Frequency and type-distribution of human papillomavirus from paraffin-embedded blocks of high grade cervical intraepithelial neoplasia lesions in Thailand.

Cervical cancer is the most important female gynecological cancer, the second leading cause of cancer mortality in women worldwide and the second most common cancer in Thai women. The major cause of cervical cancer is persistent infection of human papillomavirus (HPV), leading to abnormal epithelial lesions, with progression to precancerous and invasive cancer. This study was conducted to investigate the frequency and type distribution of HPV in Thai women who had abnormal cytology. HPV detection from FFPE confirmed abnormal of high grade cervical intraepithelial lesions were for SPF-10-Innogenic Line Probe Assay. HPV-positivity was detected in 320/355 cases (90.14%) and HPV-negativity in 35/355 (9.86%). HPV-positive was found 147/320 cases (41.4%) of single infection, whereas 173/320 cases (48.7%) showed the multiple HPV infection. The most common seven types were HPV-16, -52, -18, -11, -51, -31 and -33, in that order. HPV 16 and 18, the important oncogenic HPV type, were observed in 64.8% of HSIL cases. Interestingly, a high proportion of multiple infections was found in this study and more than ten types could be detected in one case. Therefore, HPV infection screening program in women is essential, particularly in Thailand. Effective primary and secondary prevention campaigns that reinforce HPV screening for HPV detection and typing may be decrease the incidence and mortality of cervical cancer in the future and may lead to significantly improve the quality of life in Thai women.

Prevalence of high-risk human papillomavirus infection and cytologic results in Thailand.

Cervical cancer is the second most common female cancer with a high mortality rate. The established cause is high-risk types of human papillomavirus (HPV) and a new modality for cervical cancer screening is the combination of cervical cytology with HPV testing. The aim of present study was to identify the prevalence of high-risk HPV infection, and cervical cytological profiles of healthy Thai women. This largest cross-sectional investigation of HPV testing so far with cytology screening in Thailand was conducted between April 2009 and March 2010, covering a total of 14,747 women. The correlation between HPV viral load and cytology was also assessed. The mean age of the study group was 46.4 years (range 20-77 years) and the prevalence of high risk HPV infection was 8.23%. In positive women, negative cytology was observed in 72.9% , and cytology abnormalities in 27.1%, as compared to 1.57% in HPV negative women. The highest prevalence of HPV infection was identified in the youngest age group (≤30 years). The mean viral load was 6.06x105 (range 5,040.13 to 1.05x107) and HPV viral load titers were higher among in women with abnormal cytology.