ABSTRACT
The screening of the positive regulators of landomycin E biosynthesis has been conducted among exogenous metabolites of the museum and freshly isolated Streptomycetes strains by means of cosynthesis test using mutant strain Streptomyces globisporus 1912-82. The ability to produce the exogenous metabolites restoring landomycin E biosynthesis by B2 strain was shown. The regulatory compounds purified by thin-layer chromatography differed between themselves by chromatographic mobility (Rf) and maxima of absorption in UV-light. They were divided into four types of extracellular regulators of landomycin E biosynthesis on the basis of preliminary study.
Subject(s)
Aminoglycosides/biosynthesis , Anti-Bacterial Agents/biosynthesis , Bacterial Proteins/metabolism , Streptomyces/metabolism , Bacterial Proteins/genetics , Bacterial Proteins/isolation & purification , Chromatography, Thin Layer , Mutation , Streptomyces/geneticsABSTRACT
Thirteen antibiotics of red, rose, yellow and violet color, which are synthesized by the soil Streptomycetes on the complete corn-bean medium have been isolated and purified by means of thin layer chromatography. Antibiotic activity of the obtained compounds has been determined against a series of gram-positive and gram-negative microorganisms, and also yeasts. The maxima of absorption of antibiotics in visible and UV-light were established. The preliminary results are obtained about the presence of sugar component in the structure of molecules, as well as the indicator properties of the red-color antibiotics. The assumption was proposed about the affinity of some antibiotics for anthracyclines and ansamycines.
Subject(s)
Anti-Bacterial Agents/isolation & purification , Soil Microbiology , Streptomyces/growth & development , Streptomyces/metabolism , Anti-Bacterial Agents/biosynthesis , Anti-Bacterial Agents/pharmacology , Chromatography, Thin Layer , Microbial Sensitivity Tests , Pigments, Biological , UkraineABSTRACT
Spontaneous and induced variability of Streptomyces aureofaciens strain T[symbol: see text] 633 [symbol: see text] and its variants has been investigated. Correlative relation between the antibiotic formation and colonies morphology was observed. Combined effect of nitrosoguanidine and UV-beams resulted in the obtaining of 27 variants which exceeded activity of strain T[symbol: see text] 633 [symbol: see text] by 30-35% and were stable as to antibiotic biosynthesis during 7-10 generations.
