ABSTRACT
PURPOSE: To analyze the distribution of a tubulins and acetylated alpha tubulins and the chromatin configuration in abnormally fertilized zygotes from a patient with a multifollicular ovarian response after in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). METHODS: Immunofluorescence and phase contrast microscopy was performed in abnormally fertilized zygotes. RESULTS: After phase contrast microscopy analysis, immunofluorescence staining was performed in 20 oocytes that developed > or = 3 pronuclei (PN) and karyomeres after IVF-ICSI. Around 80% of the abnormal zygotes from IVF were the consequence of monospermic fertilizations. Retention of the second polar body (PB) and the presumptive split of > or =1 PN within the cytoplasm were the main events present in most oocytes after IVF-ICSI. CONCLUSIONS: Fluorescence labeling of selected sperm and oocyte components affords a unique view of abnormal fertilized zygotes. Surprisingly, anomalies detected after IVF-ICSI showed similar etiologies in this special group of zygotes.
Subject(s)
Cytoskeleton/ultrastructure , Zygote/pathology , Acetylation , Adult , Chromatin/ultrastructure , Cryopreservation , Cytoskeleton/chemistry , Embryo Transfer , Female , Fertilization in Vitro , Humans , Infant, Newborn , Male , Meiosis , Microscopy, Fluorescence , Microscopy, Phase-Contrast , Ovarian Hyperstimulation Syndrome/etiology , Ovulation Induction/adverse effects , Pregnancy , Protein Processing, Post-Translational , Sperm Injections, Intracytoplasmic , Sperm Tail/chemistry , Sperm Tail/ultrastructure , Spermatozoa , Tubulin/analysis , Tubulin/chemistry , Zygote/chemistrySubject(s)
Humans , Female , Pregnancy , Fertilization in Vitro/methods , Pregnancy, Multiple , Ovarian Follicle , Suction/trendsSubject(s)
Humans , Female , Pregnancy , Pregnancy, Multiple , Fertilization in Vitro/methods , Suction/trends , Ovarian FollicleABSTRACT
Comparar los resultados en técnicas de reproducción asistida (ART) empleando dos vias diferentes de administración de análogos de GnRH (diario vs. depósito9
Subject(s)
Pregnancy , Humans , Female , Argentina , Pituitary Hormone-Releasing Hormones/analysis , Reproductive TechniquesABSTRACT
Comparar los resultados en técnicas de reproducción asistida (ART) empleando dos vias diferentes de administración de análogos de GnRH (diario vs. depósito9(AU)
