ABSTRACT
The paper presents the results of laboratory study of the modes of entomopathogenic densonucleasis virus transmission in the population of blood-sucking Culicidae mosquitoes. It was established that densovirus infection was horizontally transmitted through feeding of larvae on the bodies of desonucleasis-infected insects as well as contamination of the environment by infected larvae. Thus, the death rate in populations of various mosquito species attained 25.9-70.8%. Vertical transmission of the virus alters the ratio of sexes at the stage of imago; the survived females and males transmit infection to larvae. Surface sterilization of the ova doesn't prevent larvae infection, death rate among larvae attaining 7.8%.
Subject(s)
Culicidae/microbiology , Insect Viruses , Animals , Female , MaleABSTRACT
Laboratory studies demonstrated the effectiveness of viroden preparation in different doses against preimago stages of Aedes aegypti mosquitoes. After treatment of larvae of instar 1 at preimago stages about 77% of the insects died. The preparation is stable on storage and resistant to unfavourable environmental factors: sun radiation, heating, pH variations, without significant loss of activity. The study of the host range of densonucleosis virus, the active principle of the viroden preparation, showed that it was not harmful for other animals. Large-scale trials of the preparation in different climatic zones are necessary for the elucidation of its effectiveness against naturally occurring mosquito species.