ABSTRACT
The hyperthermophilic archaeon Pyrococcus furiosus can utilize different beta-glucosides, like cellobiose and laminarin. Cellobiose uptake occurs with high affinity (K(m) = 175 nM) and involves an inducible binding protein-dependent transport system. The cellobiose binding protein (CbtA) was purified from P. furiosus membranes to homogeneity as a 70-kDa glycoprotein. CbtA not only binds cellobiose but also cellotriose, cellotetraose, cellopentaose, laminaribiose, laminaritriose, and sophorose. The cbtA gene was cloned and functionally expressed in Escherichia coli. cbtA belongs to a gene cluster that encodes a transporter that belongs to the Opp family of ABC transporters.
Subject(s)
ATP-Binding Cassette Transporters/isolation & purification , ATP-Binding Cassette Transporters/metabolism , Cellobiose/metabolism , Pyrococcus/metabolism , ATP-Binding Cassette Transporters/genetics , Cloning, Molecular , Electrophoresis, Polyacrylamide Gel , Escherichia coli , Kinetics , Molecular WeightABSTRACT
While Euclidean distances and Q factor analysis may sometimes be preferred to correlation coefficients and cluster analysis for developing a typology, commercially available software does not always facilitate their use. Commands are provided for using BMDP and SPSS in a Q factor analysis with Euclidean distances.
