Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 2 de 2
Filter
Add more filters










Database
Language
Publication year range
1.
Low Urin Tract Symptoms ; 11(2): O186-O192, 2019 Apr.
Article in English | MEDLINE | ID: mdl-29266769

ABSTRACT

OBJECTIVE: Stress urinary incontinence (SUI) is a common disease condition in elderly women, suggesting that its etiology may be linked to aging. To investigate the hypothesis that urethral dysfunction and histopathological changes are possible contributors to SUI in elderly women, several parameters of urethral function, as well as histological parameters, were compared between young and aged rats. METHODS: Virgin female rats were examined at 3 different ages, namely 3, 12, and 24 months, corresponding to young, middle-aged, and aged rats, respectively. Urethral function was assessed by measuring the leak point pressure (LPP), pudendal nerve stimulation (PNS)-induced elevation in urethral pressure, and phenylephrine-induced increase in urethral perfusion pressure (UPP). Histopathological assessments were performed following hematoxylin and eosin (HE), Masson's trichrome, and immunofluorescence staining of urethral tissue. RESULTS: LPP of aged rats was significantly reduced compared to that of both young and middle-aged rats. PNS-induced elevation in urethral pressure in aged rats was also significantly lower than that in young rats. In contrast, there were no significant differences in the phenylephrine-induced increase in UPP between young and aged rats. Connective tissue area in the external urethral sphincter (EUS) layer was increased in aged rats, whereas the smooth muscle layer was histologically similar to that in young rats. The number of EUS fibers was significantly reduced in aged rats, whereas the cross-sectional area of EUS fibers increased from differed compared with young rats. CONCLUSION: We have demonstrated age-related changes in EUS function and morphology in the rat urethra, which are considered to be etiological risk factors for SUI in humans.


Subject(s)
Disease Models, Animal , Urethra/physiopathology , Urinary Incontinence, Stress/physiopathology , Aging , Animals , Female , Fluorescent Antibody Technique , Rats , Rats, Sprague-Dawley , Urethra/innervation , Urethra/pathology , Urinary Incontinence, Stress/etiology
2.
J Cereb Blood Flow Metab ; 22(10): 1196-204, 2002 Oct.
Article in English | MEDLINE | ID: mdl-12368658

ABSTRACT

The authors studied the differences between heat-shock/stress protein 70 (hsp70) gene expression and protein synthesis in the unilateral middle cerebral artery (MCA) microsurgical direct occlusion (Tamura's) model and the unilateral intraluminal occlusion model. In Tamura's model, expression of hsp70 mRNA and HSP70 protein and decreased protein synthesis were detected in the ischemic areas, including the ipsilateral cortex and caudate. These phenomena, however, were not observed in the areas outside the MCA territory, including the ipsilateral thalamus, hippocampus, and substantia nigra. These results were consistent among the experimental rats. In the intraluminal occlusion model, however, induction of both hsp70 mRNA and HSP70 protein and impairment of protein synthesis were noted in the areas outside the MCA territory, including the ipsilateral thalamus, hypothalamus, hippocampus, and substantia nigra, as well as in the MCA territory, including the ipsilateral cortex and caudate. These results were not consistent among the experimental rats. These different results might be due to widespread damage resulting from internal carotid artery (ICA) occlusion in the intraluminal occlusion model. Accordingly, the authors suggest that this model be called an ICA occlusion model, rather than a pure MCA occlusion model.


Subject(s)
Blood Coagulation/physiology , Ischemic Attack, Transient/pathology , Middle Cerebral Artery/physiopathology , Animals , Caudate Nucleus/blood supply , Caudate Nucleus/metabolism , Caudate Nucleus/pathology , Cerebral Cortex/blood supply , Cerebral Cortex/metabolism , Cerebral Cortex/pathology , Disease Models, Animal , Gene Expression Regulation , HSP70 Heat-Shock Proteins/genetics , Immunohistochemistry , Ischemic Attack, Transient/genetics , Middle Cerebral Artery/pathology , RNA, Messenger/genetics , Rats , Substantia Nigra/blood supply , Substantia Nigra/metabolism , Substantia Nigra/pathology , Thalamus/blood supply , Thalamus/metabolism , Thalamus/pathology , Time Factors
SELECTION OF CITATIONS
SEARCH DETAIL
...