virology , Lung/virology , Pulmonary Surfactant-Associated Protein D/metabolism
3.
Acta Virol
; 56(3): 257-9, 2012.
Article
in English
| MEDLINE
| ID: mdl-23043607