[Content of the antigenic determinants CD16, CD25, CD95, and HLA-DR in induced sputum of patients with bronchial asthma and chronic obstructive bronchitis]. / Soderzhanie antigennykh determinant CD16, CD25, CD95 i HLA-DR v indutsirovannoi mokrote bol'nykh bronkhial'noi astmoi i khronicheskim obstruktivnym bronkhitom.

AIM: To examine the content of CD16, CD25, CD95, and HLA-DR antigenic determinants in induced sputum (IS) of patients with bronchial asthma (BA) and chronic obstructive bronchitis (COB). MATERIALS AND METHODS: The study enrolled 36 patients with BA and 15 with COB whose age was 21 to 58 years. The cellular precipitate of IS served as a material for the study. The studied receptors were determined by immunofluorescence using the "Clonospectrum" monoclonal antibodies. RESULTS: There was an increase in the content of CD16, CD25, HLA-DR, CD95 whose expression was decreased when glucocorticosteroids were used. At remission of asthma, the level of these receptors approximated that in the control group. On an exacerbation of COB, the content of these markers was significantly less than that in the controls and the use of antibacterial agents and methylxanthines led to its rise. At remission of COB, CD16 and CD25 cells were not recorded. CONCLUSION: The levels of membrane markers in IM of patients with BA and COB depend on the period of the disease and used therapy.

[Cytological and biochemical indices of induced sputum in patients with bronchial asthma and chronic obstructive bronchitis]. / Nekotorye tsitologicheskie i biokhimicheskie pokazateli indutsirovannoi mokroty u bol'nykh bronkhial'noi astmoi i chronicheskim obstruktivnym bronkhitom.

The aim of our research was to study the apoptotic index and the representativeness of CD95-receptor in the effector cells, in addition to estimation of the content of tumor necrosis factor-alpha in the induced sputum supernatant observed in patients with bronchial asthma and chronic obstructive bronchitis. These indices were evaluated in the disease dynamics and involves various treatment programs. The object of our research was induced sputum. The analysis has proven that apoptotic index of effector cell nuclei, representativeness of CD95-receptor and the content of tumor necrosis factor-alpha in the induced sputum differed, respectively, in patients with bronchial asthma and chronical obstructive bronchitis, and depended on the period of disease and employed treatment. The induced sputum analysis results can be used for the airway inflammation activity monitoring, with regard to these diseases and to treatment efficiency monitoring.